A method for pretreating lignocellulosic raw materials with concentrated phosphoric acid and hydrogen peroxide for enzymatic hydrolysis

A technology of lignocellulose and hydrogen peroxide, applied in the direction of biofuels, fermentation, etc., can solve the problems of poor pretreatment effect, influence on fermentation, low energy consumption and cost of pretreatment process, and achieve reduction of energy consumption and cost, The effect of enzymatic hydrolysis efficiency improvement and hydrolysis time shortening

Active Publication Date: 2016-04-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as an efficient lignocellulose pretreatment technology, the following aspects must be considered comprehensively while ensuring the feasibility of the technology: 1) high adaptability to different raw materials; 2) pretreated wood Cellulose has good hydrolysis performance; 3) There is no significant loss of sugar in the pretreatment process; 4) The toxic by-products in the pretreatment process are low; 5) After pretreatment, it is suitable for high substrate concentration hydrolysis; 6) It is suitable for subsequent fermentation; 7) The energy consumption and cost of the pretreatment process are relatively low; however, the existing lignocellulose pretreatment methods cannot simultaneously meet the high-efficiency pretreatment standards to varying degrees
Alkali treatment (including ammonia explosion, AFEX) can effectively reduce the lignin content of crop straw, but the pretreatment effect on cork materials with high structural density and lignin content is poor; steam (gas explosion) pretreatment The treatment method is more suitable for the dissolution and removal of hemicellulose with xylan as the main chain, but the pretreatment effect is not good for hemicellulose with mannan as the main chain
The pretreatment methods with the participation of high temperature, including steam pretreatment, hydrothermal method, organic solvent method, etc., will produce toxic inhibitors in the pretreatment process, which seriously affects the subsequent fermentation; in addition, most of the existing pretreatment methods , the damage to the lignocellulose spatial structure is limited, and the raw material after pretreatment still has a high degree of polymerization or crystallinity, resulting in a low concentration of the substrate for subsequent enzymatic hydrolysis, and it is impossible to obtain a hydrolyzate with a high sugar concentration
Recently, the use of ionic solution pretreatment can effectively improve the degree of polymerization and crystallinity of cellulose in raw materials, and improve the rheological properties of the mechanism in the enzymatic hydrolysis process, but the potential inhibition and toxicity of ionic solution to subsequent enzymatic hydrolysis and fermentation still need to be investigated. Further research, and the reduction of the preparation cost of the ionic solution still needs further consideration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Example 1 Different temperature conditions are used for lignocellulosic raw material pretreatment

[0022] (1) Materials

[0023] 1. Wheat straw, crushed by a pulverizer and passed through a 40-mesh sieve.

[0024] 2. Cellulase (Celluclast1.5L) and β-glucosidase (Novozyme188) were purchased from Novozymes.

[0025] (2) Method

[0026] 1. Put 50g of wheat straw powder into the reactor, and add 500g of phosphoric acid and hydrogen peroxide mixture into the reactor according to the solid-to-liquid ratio of 1:10, wherein the mass fraction of phosphoric acid is 80%, and the mass fraction of hydrogen peroxide is 1.77 %, set different pretreatment reaction temperatures as 20°C, 30°C, 40°C, 50°C, and the reaction time as 5h for pretreatment.

[0027] 2. Add distilled water (about 5500 mL) 10 times the mass of the reaction solution system to the mixture obtained in step 1 to terminate the reaction.

[0028] 3. The mixture obtained in step 2 is subjected to vacuum filtration ...

example 2

[0032] Example 2 Different pretreatment time is used for lignocellulosic raw material pretreatment

[0033] (1) Materials

[0034] 1. Wheat straw is crushed by a pulverizer and passed through a 40-mesh sieve.

[0035] 2. Cellulase (Celluclast1.5L) and β-glucosidase (Novozyme188) were purchased from Novozymes.

[0036] (2) Method

[0037]1. Put 50g of wheat straw powder into the reactor, and add 500g of phosphoric acid and hydrogen peroxide mixture into the reactor according to the solid-to-liquid ratio of 1:10, wherein the mass fraction of phosphoric acid is 80%, and the mass fraction of hydrogen peroxide is 1.77 %, set different pretreatment reaction times as 1h, 3h, 5h, and the reaction temperature is 50°C for pretreatment.

[0038] 2. Add distilled water (about 5500 mL) 10 times the mass of the reaction solution system to the mixture obtained in step 1 to terminate the reaction.

[0039] 3. The mixture obtained in step 2 is subjected to vacuum filtration to obtain the p...

example 3

[0043] Example 3 Different phosphoric acid-hydrogen peroxide ratios are used for lignocellulosic raw material pretreatment

[0044] (1) Materials

[0045] 1. Wheat straw, crushed by a pulverizer and passed through a 40-mesh sieve.

[0046] 2. Cellulase (Celluclast1.5L) and β-glucosidase (Novozyme188) were purchased from Novozymes.

[0047] (2) Method

[0048] 1. Put 50g of wheat straw powder into the reactor, and add 500g of phosphoric acid and hydrogen peroxide mixture into the reactor according to the solid-to-liquid ratio of 1:10, and the mass fraction of phosphoric acid is controlled at 80%, 70%, 60% %, corresponding to the mass fraction control of hydrogen peroxide is 1.77%, 5.29%, 8.82%. Set the different pretreatment reaction time to 5h, and the reaction temperature to 50°C for pretreatment.

[0049] 2. Add distilled water (about 5500 mL) 10 times the mass of the reaction solution system to the mixture obtained in step 1 to terminate the reaction.

[0050] 3. The m...

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Abstract

The invention discloses a method for pre-treating a lignocelluloses raw material for enzyme hydrolysis in combination of strong phosphoric acid and hydrogen peroxide. The method comprises the following steps: using strong phosphoric acid as a solvent and hydrogen peroxide as a catalyst to remove lignin and hemicellulose in the lignocelluloses raw material, adding water in the solution to regenerate cellulose in the solution so as to obtain regenerated cellulose which is low in crystalline and polymerization degree and suitable for enzyme hydrolysis, using the regenerated cellulose after filtering and separating for the enzyme hydrolysis to produce fermentable carbohydrate, wherein the hydrolysis efficiency is 75-100%. The method is suitable for lignocelluloses raw materials in different types such as crop stalks, woods and bamboos, wild herbs or woody and the like, the method is simple in operation, low in energy consumption, clean and non-pollution, low in requirement on grain size of raw materials, and strong in raw material adaptability, and the pre-treatment effect is perfect.

Description

technical field [0001] The present invention relates to a pretreatment method for lignocellulose raw materials used in the production of cellulose ethanol and other bio-based products that use cellulose glycosyl as raw materials, in particular to a method for pretreatment of lignocellulose with concentrated phosphoric acid combined with hydrogen peroxide A method for enzymatic hydrolysis of similar raw materials. Background technique [0002] At present, the main energy supporting the development of the world economy is still oil, natural gas and raw coal. The use of fossil energy accelerates the deterioration of the ecological environment and poses a huge threat to human survival. According to the primary energy consumption structure of various countries in the world in 2012, it can be seen that the proportion of clean energy in the energy consumption of various countries is very small, less than 1.5% in China, which shows that my country is facing a particularly prominent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14
CPCY02E50/10
Inventor 沈飞王卿曾咏梅杨刚张延宗蔺丽丽邓仕槐王莉淋肖鸿李远伟
Owner SICHUAN AGRI UNIV
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