Constitutive mannase producing Bacillus alcalophilus NTT33C6 and application thereof

A technology of NTT33C6 and alkalophilic bacillus, applied in the field of microorganisms, can solve the problems of high production cost, high processing cost, high consumption of raw materials and energy consumption, etc., achieve high production stability, avoid secondary pollution, and reduce production costs Effect

Active Publication Date: 2017-02-15
曹军卫 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems of the chemical degumming method: first, the process is long, consumes a lot of raw materials and energy consumption, resulting in high production costs; second, the use of high-concentration acid, alkali, oxidant, etc., causes great damage to the fiber and poor fiber quality , so it is difficult to meet the requirements of spinning high-count yarn; the third is that the COD of degumming sewage produced Cr (chemical oxygen demand) can be as high as 15000-25000mg/mL, and BOD 5 /COD Cr <0.3, belongs to very difficult sewage, in order to meet the discharge standard, it needs to be treated after a large amount of dilution, so the treatment cost is high, and it is difficult to meet the standard, so it will seriously pollute the environment (Wu Junnan, Hao Xinmin, Tang Zongliu, etc. Hemp fiber high temperature— F

Method used

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  • Constitutive mannase producing Bacillus alcalophilus NTT33C6 and application thereof
  • Constitutive mannase producing Bacillus alcalophilus NTT33C6 and application thereof
  • Constitutive mannase producing Bacillus alcalophilus NTT33C6 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The isolation and screening method of alkalophilic bacillus NTT33C6, its steps are:

[0054] 1) Sample collection

[0055] Soil sampling was carried out from the Zhelimu League area of ​​Inner Mongolia Autonomous Region, China. 1g of the soil sample was suspended in sterile water and mixed evenly; the supernatant was put into the separation medium, and its composition was: glucose 10g, peptone 5g, yeast extract 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.2g, agar 18g, water 1000mL, with Na 2 CO 3 Adjust the pH to 10-11; incubate at 28°C for 24-48 hours.

[0056] 2) Separation and purification

[0057] Select a single colony in the isolation medium and transfer it to the locust bean gum solid medium, the composition of which is locust bean gum 10g, yeast extract 5g, peptone 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2O 0.1g, agar 18g, water 1000mL, with Na 2 CO 3 Adjust the pH to 10-11; select the bacterial strains that produce a transparent circle around the colony, and place th...

Embodiment 2

[0076] The application and effect analysis of Bacillus alkalophilus NTT33C6 in the biological degumming of cannabis, the steps are:

[0077] 1) The obtained mutant strain NTT33C6 was cultured in locust bean gum liquid medium at 37°C, 200 rpm, and activated for 36-48 hours;

[0078] 2) transfer to the culture medium containing hemp respectively, shake at 37°C, 200rpm, and cultivate for 6-8 hours; 4 ) 2 SO 4 1.5g, K 2 HPO 4 0.2g, MgSO 4 ·7H 2 O0.25g, water 150mL, and use Na 2 CO 3 Adjust pH to 10-11;

[0079] 3) Observe the fiber dispersion in the culture medium, measure the residual glue rate, and analyze the lipid waxy, water-soluble, pectin, hemicellulose, lignin and cellulose content of the hemp raw hemp and the fiber after biological degumming [Chinese people Republic "Quantitative Analysis Method for Chemical Components of Ramie" (GB5889-86)]. Its measurement method and calculation formula are as follows:

[0080] ①Determination of moisture content of raw hemp ...

Embodiment 3

[0140] The application of alkalophilic bacillus NTT33C6 in cannabis production, its steps are:

[0141] 1) carry out biological degumming to cannabis according to the method described in step 1) and 2) in Example 2;

[0142] 2) The hemp after biological degumming is dispersed like cotton, after washing the hemp once, in a solution containing 0.2-0.4% mass concentration of NaOH and 0.1% mass concentration of sodium tripolyphosphate, 0.2Mpa, scouring 1-2 Hour.

[0143] 3) According to the general treatment method of chemical degumming (Jingang. Research progress of hemp degumming method [J]. Journal of Nanjing Forestry University, 2009, 33 (4): 140-144.) Washing, beating hemp, bleaching (available chlorine 0.5-0.8g / L), water washing, pickling (pH2.5), water washing, drying, oiling, drying, and drying to obtain fine-dried hemp fiber that meets national quality standards and production needs.

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Abstract

The invention discloses Bacillus alcalophilus NTT33C6, collected under CCTCC M2016316 and capable of producing constitutive alkaline Beta-mannase; the bacterium is rod shaped, is 0.5-0.65*2.6-3.4 Mum in size, with lateral flagella, is mobile and produces elliptical endogenous spores; the bacterium is aerobic, can form a circular colony, has smooth surface, and is concentric; Bacillus alcalophilus NTT33C6 is capable of biologically degumming hemp, with a degumming rate in 6-8 hours reaching 70% and above, and capable of biologically degumming hemp with skin not removed, with a degumming rate in 6-8 hours reaching 80% and above; degumming wastewater is easy to treat; biological degumming and chemical degumming are jointly used, and refined dried hemp with certain need on residual gum content can be produced on production demand.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an alkalophilic bacillus NTT33C6 producing constitutive mannanase, and at the same time relates to a method for separating and screening the alkalophilic bacillus NTT33C6, and also relates to the use of the strain in cannabis biological degumming and cannabis production use. Background technique [0002] Marijuana (Cannabis sativa L.) belongs to the family Moraceae (Maraceae) and belongs to the genus Cannabis. Annual erect herb, 1-4 meters high. Hemp has the biological characteristics of barren tolerance, strong stress resistance, wide adaptability, light-loving (especially short-day) and high photosynthetic efficiency. [0003] China has a long history of growing marijuana and using hemp products. Fragments of marijuana from the Shang Dynasty have been unearthed in Henan, my country, and hemp seeds unearthed in Linxi, Dongxiang, Gansu are also more than 5,00...

Claims

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Application Information

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IPC IPC(8): C12N1/20D01C1/04C12R1/07
CPCC12N9/2491C12Y302/01025C12N1/205C12R2001/07D01C1/04
Inventor 曹军卫朱雨兰夏晨阳涂毅
Owner 曹军卫
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