Primers and methods for genotype detection of moire grouper using microsatellite marker epinmoyn

A moire grouper, microsatellite marker technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as restricting the development of molecular genetics, and achieve the effect of simple method

Active Publication Date: 2017-12-15
YANTAI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of microsatellite markers of grouper moire, the development of its molecular genetics research is limited, so it is urgent to obtain microsatellite markers for the research and analysis of genetic diversity, individual identification and pedigree authentication of grouper moire. application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers and methods for genotype detection of moire grouper using microsatellite marker epinmoyn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The following examples describe in detail the present invention's DNA molecular genetic marker technology in the moire grouper Epinmoyn microsatellite core sequence. Firstly, the genomic DNA in the blood of the moire grouper was extracted and diluted for later use; then, using the microsatellite core sequence contained in the DNA sequence of the moire grouper, specific primers were designed at both ends of the sequence; PCR amplification is performed on the genomic DNA of individuals in different geographical groups of grouper or moire grouper groups, and denatured polyacrylamide gel electrophoresis detection is performed on the PCR products to determine the genotype of each individual, that is, to obtain moire grouper Genetic polymorphism map of fish.

[0023] 1. Extraction of grouper genomic DNA: Add 100µl of grouper blood into an Eppendorf tube, then add 500µl of cell lysate and 5µl of 20mg / ml proteinase K, shake gently, and leave overnight at 37°C. Then, 600 µl of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a primer and method for detecting the genotype of epinephelus radiatus by use of a microsatellite marker Epinmoyn. The method comprises the following steps: extracting the genome DNA in the blood of epinephelus radiatus and diluting for later use; designing a specific primer by use of the microsatellite core sequence contained in the DNA sequence of epinephelus radiatus; performing PCR amplification on the genome DNA of the individuals in different geographic stocks of epinephelus radiatus or epinephelus radiatus stock by use of the primer, and detecting the PCR product; and analyzing by use of the stripes of the product to determine the genotype of each individual and obtain the genetic polymorphism map of epinephelus radiatus. According to the invention, the genetic variation map of the genetic marker Epinmoyn of epinephelus radiatus can be quickly obtained, the method is simple and convenient, and the obtained result can be used for visually detecting the genotype of each individual of epinephelus radiatus; and the method is applied to the genetic marking, genealogical identification, establishment of the genetic map and the like among the individuals of different geographic stocks or epinephelus radiatus stock.

Description

technical field [0001] The invention belongs to moire grouper ( Epinephelus moara ) The DNA molecular genetic marker technology relates to a technical method for detecting the microsatellite marker Epinmoyn of the moire grouper, specifically a detection method for the microsatellite marker Epinmoyn of the moire grouper. Background technique [0002] Moire Grouper ( Epinephelus moara ) belongs to the order Perciformes, Scorpidae, and the genus Grouper, commonly known as grass spots and oil spots. It is distributed in the western North Pacific Ocean, and it is produced in the East China Sea and South China Sea in China. It has delicious meat, smooth taste, high protein content and rich nutrition, and is favored by consumers. Moire grouper grows fast, has strong adaptability and high economic value, and is suitable for ponds, cages and indoor factory farming. However, the amount of natural resources of moire grouper is small, and it is difficult to meet people's needs. In 2...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6827C12Q1/686C12Q1/6888C12Q2600/156C12Q2525/151
Inventor 刘云国刘凌霄李瑶瑶高永林李彦伸
Owner YANTAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap