Development method and application of siniperca chuatsi sex specific marker

A specific marker and gender technology, applied in the field of fish genetics, can solve the problems of inability to breed sex-reversed populations, great difficulty, etc., and achieve the effect of saving time and economic costs and being easy to operate
CN112359104APending Publication Date: 2021-02-12INST OF AQUATIC LIFE ACAD SINICA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF AQUATIC LIFE ACAD SINICA
Publication Date
2021-02-12

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Abstract

The invention provides a development method and the application of a siniperca chuatsi sex specific marker. The development method comprises the following steps: (1) obtaining male and female siniperca chuatsi specific short sequences as shown in SEQ ID NO.1 by utilizing a high-throughput sequencing method based on a 2b RAD technology; (2) obtaining a male siniperca chuatsi whole genome sequence by using a high-throughput de novo sequencing method; and (3) searching the male siniperca chuatsi specific short sequence obtained in the step (1) in the male siniperca chuatsi whole genome sequence obtained in the step (2) to obtain a longer male siniperca chuatsi specific sequence, namely SEQ ID NO.2, and performing later primer design to be applied to genetic sex identification of siniperca chuatsi. The method has the advantages of quickness, directness, simplicity, convenience and the like, and sex molecular identification can be performed on a large number of samples.
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Description

technical field

[0001] The invention relates to the field of fish genetics, in particular to a development method and application of a mandarin fish sex-specific marker. Background technique

[0002] The study of sex determination in fish has great theoretical significance and application value. It has attracted the attention of scientists for many years and is one of the hotspots in fish research. Fish, as the most primitive vertebrates, is a large group in the history of biological evolution that has rapidly changed to bisexual reproduction, and its sex determination shows primitiveness, complexity and diversity (Kikuchi K, Hamaguchi S. Novel sex-determining genes in fish and sex chromosome evolution. Dev Dyn, 2013, 242:339–353). The primitiveness of fish sex determination is manifested in the fact that fishes do not evolve obvious sex chromosomes and definite sex-determining genes like higher mammals, and most fish have not yet formed obvious sex chromosomes or sex-deter...

Claims

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