Patinopecten yessoensis living body sex identification method based on DNA molecular markers

A DNA molecule and technology of Ezo scallop, applied in the field of live sex identification of Ezo scallop, can solve the problem that the method of sex identification of live Ezo scallop has not yet been established, and achieve the effect of high accuracy
CN110607376AActive Publication Date: 2019-12-24OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2019-12-24

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Abstract

The invention provides a patinopecten yessoensis living body sex identification method based on DNA molecular markers. The patinopecten yessoensis living body sex identification method based on the DNA molecular markers comprises the steps that a small amount of gill filaments is obtained from the patinopecten yessoensis through a living body, DNA is extracted, PCR amplifies the sex-specific DNA molecular markers, whether a target band exists in a PCR product or not is detected by electrophoresis, and patinopecten yessoensis living body sex identification is achieved. The sex-specific DNA molecular markers are unique to female, and a nucleotide fragment sequence of the sex-specific DNA molecular markers is SEQ ID NO:1. PCR primers are used for amplifying the molecular markers, and the sexof patinopecten yessoensis is identified based on the agarose gel electrophoresis result; and a sample amplifying a 1859bp specific band is female, and samples without the band are male. The patinopecten yessoensis living body sex identification can be realized, the method is simple, the result is accurate, and the method is helpful to speed up the breeding process of the patinopecten yessoensis.
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Description

technical field

[0001] The invention belongs to the technical field of sex identification in aquaculture, and in particular relates to a method for sex identification of live scallops based on DNA molecular markers. Background technique

[0002] Scallops are one of the most important maricultured shellfish in northern my country. At present, large-scale farming has been formed in the Bohai Sea and the northern Yellow Sea, creating an output value of billions of yuan in the past 10 years. Ezo scallops are dioecious, and generally reach sexual maturity in 1-2 years. When the gonads enter the growth and maturity stages, the sex of the scallops can be identified with the naked eye. However, when the gonads are in the undifferentiated, telogen, or proliferative stages, it is difficult to identify the sex with the naked eye, and it is necessary to dissect the gonads, and perform gender identification through histological sections or quantitative analysis of gene expression. Howev...

Claims

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