Specific dna fragments for sex identification of silktail fish and their application

A silk-tailed, specific technology, which is applied in the field of fish sex identification, can solve the problems of limited genetic information resources, inability to detect sex-specific DNA molecular markers, etc., and achieve the effect of less damage to the fish body.
CN109880893BActive Publication Date: 2020-02-04INST OF AQUATIC LIFE ACAD SINICA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF AQUATIC LIFE ACAD SINICA
Publication Date
2020-02-04

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Abstract

The invention belongs to the field of fish sex identification in the field of aquaculture, and particularly relates to a specific DNA fragment for hemibagrus wyckioides sex determination and application. On the basis of combining a low coverage whole genome survey technology with a 2b-RAD-seq technology, and by means of a genome walking technology, hemibagrus wyckioides male sex specific markerisdeveloped, the specific DNA fragment is shown in SEQIDNO.2 or SEQIDNO.1. For the specific sequence, the sex of hemibagrus wyckioides can be identified by means of conventional PCR amplification, and it is verified thatthe accuracy rate reaches 100%. Compared with previous anatomical detection or genital swelling observation, thetechnology is accurate, simple, rapid, less in harmful to fish body and the like, and help is provided for hemibagrus wyckioides sex control breeding, wild resource protection and the development of the aquaculture industry.
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Description

technical field

[0001] The invention belongs to the field of fish sex identification in the field of aquaculture, and in particular relates to a specific DNA fragment for sex identification of silktail fish and its application. Background technique

[0002] Hemibagrus wyckioides is a sub-wide-haline bottom-dwelling fish that is only distributed in the Lancang River system and the Mekong River Basin in the lower reaches of my country. Silk-tailed porcupine has strong adaptability, easy reproduction, strong disease resistance, delicious meat, and high nutritional value. It is a famous and high-quality new breed that is worth developing and promoting. It is one of the most valuable wild fish in Yunnan. However, due to overfishing and the construction of hydropower stations on the main and tributary streams of the Lancang River, wild resources are becoming less and less, so wild resources need to be protected. At the same time, like many commercial fish species, there is a sign...

Claims

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