Sialic acid assay kit and assay method thereof

A technology of sialic acid and kit, which is applied in the field of sialic acid determination kit and its determination, can solve the problem of unsatisfactory anti-interference ability and achieve the effect of enhancing anti-interference ability, enhancing stability and good accuracy

Inactive Publication Date: 2018-11-23
广州市伊川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are many types of stabilizers used in existing kits. Although...

Method used

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  • Sialic acid assay kit and assay method thereof
  • Sialic acid assay kit and assay method thereof
  • Sialic acid assay kit and assay method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Sialic acid assay kit (formula 2)

[0022] The sialic acid assay kit includes reagent R1 and reagent R2, and the reagent R1 includes the following components and their concentrations: Tris 12g / L, HCL 8ml / L, neuraminidase 1KU / L, lactate Hydrogenase 2KU / L, NADH 0.5mmol / L, potassium sorbate 1g / L; the reagent R2 includes the following components and their concentrations: Tris 12g / L, HCL 8ml / L, N-acetyl neuron Amino acid aldolase 2KU / L, sucrose 50g / L, triethanolamine 1ml / L, potassium sorbate 1g / L.

Embodiment 2

[0023] Example two sialic acid assay kit

[0024] The sialic acid assay kit includes reagent R1 and reagent R2, and the reagent R1 includes the following components and their concentrations: Tris 10g / L, HCL 6ml / L, neuraminidase 1KU / L, lactate Hydrogenase 1KU / L, NADH 0.5mmol / L, potassium sorbate 0.8g / L; the reagent R2 includes the following components and their concentrations: Tris 10g / L, HCL 6ml / L, N-acetyl neuro Amino acid aldolase 3KU / L, sucrose 70g / L, triethanolamine 1.2ml / L, potassium sorbate 0.8g / L.

Embodiment 3

[0025] Example three sialic acid assay kit

[0026] The sialic acid assay kit includes reagent R1 and reagent R2, and the reagent R1 includes the following components and their concentrations: Tris 15g / L, HCL 10ml / L, neuraminidase 2KU / L, lactate Hydrogenase 2KU / L, NADH 1mmol / L, potassium sorbate 1.2g / L; the reagent R2 includes the following components and their concentrations: Tris 15g / L, HCL 10ml / L, N-acetylneuramin Acid aldolase 2KU / L, sucrose 40g / L, triethanolamine 1ml / L, potassium sorbate 1.2g / L.

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Abstract

The invention provides a sialic acid assay kit, comprising a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components with corresponding concentrations: 8-20g/L tris(hydroxymethyl) aminomethane, 5-15ml/L HCL, 0.5-5KU/L neuraminidase, 0.5-5KU/L lactate dehydrogenase and 0.1-2mmol/L NADH; the reagent R2 is prepared from the following components with correspondingconcentrations: 8-20g/L tris(hydroxymethyl) aminomethane, 5-15ml/L HCL, 0.5-5KU/L N-acetylneuraminic acid aldolase, 25-100g/L sucrose and 0.6-1.2 ml/L triethanolamine. The invention belongs to the technical field of biological detection. The sialic acid assay kit provided by the invention significantly enhances the anti-interference ability while improving the stability, can significantly reducethe interference of bilirubin, hemoglobin, triglyceride and the like, and is good in accuracy when being used for the determination of sialic acid.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a sialic acid determination kit and a determination method thereof. Background technique [0002] Sialic acid (Sialic acid, SA), also known as N-acetylneuraminic acid, is one of a variety of natural neuraminic acid derivatives. Its parent structure is an endocyclic amino acid composed of nine carbon atoms. It is a general term for a family of compounds. Sialic acid is an important part of cell membrane glycoprotein, widely distributed in animals, sialic acid in human serum includes free sialic acid and bound sialic acid, the content of free sialic acid is low, bound sialic acid bound to lipoprotein and glycoprotein Acid is the main form of sialic acid in serum. [0003] Sialic acid is related to many biological functions of organisms, and is closely related to cell malignant transformation, cancer metastasis, invasion, loss of contact inhibition, decreas...

Claims

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Application Information

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IPC IPC(8): C12Q1/527
CPCC12Q1/527G01N2333/988
Inventor 刘光华杨玉军陈楚华刘秋明
Owner 广州市伊川生物科技有限公司
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