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Bacillus alcalophilus strain wp-1 and application thereof

A technology of Bacillus alkalophilus, wp-1, which is applied in the field of agricultural microorganisms and can solve problems such as poor plant growth and soil salinization

Active Publication Date: 2019-12-24
SHIHEZI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the defects of serious soil salinization and poor plant growth in saline-alkali soil, the present invention provides an alkalophilic Bacillus strain wp-1 with salt-tolerant growth-promoting effect, which can improve the stress resistance of plants, secrete Indoleacetic acid, which synthesizes siderophores and promotes plant growth

Method used

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  • Bacillus alcalophilus strain wp-1 and application thereof
  • Bacillus alcalophilus strain wp-1 and application thereof
  • Bacillus alcalophilus strain wp-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1.1 Isolation and purification of strains

[0049] The soil was collected from the saline-alkali land of the 146th regiment of the Eighth Division of Xinjiang, put into a clean sampling bag, marked, and stored in a 4°C refrigerator for later use. Weigh 10g of the collected soil sample and place it in a 250mL Erlenmeyer flask of 90mL sterilized water. Mix thoroughly in the test tube, then use a pipette to draw 1mL from this test tube and add it to another test tube containing 9mL sterile water, mix well, and so on to make 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 After the solutions with different dilutions, draw 0.1mL each and spread evenly on the bacterial culture medium (LB) plate, invert it in a 30°C incubator and cultivate it for 2-3 days, pick a single colony and connect it on the LB slant, and wait for it to grow out. Store in a 4°C refrigerator after the bacterial lawn.

[0050] LB medium: 5g of yeast extract, 10g of tryptone, 10g of sodium chloride, 1...

Embodiment 2

[0055] 2.1 Study on growth characteristics of strain wp-1

[0056] 2.1.1 Effect of different pH on the growth of strain wp-1

[0057]The medium is LB-based medium, and the pH is adjusted to 6, 7, 8, 9, 10, 11, and sterilized at 121°C for 20 minutes when preparing the medium. Cultivate the strain in LB culture medium overnight, inoculate it with 1.0% inoculum amount in LB culture medium with different pH, culture with shaking at 150r / min, 30°C for 24h, and use 722 spectrophotometer to detect the optical density (D 600 nm), the results are shown in Table 1, and the optimum growth pH is 10.

[0058] Table 1 Effects of different pH on the growth of strain wp-1

[0059]

[0060] 2.1.2 Effects of different temperatures on the growth of strain wp-1

[0061] Cultivate the strain in LB culture medium overnight, inoculate the strain in LB culture medium (1% NaCl, pH 8) with 1.0% inoculum, respectively place them in a temperature gradient of 24-36°C, shake at 150r / min for 24h, and...

Embodiment 3

[0069] 3.1 Quantitative determination of phosphorus-dissolving ability of strain wp-1

[0070] Montina organophosphate medium (1L): glucose 10.0g, (NH4) 2 SO 4 0.5 g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 ·H 2 O 0.03g, lecithin 0.2g, CaCO 3 1.0 g, yeast powder 0.5 g, agar 20.0 g; distilled water 1000 mL; liquid medium without agar, sterilized at 121°C for 20 minutes.

[0071] PKO inorganic phosphorus medium (1L): glucose 10.0g, MgSO 4 ·7H 2 O 0.3g, (NH 4 ) 2 SO 4 0.5g, NaCl0.3g, Ca 3 (PO 4 ) 22.0g, KCl 0.3g, MnSO 4 ·H 2 O 0.03g, FeSO 4 ·7H 2 O 0.036g, agar 20g, distilled water 1000mL; liquid medium without agar, sterilized at 121°C for 20 minutes.

[0072] 2μg·mL -1 Gradiently dilute the phosphorus standard solution to the phosphorus content of 0.00, 0.04, 0.08, 0.24, 0.40, 0.80, 1.20μg·mL -1 Make a standard curve. Add molybdenum antimony anti-color developing agent at room temperature, develop color for 20min, measure its...

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Abstract

The invention provides a bacillus alcalophilus strain wp-1 and application thereof, and relates to the technical field of agriculture microbes. The bacillus alcalophilus strain wp-1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2018427. The bacillus basophilus strain wp-1 has the ability to tolerate salt and alkaline environments. The bacillus basophilus strain wp-1 has the ability to convert insoluble phosphate into soluble phosphorus which can be absorbed by plants, can further synthesize indoleacetic acid (IAA) by using tryptophan, and has the ability to synthesize iron carriers. The bacillus basophilus strain wp-1 can decrease the malondialdehyde content in wheat roots, and can be used for improving plant stress resistance. Thebacillus basophilus strain wp-1 further has the effect of promoting the plants to grow in non-stress environments and salt-stress environments.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to an alkalophilic bacillus strain wp-1 and its application. Background technique [0002] Soil salinization is a worldwide problem, involving all regions of the world, especially in arid and semi-arid regions. The global saline soil area is about 1×10 9 hm 2 , has great potential for the development of comprehensive agriculture. my country is one of the countries with large saline-alkali land in the world, and the total area of ​​saline-alkali soil is about 0.99×10 8 hm 2 (Zhu Jianfeng, Cui Zhenrong, Wu Chunhong, Deng Cheng, Chen Junhua, Zhang Huaxin. Research progress and prospect of saline-alkali land greening in my country [J]. World Forestry Research, 2018,31(04):70-75), and widely distributed, from tropical to frigid There are a large number of saline soils in the temperate zone, from the coastal area to the inland area, from the humid area to the extre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12P17/10C12P3/00A01G7/06A01G13/00C12R1/07
CPCA01G7/06A01G13/00A01N63/00C12P3/00C12P17/10C12N1/205C12R2001/07
Inventor 张凤华王丹赵亚光
Owner SHIHEZI UNIVERSITY
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