Chinese prawn B683 microsatellite label detecting technique

A microsatellite marker, the technology of penaeus chinensis, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as specific genetic markers of microsatellite polymorphism maps of shrimp that have not yet been seen, and achieve the effect of simple method.
CN1488764AInactive Publication Date: 2004-04-14YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2004-04-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a detection technique of Chinese prawn B683 microsatellite marker, which includes the following steps: firstly, extracting Chinese prawn genomic DNA; utilizing microsatellite sequence contained in Chinese prawn genomic library to design specific primer at two ends of its sequence; and making PCR amplification of genomic DNA of Chinese prawn; and making the PCR product undergo the process of denaturing polyacrylamide gel electrophoresis separation, and analyzing band produced by said product and obtaining the genetic polymorphism map of Chinese prawn.
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Description

Technical field

[0001] The invention belongs to the Chinese prawn DNA molecular genetic marker technology, and is a method for detecting the Chinese prawn B683 microsatellite marker technology. Background technique

[0002] Before the present invention was made, there were similar research reports both at home and abroad, "Quick Screening of Recombinant Positive Clones Containing Microsatellites in Chinese Prawns by PCR Method" and "Chinese Shrimp Microsatellites" published by Xu Peng et al. Screening of DNA" and other papers have reported the construction of a partial genome library of Chinese prawns and the screening of microsatellite sequences, and evaluated the core sequences of microsatellites according to the standards proposed by Weber (1990), but his research Results were limited to the acquisition of microsatellite sequences for research and no further application development. In 1999, the French Institute of Marine Development (IFREMER) found 10 microsatellite mar...

Claims

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