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Anti-swine flu transfer factors

A transfer factor, swine influenza virus technology, applied in antiviral agents, antibody medical ingredients, medical preparations containing active ingredients, etc., can solve the problems of incomplete cell fragmentation, large loss of product activity, long time, etc., to prevent Effects of swine influenza virus infection, morbidity reduction, and easy source

Inactive Publication Date: 2010-12-08
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of the above patent application is to directly filter and extract the crushed tissue after freezing and centrifuging. The tissue masher has the defect of incomplete cell crushing, and some processes are cumbersome and take a long time. It is difficult to increase the yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of anti-swine influenza virus transfer factor

[0031] (1) Preparation of virus antigen: Inoculate swine influenza virus into MDBK cells, add 1% trypsin, and culture at 37°C for 24 to 72 hours. The cells were digested, frozen and thawed repeatedly at -20°C for 3 times, ultrasonically broken, centrifuged at 10000rpm for 5min, the precipitate was discarded and the supernatant was collected, formaldehyde inactivated at 4°C, filtered and sterilized, packed and stored at -70°C.

[0032] (2) Purification of virus antigens: After centrifugation of fresh ice venom at 5000 rpm for 30 minutes, the supernatant was centrifuged at 40,000 rpm for 2 hours, and the virus pellet was suspended in TEN; the virus suspension was subjected to sucrose density gradients (20%, 30%, 40%, 50%). , 60%) 20,000 rpm centrifugation for 2 hours to collect each zone; then add an appropriate amount of TEN, centrifuge at 20,000 rpm for 1 hour, the precipitate is dissolved with TEN to obt...

Embodiment 2

[0042] Example 2: Detection of the anti-swine influenza virus transfer factor prepared by the process of the present invention

[0043] The anti-swine influenza virus transfer factor prepared by the process shown in Example 1 was tested as follows:

[0044] (1) Ultraviolet spectrophotometry: The sample obtained above has an absorption peak at 252±2nm, and ABS260 / ABS280>2.0.

[0045] (2) The standard solution is light yellow, with a pH value between 6.0 and 6.5.

[0046] (3) Detection of 20% sulfosalicylic acid: The sample obtained above has no turbidity and precipitation, indicating that the protein reaction is negative and it does not contain macromolecular proteins.

[0047] (4) Bacteriological examination: There is no need for oxygen, anaerobic, saprophytic bacteria and fungi in the samples obtained above.

[0048] (5) Determination of nucleic acid content: The nucleic acid content of the sample obtained above is 589.52μg / mL as determined by the lichenol method.

[0049] (6) Determinat...

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PUM

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Abstract

The invention relates to a process for preparing anti-swine flu virus transfer factors. The transfer factors of the invention can play a role in preventing swine flu and protecting normal body cells from being invaded by swine flu viruses so as to reduce the morbidity. Meanwhile, the transfer factors for treating a swine suffering from swine flu virus infection can effectively improve the clinical symptoms, improve the cure rate and reduce the death rate. The process for preparing the transfer factors comprises the steps of preparing virus antigens, extracting the virus antigens, immunizing the swine by using the virus antigens, extracting the anti-swine flu virus transfer factors from the spleen of the immunized swine and the like.

Description

Technical field [0001] The invention belongs to the field of veterinary medicine, and specifically relates to a preparation process of anti-swine flu transfer factor. Background technique [0002] Swine influenza virus (Swine influenza virus, SIV) can infect pigs of different ages, sexes and breeds throughout the year, causing swine influenza. Swine flu is an acute, febrile, and highly contact respiratory infectious disease. It is clinically characterized by sudden onset, high fever, tearing, coughing, dyspnea failure, and death. Although the mortality rate caused by SIV is very low and the sick pigs can recover quickly, it will hinder the weight gain of the pigs, reduce the meat-to-feed ratio, and decrease the production performance, which will cause great harm to the pig industry. [0003] The occurrence of the disease was first reported in the United States in 1918, and Shope isolated the virus from sick pigs in 1930. So far, the disease has occurred and endemic in all contin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K39/145A61P31/16A61K35/26
Inventor 王小娥
Owner TIANJIN RINGPU BIO TECH
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