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Special molecular marker for sex of verasper variegates, and genetic sex identification method

A technology of flounder and molecular markers, which can be applied to biochemical equipment and methods, microbial measurement/inspection, etc., and can solve problems that have not been reported

Inactive Publication Date: 2011-04-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
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Problems solved by technology

However, there are no reports at home and abroad about sex-specific molecular markers and molecular biology techniques for genetic sex identification of star flounder.

Method used

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  • Special molecular marker for sex of verasper variegates, and genetic sex identification method
  • Special molecular marker for sex of verasper variegates, and genetic sex identification method
  • Special molecular marker for sex of verasper variegates, and genetic sex identification method

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Embodiment Construction

[0039] Using modern molecular biology techniques, sex-specific molecular markers were screened out, and sex-specific AFLP fragments were cloned using molecular cloning techniques to establish a PCR analysis technique for genetic sex identification of the star flounder. Seedlings, all-female seedlings are cultured to increase aquaculture production and improve the economic benefits of breeding, and truly develop the star flounder into an excellent breeding species accepted by the majority of farmers, and promote the development of the star flounder aquaculture industry and seawater The upgrading of fish breeding species has important practical significance and huge economic benefits.

[0040] Below, the technical content of the present invention will be described in detail in conjunction with the accompanying drawings through the screening of female-specific AFLP molecular markers and the establishment of a genetic sex identification method:

[0041] The technical contents of t...

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Abstract

The invention relates to spotted halibut sex-specific molecular markers and a genetic sex detection method. The method comprises the following steps of extraction of fin ray DNA, AFLP analysis of DNA of genomes, screening and sequencing of the sex-specific AFLP markers, and establishment of the genetic sex identification method. The genetic sex detection method establishes the spotted halibut finray DNA extraction method and the AFLP analysis technology, screens out two female-specific AFLP molecular markers the molecular weight of which is respectively 533 bp and 218 bp, obtains nucleotide sequences of the two AFLP markers, and establishes the molecule marking technology of spotted halibut sex-specific identification. The method has the advanced, accurate and reliable characteristics, has important application value in control of spotted halibut sex and production of full-female seedlings, and simultaneously has wide application prospect in genetic sex identification and sex controland research of other fish varieties.

Description

Technical field: [0001] The invention belongs to fish genetic sex identification and sex control technology in the technical field of aquatic organisms, and is a molecular biological method for fish genetic sex identification that can be applied in fish parthenogenesis. Background technique: [0002] my country is rich in fish resources, and many of them have gender differences in growth rate. For example, male tilapias grow about 40% faster than females, while seawater fish such as flounder have females that grow faster than males. Individual growth is 40%-100% faster. Therefore, research on genetic sex detection and sex control of these fishes has important scientific significance, and has great application potential and promotion prospects. [0003] Verasper variegates (Verasper variegates) is a rare and valuable economic seawater fish unique to my country. It belongs to offshore cold-water fish. It is distributed in the northern coastal areas of my country, mostly in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈松林马洪雨田永胜
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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