Method for producing alkaline protease

Abstract

The invention relates to a method for producing an alkali protease. The classification name of bacterial strains is Bacillus alcalophilus, and the preservation number is CGMCC No.5313. According to the fermentation process in the invention, clear liquid fermentation is combined with fed-batch fermentation, a cottonseed cake powder enzymatic hydrolysate is prepared from crude cottonseed cake powder, and a fed-batch carbon nitrogen source fermentation mode is adopted, so the fermentation enzyme activity is effectively improved, the enzyme activity of a 7L fermentation tank, which reaches 41000U / mL, is 13.9% higher than the original enzyme activity of 36000U / mL of the 7L fermentation tank, the late enzyme separation purifying difficulty is reduced, the production cost is reduced, and good economic benefits are obtained.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a production method of alkaline protease. Background technique [0002] Alkaline Protease belongs to the serine proteolytic enzymes in endopeptidases, which can hydrolyze protein peptide bonds under alkaline conditions, and its optimum pH is generally 9-11. It is mainly used in the enzyme-added detergent industry. It is also widely used in leather, silk, feed, medicine, food, environmental protection and other industries. [0003] At present, proteolytic enzymes are the most widely used industrial enzymes in the world, accounting for about 60% of the total enzymes, of which alkaline proteases account for 25%. Its huge application prospects in business and its important role in basic research have attracted many companies and research institutes at home and abroad to conduct multi-faceted research on it. [0004] The preliminary research of the laboratory throug...

AI Technical Summary

Problems solved by technology

Fermentation with high nutrient concentration fermentation broth will produce a deterrent effect, inhibit the growth of bacteria, and is also not conducive to enzyme production

At the same time, the nitrogen source in the fermentation medium is cottonseed cake powder, although it is beneficial to enzyme production, but it is a crude material for fermentation, the utilization rate of raw materials is low, and it contains a large number of tiny particles, which brings difficulties to the separation and purification of enzymes in the later stage.

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