Method for preparing feather meal additive via mixed solid-state fermentation

Abstract

The invention relates to a method for preparing feather meal additive via mixed solid-state fermentation, wherein adopted strains are bacillus licheniformis, bacillus alcalophilus and bacillus subtilis with preservation numbers of CGMCC (China General Microbiological Culture Collection Center) No.1699, CGMCC No.5313 and CGMCC No. 1398 respectively. According to the invention, as solid fermentation is adopted, broken feathers are processed to prepare solid fermentation medium; and the three strains are mixed according to a certain proportion and then inoculated into the fermentation medium according to suitable inoculum size. According to the method provided by the invention, the hydrolysis efficiency of feather can be effectively improved and the content of soluble protein can be up to 9.42mg / g, so that a high-nutrition feed additive is provided for the feed industry and the resource waste and environment pollution are effectively avoided to a certain extent.

Description

Technical field: [0001] The invention belongs to the field of microorganism application, and relates to a biodegradation method of feather meal keratin, in particular to a method for preparing feather meal additive by solid-state fermentation of mixed bacteria. Background technique: [0002] In recent years, with the continuous development of my country's poultry industry, a large amount of poultry waste has been produced, among which feather waste is the most. The main component of feather is keratin, its crude protein content accounts for more than 80% of feather content, amino acid content also accounts for about 70%, and contains a large number of macro elements, trace elements, vitamins and some unknown growth factors. A good source of feed protein that can replace or partially replace fishmeal. At present, the treatment methods of feather keratin mainly include traditional hydrolysis method and biotechnology method. Although traditional high-temperature cooking or ch...

AI Technical Summary

Problems solved by technology

[0003] At present, domestic and foreign research on microbial hydrolysis of feathers mainly focuses on the decomposition of feathers by liquid submerged fermentation, but it is mainly aimed at the treatment of environmental waste, and the recycling rate of decomposition products is low, wasting a lot of amino acids and soluble proteins.

It also requires a large number of steps to be used as poultry feed, consumes energy and time, and produces a large amount of waste liquid, which will cause certain pollution to the environment

In addition, domestic and foreign research on feather hydrolysis focuses more on the decomposition of feathers by a single strain. A single strain mainly secretes keratinase to decompose feathers mainly composed of keratin. The enzyme production is small and the enzyme activity is low. Low, can not efficiently and quickly hydrolyze keratin