Special molecular marker for sex of verasper variegates, and genetic sex identification method

A technology of flounder and molecular markers, which can be applied to biochemical equipment and methods, microbial measurement/inspection, etc., and can solve problems that have not been reported
CN101429557BInactive Publication Date: 2011-04-06YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2011-04-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to spotted halibut sex-specific molecular markers and a genetic sex detection method. The method comprises the following steps of extraction of fin ray DNA, AFLP analysis of DNA of genomes, screening and sequencing of the sex-specific AFLP markers, and establishment of the genetic sex identification method. The genetic sex detection method establishes the spotted halibut finray DNA extraction method and the AFLP analysis technology, screens out two female-specific AFLP molecular markers the molecular weight of which is respectively 533 bp and 218 bp, obtains nucleotide sequences of the two AFLP markers, and establishes the molecule marking technology of spotted halibut sex-specific identification. The method has the advanced, accurate and reliable characteristics, has important application value in control of spotted halibut sex and production of full-female seedlings, and simultaneously has wide application prospect in genetic sex identification and sex controland research of other fish varieties.
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Description

Technical field:

[0001] The invention belongs to fish genetic sex identification and sex control technology in the technical field of aquatic organisms, and is a molecular biological method for fish genetic sex identification that can be applied in fish parthenogenesis. Background technique:

[0002] my country is rich in fish resources, and many of them have gender differences in growth rate. For example, male tilapias grow about 40% faster than females, while seawater fish such as flounder have females that grow faster than males. Individual growth is 40%-100% faster. Therefore, research on genetic sex detection and sex control of these fishes has important scientific significance, and has great application potential and promotion prospects.

[0003] Verasper variegates (Verasper variegates) is a rare and valuable economic seawater fish unique to my country. It belongs to offshore cold-water fish. It is distributed in the northern coastal areas of my country, mostly in the ...

Claims

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