Specificity detection primer, detection kit and detection method for schistosoma japonicam sex

Abstract

The invention discloses a specificity detection primer, a detection kit and a detection method for schistosoma japonicam sex, an upstream primer Tsexu2 nucleotide sequence of the specificity primer is 5'-ACGTTAGATACTGCTGTTCA-3, a downstream primer Tsexd2 nucleotide sequence is 5-ATATTGTTCCAAGTACGCAT-3'. In the invention, PCR amplification is carried out on the mould DNA to be detected by the specificity detection primer, the agarose gel electrophoresis is carried out on the amplifying product which is then observed under the ultraviolet light, the amplification strip appears in the positive result, which does not appear in the negative result. In the invention, the specificity detection primer is designed according to female worm specific sequence data of the schistosoma japonicam to establish a rapid, specific, sensitive PCR method used for schistosoma japonicam epidemiology and sex identification. The kit of the invention has simple procedures, strong specificity of the method, high sensitivity and objective result determination, and can be used for sex specific diagnosis of schistosoma japonicam of human and animals.

Description

technical field [0001] The invention relates to the gender identification technology of Schistosoma japonicum. Background technique [0002] Schistosomiasis japonicum is a serious zoonotic parasitism caused by Schistosoma japonicum parasitizing in the small blood vessels of the portal vein system of humans and cattle, sheep, pigs, horses, dogs, cats, rabbits, rodents and various mammals Sexual flukes. The disease is characterized by acute or chronic enteritis, liver cirrhosis, severe diarrhea, anemia, and emaciation, which seriously affects human health and the development of animal husbandry economy. It is mainly distributed in some countries in the Western Pacific region such as China, Japan, the Philippines and Indonesia. Among them, Japan has formally applied to WHO in 1997, declaring it a non-endemic area for schistosomiasis. In my country, the endemic areas are located in the Yangtze River Basin and the areas to the south. Except for Shanghai, Zhejiang, Fujian, Guan...

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Problems solved by technology

[0003] At present, the diagnosis of schistosomiasis mainly relies on fecal pathogen detection and immunological methods, but the detection rate of feces detection is low, and it is easy to miss the diagnosis, and the immunological methods established by each laboratory are carried out according to the local actual conditions. Its widespread promotion and comparability still need further research; the prevention and treatment of schistosomiasis mainly relies on drug control and vaccine prevention, but the drug resistance of drug development and the long-term and complexity of vaccine development have become the bottleneck of the disease control

Current studies have found that inhibiting or preventing female and male symbiosis of Schistosoma japonicum or inhibiting the development of female worms is a new way to effectively prevent and treat the disease, but it is still unclear at which stage the female and male worms of Schistosoma japonicum differentiate

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