Blue crab ptssr17 microsatellite DNA marker testing technique
A technology of swimming crab three warts, DNA labeling, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems such as the swimming crab three warts that have not been seen, and achieve the effect of simple method
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[0016] The following examples describe in detail the present invention's DNA molecular genetic marker technology in Portunus trituberculatus ptssr17 microsatellite core sequence. Firstly, the genomic DNA of Portunus trituberculatus was extracted and diluted for later use; then, using the microsatellite core sequence containing ptssr17 in the Genome Library of Portunus trituberculatus, specific primers were designed at both ends of the sequence; The genomic DNA of individuals in Portunus trituberculatus populations was amplified by PCR, and the PCR products were detected by polyacrylamide gel electrophoresis; the bands that appeared in the products were analyzed to determine the genotype of each individual, and the ptssr17 core of Portunus trituberculatus was obtained. Polymorphism map of high genetic variation in sequence regions.
[0017] 1. Extraction of Genomic DNA of Portunus trituberculatus: Take 100mg of muscle tissue, cut it into pieces, put it into a 1.5ml centrifuge t...
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