Specificity detection primer, detection kit and detection method for schistosoma japonicam sex

A technique for detecting primers and detection methods, which is applied in the field of gender identification of Schistosoma japonicum, can solve the problems of low detection rate of feces detection, easy missed diagnosis, unclear differentiation of female and male worms, etc., and achieves objective and strong specificity in result determination , the effect of programmatic operation
CN101597645AInactive Publication Date: 2009-12-09SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2009-12-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a specificity detection primer, a detection kit and a detection method for schistosoma japonicam sex, an upstream primer Tsexu2 nucleotide sequence of the specificity primer is 5'-ACGTTAGATACTGCTGTTCA-3, a downstream primer Tsexd2 nucleotide sequence is 5-ATATTGTTCCAAGTACGCAT-3'. In the invention, PCR amplification is carried out on the mould DNA to be detected by the specificity detection primer, the agarose gel electrophoresis is carried out on the amplifying product which is then observed under the ultraviolet light, the amplification strip appears in the positive result, which does not appear in the negative result. In the invention, the specificity detection primer is designed according to female worm specific sequence data of the schistosoma japonicam to establish a rapid, specific, sensitive PCR method used for schistosoma japonicam epidemiology and sex identification. The kit of the invention has simple procedures, strong specificity of the method, high sensitivity and objective result determination, and can be used for sex specific diagnosis of schistosoma japonicam of human and animals.
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Description

technical field

[0001] The invention relates to the gender identification technology of Schistosoma japonicum. Background technique

[0002] Schistosomiasis japonicum is a serious zoonotic parasitism caused by Schistosoma japonicum parasitizing in the small blood vessels of the portal vein system of humans and cattle, sheep, pigs, horses, dogs, cats, rabbits, rodents and various mammals Sexual flukes. The disease is characterized by acute or chronic enteritis, liver cirrhosis, severe diarrhea, anemia, and emaciation, which seriously affects human health and the development of animal husbandry economy. It is mainly distributed in some countries in the Western Pacific region such as China, Japan, the Philippines and Indonesia. Among them, Japan has formally applied to WHO in 1997, declaring it a non-endemic area for schistosomiasis. In my country, the endemic areas are located in the Yangtze River Basin and the areas to the south. Except for Shanghai, Zhejiang, Fujian, Guan...

Claims

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