P-carboxypropanamide phenyl silica, its co-immobilized l-lactate dehydrogenase complex and its preparation method

A carboxylate propionamide, lactate dehydrogenase technology, applied in the preparation of organic compounds, the preparation of carboxylate, chemical instruments and methods, etc., can solve the problems of obtaining electrons, catalyst deactivation, low catalytic efficiency, etc. The effect of extending the scope of application

Inactive Publication Date: 2017-06-23
SHANGHAI UNIV
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Problems solved by technology

[0004] Simultaneous immobilization of enzymes and cofactors is an effective method to realize the reuse of catalysts. However, studies have shown that the catalytic efficiency of this co-immobilization method is not high, and often leads to catalyst deactivation, which may be due to the unfavorable The interaction of NADH with the active site of the enzyme, so that the enzyme cannot obtain electrons from NADH, and catalyzes the conversion of sodium pyruvate into lactate

Method used

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  • P-carboxypropanamide phenyl silica, its co-immobilized l-lactate dehydrogenase complex and its preparation method
  • P-carboxypropanamide phenyl silica, its co-immobilized l-lactate dehydrogenase complex and its preparation method
  • P-carboxypropanamide phenyl silica, its co-immobilized l-lactate dehydrogenase complex and its preparation method

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Embodiment 1

[0050] Example 1: CPAPhS-LDH and NADH-CPAPhS-LDH layered compounds, see figure 1 and figure 2 , the preparation;

[0051] (1) Prepare p-aminophenyl silica-dodecylsulfate layered compound in advance; its preparation method is: first dissolve 2.88mmol of sodium laurylsulfate into 250ml of deionized water, and then add 2.74mmol of p-aminophenyltrimethoxysilane, then slowly dropwise add 0.5mol / L hydrochloric acid to adjust the pH value of the mixed solution to 2, and magnetically stir at room temperature for 12 days to carry out the sol-gel process; Washing with deionized water and ethanol, and drying in vacuum to obtain p-aminophenyl silicon oxide-dodecylsulfate layered structure compound;

[0052] In order to prove the chemical and three-dimensional structure of p-aminophenyl silica-dodecylsulfuric acid nanocomposite layered structure, a series of analytical experiments were carried out. Firstly, octaalkyl sulfate anion is used, and chloride anion carries out anion exchange ...

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Abstract

The invention relates to p-carboxyl propionamidophenyl silicon oxide, a co-immobilized L-lactic dehydrogenase compound and a preparation method thereof. The compound is formed by using p-carboxyl propionamidophenyl silicon oxide with regularly-arranged carboxyl groups as a skeleton, and grafting L-lactic dehydrogenase and cofactor NADH. The novel organic / inorganic composite two-dimension lamellar compound overcomes the disadvantages that conventional montmorillonite and hydrotalcite lamellar materials are difficult to control a layer space environment; an application scope of functional enzyme is greatly broadened by developing and synthesizing the organic / inorganic composite lamellar compound with the functional enzyme; and a series of novel biological functional composite materials with various performances are developed.

Description

technical field [0001] The invention relates to a p-carboxylate propionamide phenyl silicon oxide with regularly arranged carboxyl groups, its co-immobilized L-lactate dehydrogenase complex and a preparation method thereof. Background technique [0002] Lactate dehydrogenase (English: Lactate dehydrogenase; EC 1.1.1.27) is an NAD-dependent enzyme widely found in various organisms, including plants and animals. L-lactate dehydrogenase (LDH) catalyzes the reduction and oxidation reaction between pyruvate and lactic acid, promotes the reaction of lactic acid to pyruvate under alkaline conditions, and promotes the conversion of pyruvate to lactic acid under neutral conditions (for reverse reaction). LDH is an important enzyme involved in the anaerobic glycolysis and gluconeogenesis of sugar. Since LDH exists in almost all somatic cells and is generally highly active in human tissues, the level of LDH content in various tissues has very important clinical significance for the d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F7/10B01J31/02C07C59/08C07C51/367C12N11/02
Inventor 姚建李龙夏恒恒孙开莲刘婉君
Owner SHANGHAI UNIV
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