64 results about "Cell Migration Process" patented technology

The invention relates to conjugates of anti-integrin specific antibodies with cytotoxic compounds, the synthesis, selection, and use of such conjugates for use in cancer therapy or other diseases mediated by cell proliferation, cell migration, or inflammation and which pathology involves angiogenesis or neovascularization of new tissue. In addition the invention relates to combination therapy of such diseases wherein the treatment comprises use of said conjugates in combination with one or more other treatment modalities including but not limited to: chemotherapy, surgery or radiation therapy. The preferred conjugates contain maytansinoid compounds linked to the antibody by a disulfide linkage, and preferred chemotherapeutic agents are doxorubicin, a taxane, a camptothecin, a podophyllotoxin, a nucleoside analog, or a pyrimidine analog.

The invention discloses an imaging method for a bistatic forward-looking synthetic aperture radar (SAR). Aiming at the defect existing when the existing method is used for the imaging process of the bistatic forward-looking synthetic aperture radar, the imaging method adopts a bistatic forward-looking SAR point target response two-dimensional frequency spectrum based on the least square polynomial fitting; the frequency spectrum is the least square approximation of the two-dimensional frequency spectrum with the accurate theory. According to the characteristics of bistatic forward-looking invariant SAR direction, variant range and nonlinear and variant Doppler centroid range of range cell migration in an RD (radar domain) domain, bistatic forward-looking SAR range migration correction, secondary-range compression and high-order phase compensation are realized by the frequency spectrum so as to accurately focus the bistatic forward-looking SAR. Compared with the traditional SAR imaging method and the bistatic forward-looking SAR imaging method, the method disclosed by the invention has higher imaging precision.

Methods and apparatuses involving biocompatible structures for tissue engineering and organ replacement and, more specifically, biocompatible structures formed by three-dimensional fabrication, are described. In some embodiments, the biocompatible structures are scaffolds for cells that can be used as tissue engineering templates and / or as artificial organs. The structures may be three-dimensional and can mimic the shapes and dimensions of tissues and / or organs, including the microarchitecture and porosities of the tissues and organs. Pores in the structure may allow delivery of molecules across the structure, and may facilitate cell migration and / or generation of connective tissue between the structure and its host environment. Structures of the invention can be implanted into a mammal and / or may be used ex vivo as bioartificial assist devices.

The invention provides a frequency domain imaging method for a remote sensing satellite irradiation source forward-looking synthetic aperture radar (LSA-FBSAR). In the method, a nonlinear mapping relationship between range dimension space-variant range cell migration (RCM) and azimuth dimension space-variant RCM is acquired from the complex coupling relationship of a two-dimensional frequency spectrum by the resolution representation of the frequency spectrum of an LSA-FBSAR system and the characteristic of forward-looking work of a receiving platform. The conventional two-dimensional space-variant RCM compensation processing procedure is improved; the compensation of the target azimuth of a non-reference point and slant-range two-dimensional space-variant RCM is realized by scaled Fourier transform-phase multiplication technology and interpolation-phase multiplication technology; the problem of severe geometric distortion such as bending, distortion and the like of an imaging result caused by two-dimensional nonlinear space-variant RCM is effectively solved; and the method is suitable for realizing the high-resolution imaging of the LSA-FBSAR.

The invention provides a novel multi-subarray synthetic aperture sonar fast imaging algorithm. In accordance with a subsystem composed of a single receiving array element and a transmitting array element,two-dimensional frequency domain system function is calculated. Based on the characteristics that the azimuth focusing and the range direction processing are relatively independent, azimuth pulse-compression phase modulation function is calculated. Based on the phases of the two-dimensional frequency domain system function and the azimuth pulse-compression phase modulation function, the distance-azimuth coupling phase term is calculated. The subsystem echo data is made uniform correction process through coupling phase. The range direction data is partitioned. The differential correction of coupling phase is utilized to realize the participant range cell migration correction of the subsystem signal. The subsystem signal is performed azimuth pulse compression. After imaging and processing echo data of the subsystem composed of each receiving array element and the transmitting array element, all of coarse resolution images are performed coherence stack, so as to obtain the final high resolution image of the synthetic aperture sonar. The novel multi-subarray synthetic aperture sonar fast imaging algorithm has the advantages of overcoming the complex design process of the traditional imaging algorithm and improving the imaging performance of the imaging algorithm within the whole swath.

The invention discloses a moving-target parameter estimation method of common-rail bistatic synthetic aperture radar (SAR). The method mainly aims at resolving the problem that moving-target parameter estimation under the Doppler ambiguity condition cannot be achieved in the prior art. The method includes the following steps: (1) carrying out distance compression on radar echo signals of bistatic SAR of different channels; (2) carrying out clutter cancellation on the distance-compressed signals with the data processing control area (DPCA) method, and obtaining sparse moving-target signals; (3) carrying out radon transformation on the sparse moving-target signals, and obtaining a moving-target vertical heading speed and distance position coordinates; (4) carrying out range cell migration correction by means of the moving-target vertical heading speed, meanwhile compensating for the linear phase caused by the range cell migration correction, and eliminating Doppler center frequency ambiguity; and (5) utilizing the optimization method based on the compressed sensing theory to acquire the moving-target heading speed and the position. According to the moving-target parameter estimation method, moving-target parameter estimation of a bistatic SAR system under the low pulse repetition frequency (PRF) condition can be achieved. The method can be used for moving-target imaging and target tracking.

The invention provides a monopulse foresight imaging processing method of a missile-borne / airborne radar and belongs to the field of signal processing. First, focus processing in the range direction is finished through matched filtering processing in the range direction, and separation in the range direction of a ground target is achieved, then, distance cell migration correction processing is finished according to a relative position between a radar platform and a target area, and finally accumulation of azimuth energy is achieved through locating of the azimuth direction by using monopulse angle measurement technology, and a final imaging processing result is obtained. The processing method aims at removing a relative motion phenomenon existing between the radar carrier platform and the ground, and the relative motion between a radar carrier platform and the ground target is corrected through motion compensation processing, so that effects of the relative motion on the angle measurement are reduced, and difficulty of following locating is relieved. Pulse pressure processing in the range direction and a range cell migration correction angle measurement method are combined, and precision of the angle measurement is improved.

The present invention generally relates to pharmacology and, in particular, to the treatment of tumors and other conditions. In some aspects, the invention is directed to the treatment of subjects having tumors or cancers that are metastatic. Surprisingly, it has been found that certain compositions comprising oxidized glutathione-based compounds are able to effectively treat such cancers by inhibiting cell migration and / or invasion processes, and thus, inhibiting tumor cell metastases. Without being bound by any theory, it is believed that such compositions are effective since the compositions are able to suppress the activation of critical signaling pathways within cells that are used for cell migration, such as the ErbB2 and / or phosphoinositide-3 kinase (PI3K) pathways, including the downstream RhoA and AKT pathways. Such pathways are regulated by ERp5, which is a protein disulfide isomerase regulated using certain redox pathways, and those redox pathways are unusually sensitive to treatment using oxidized glutathione-based compounds. Thus, the composition of the instant invention, in some embodiments, are surprisingly effective at preventing tumor metastases. While other references have disclosed the treatment of cancers using oxidized glutathione-based compounds, no reference has suggested that signaling pathways used for cell migration, invasion and metastasis, such as the ErbB2, PI3K, RhoA, and AKT pathways, are highly susceptible to treatment by altering the redox state of the cell, e.g., by oxidized glutathione-based compounds. Accordingly, the use of such compositions to treat tumor metastases is surprising and could not be predicted given the teachings of the prior art.

The invention provides an establishment method, an establishment device, and a manufacturing system for a 3D porous support model, and relates to the technical field of medical science. The establishment method for a 3D porous support model comprises: according to CT image data of skeleton lesion or defect positions, obtaining outline data of a to-be-established support and bone mineral density distribution of each corresponding pixel point of the to-be-established support in the CT image data, selecting an element of a topological structure type in minimum structure, and based on the selectedtopological type structure element and the outline data, generating an uniform porosity initial support model, using the corresponding bone mineral density distribution of each pixel point, adjustingthe porosity of each structure element and micropore size in the initial support model, to generate a 3D porous support model like cancellous bone corresponding to the to-be-established support. A porous support printed according to the 3D porous support model like cancellous bone satisfies a bionic microstructure having biomechanical characteristics on bone defect positions, and satisfies cell migration, differentiation, and multiplication and other cell growth microenvironment.

The invention relates to a cell migration high-resolution impedance real-time tracking, measurement and control device, and a manufacturing method and an application method thereof, and is particularly suitable for cell impedance measurement, cell migration, cell detection, medicine screening, microfluidics and a nano technology. The tracking, measurement and control device comprise a cavity; a chamber in which liquid is accommodated is formed in the middle of the cavity; the middle of the chamber is divided into an upper chamber and a lower chamber by a microporous membrane; an upper chamber electrode is inserted into the electrode hole of an upper cavity; a lower chamber electrode is inserted into the electrode hole of a lower cavity; the two electrodes are connected with an analysis device respectively; and the lower part of the lower cavity is provided with a liquid inlet and outlet channel which is communicated with the external. The invention has the advantages that: 1, cells are detected in real time, and a transmembrane migration process of cells cultured in vitro is reliably tracked, measured, controlled and analyzed in real time at high resolution in a non-destructive way; 2, detection sensitivity is improved and the transmembrane migration of a single cell is tracked; 3, the effect of a medicine on the cell can be precisely determined through a microflow pipeline; 4, manual measurement and control errors are reduced; 5, the tracking, measurement and control device is easy and convenient to operate and high in accuracy; and 6, the tracking, measurement and control device is easy to manufacture and low in cost.

The invention discloses a bistatic forward-looking SAR non-stationary clutter suppression method based on cascade cancellation, and is applied to the field of clutter suppression in moving target detection of synthetic aperture radars. The method aims at solving the problem that clutter suppression performance is seriously reduced because a traditional space-time self-adaptive method cannot accurately estimate a covariance matrix of clutter due to a strong non-stationary characteristic of bistatic clutter in the prior bistatic forward-looking SAR, and comprises the following steps that a de-skew pre-filter and Keystone transformation are firstly adopted to respectively remove Doppler ambiguity and correcting range cell migration; time division STAP echo domain processing is then adopted topreliminarily inhibit bistatic forward looking SAR non-stationary clutter, and a signal-to-noise ratio is improved; and DPCA image domain processing based on BP imaging is finally adopted, a clutterunit is reduced, residual non-stationary clutter is fully inhibited, and the signal-to-clutter ratio is further improved, thereby achieving the detection of a moving target.

The invention discloses a nonlevel flight double-station SAR frequency domain FENLCS imaging method based on a quadratic elliptic model. The method comprises the following steps: firstly, performing range direction pretreatment, namely applying linear range walk correction, KT (keystone transform), bulk range cell migration correction and second range compression in a range direction; secondly, executing residual high-order range cell migration correction treatment of azimuth space variation. According to a range direction pretreatment result, the quadratic elliptic model is put forward in theinvention, and the quadratic elliptic model is used for accurately describing azimuth space variation characteristics of an equidistant point receiver squint angle and beam center slope distance. Theimproved FENLCS imaging processing method based on the quadratic elliptic model, provided by the invention, realizes removal of characteristics of space variation with azimuth positions of a residualdoppler center, doppler modulation frequency and a high-order phase coefficient, and consequently completes azimuth unified focusing treatment. Additionally, a frequency domain high-order non-space variation prefiltering method is provided, a derivation procedure of a FENLCS algorithm is simplified, and focusing quality is improved.

The invention belongs to the technical field of radar, and discloses a ground accelerating moving target imaging method based on a quadratic fit range equation. The method is used for imaging a ground accelerating moving target through synthetic aperture radar. The method includes the steps that (1) quadratic fit is carried out on a target range equation, (2) an original echo signal of the target is changed into a two-dimensional frequency domain, (3) according to the fitted secondary range equation, a range matching filter is built in the two-dimensional frequency domain and range compression and range cell migration correction are carried out, (4) the signal after range compression and range cell migration correction is changed into a range Doppler domain, (5) an azimuth matching filter is built in the range Doppler domain and direction compression is carried out, (6) azimuth inverse discrete fourier transform is carried out on the signal after direction compression, and imaging of the target is finished. Precise imaging of the ground accelerating moving target can be achieved without the need of knowing speed parameters, position parameters and the acceleration speed of the target, and calculation efficiency is high.

The invention discloses a high-resolution wide-width satellite-borne mosaic SAR imaging processing method and system, wherein the method provides and analyzes the azimuth signal expansion problem after Range Cell Migration Correction (RCMC) is carried out by using a high-precision Range Cell Migration Correction Algorithm (RMA) under a large squint angle, then changes of Doppler central frequencyalong with range frequency, equivalent speed two-dimensional space variation and high-squint high-precision RCMC are solved by an imaging processing mode of azimuth molecular aperture up-sampling + azimuth signal expansion + RMA-RCMC + azimuth scale scaling + azimuth SPECAN, and high-resolution wide-width mosaic SAR high-precision imaging is realized. Compared with other algorithms such as a two-step algorithm and the like, the high-resolution wide-width satellite-borne mosaic SAR imaging processing method has the advantages that the obtained image sampling unit is more equivalent to the resolution magnitude, the data processing efficiency is higher, and the limitation of processing high-resolution wide-width mosaics by a sub-aperture method is effectively solved. Meanwhile, the high-resolution wide-width satellite-borne mosaic SAR imaging processing method is high in algorithm precision, stable and strong in practicability.

A device for studying cell migration and deformation, a method of manufacturing and using the device. The device includes at least two channels defined therein. The two channels are separated by a partitioning wall therebetween. Each channel has an inlet and an outlet. At least one through passage is defined in the partitioning wall to allow fluid communication between the two channels. The method of manufacturing includes molding a substrate having the channels and passage defined therein and covering the substrate with a cover.

The invention relates to a fibroin / nylon composite material and a preparing method thereof. The preparing method comprises the steps of conducting degumming treatment on silk to obtain silk fibroin fiber; dispersing the silk fibroin fiber into mixed liquor of calcium salt / formic acid to obtain silk fibroin fiber dispersion liquid; dropwise adding the silk fibroin fiber dispersion liquid into a carbonate solution to obtain reaction liquid; then filtering the reaction liquid, and conducting washing and drying on a filter cake to obtain calcium carbonate nanowire; dissolving nylon into the silk fibroin fiber dispersion liquid and then adding the calcium carbonate nanowire into the silk fibroin fiber dispersion liquid to obtain a blended solution; adopting a tape-casting method to conduct film-spreading on the blended solution, and conducting drying to obtain dry film; putting the dry film into deionized water to be subjected to washing, and conducting infrared drying at the temperature of 60-60 DEG C to obtain the fibroin / nylon composite material. The internal structure of the obtained fibroin / nylon composite material is mainly composed of fiber, and is composed of the calcium carbonate nanowire at the same time, and thus the fibroin / nylon composite material has a good mechanical property, is very beneficial to transportation of nutrient substances, cell migration, and the growth of tissues, and therefore the fibroin / nylon composite material is an ideal regeneration medical material.

The invention discloses a synthetic aperture radar (SAR) echo data range cell migration (RCM) correction method and device. The method includes the following steps that: range compression and geometric RCM correction are performed on echo data, so that post-range compression time-domain data are obtained; sub aperture segmentation is performed on the post-range compression time-domain data, so that sub aperture data can be obtained, and sub block data with a highest signal-to-noise ratio (SCR) in the sub aperture data are selected; the residual RCM of the sub aperture data are determined according to the selected sub block data; full aperture combination is performed on the residual RCM of the sub aperture data, so that the full-aperture combination of the residual RCM can be obtained; the residual RCM of the echo data is corrected according to the full-aperture combination of the residual RCM; and the echo data of which the residual RCM has been corrected are adopted to perform imaging. The invention also discloses a synthetic aperture radar (SAR) echo data range cell migration (RCM) correction device.

Disclosed is a root canal filler for non-extracted tooth which causes no internal resorption or external resorption in a tooth with complete root formation, shows no odontoclast, and contributes to the regeneration of a dental tissue in which odontoblasts are smoothly aligned on the dentin wall. After pulpectomy or enlargement / cleaning of an infected root canal, a root canal filler for non-extracted tooth, which comprises tooth pulp stem cells and an extracellular matrix, is inserted into the apical side of the root canal of the non-extracted tooth. The tooth pulp stem cells may be, for example, dental pulp CXCR4-positive cells. It is preferred to attach, to the crown side of the root canal, migration factor(s) including at least one factor selected from among a cell migration factor, a cell proliferation factor, a neurotrophic factor and an angiogenic factor.

The invention discloses a conductive microneedle paster for wound repairing and a preparation method of the conductive microneedle paster. According to the method, through multi-template copying and solidification at different steps, conductive microneedle units which are provided with inclined needle tips and are wrapped by "E-shaped" guide tracks are prepared, two conductive microneedle units with needle tips opposite to each other are connected with a gauze at an interval, and are respectively connected with a power supply anode and a power supply cathode through leads fixed in the "E-shaped" guide tracks, and then the buckle-shaped conductive microneedle paster is prepared. The conductive microneedle paster disclosed by the invention can be adhered to a wound, the buckle-shaped needletips are beneficial to shrinkage and fixation of the wound, when an external power supply is connected, a spatial electric field can be formed between two microneedle units, cell migration can be induced, cell proliferation and differentiation can be promoted, wound healing can be accelerated, and the conductive microneedle paster is diverse in function, remarkable in treatment effect and wide inapplicable range.

Systems, including methods, apparatus, compositions, and kits, for multiplexed assay of cell migration with subdivided cell holders and / or microplates.

The invention provides reptile antibacterial peptide OH-CATH30 nanometer granules and nanofibers and an application of the nanofibers. The prepared nanometer granules and the nanofibers can realize secondary encapsulating of Cathelicidin antibacterial peptide, so that rapid degradation of antibacterial peptide by cells and in vivo enzymes can be effectively avoided, and the biological function canbe exerted for a long time. According to the nanometer granules disclosed by the invention, a static electricity liquid drop preparation method is adopted, and the nanofibers adopt a high-pressure electrostatic spinning method. The preparation method is simple and easy to operate, and is high in entrapment efficiency. The prepared nanometer granules and the prepared nanofibers both have notable antibacterial activity, besides, have notable effects on promotion of cell migration and wound heal, and can be used for preparing biology biodegradable wound dressing for resisting bacterium infectionand promoting wound healing.

Low-cost and portable microfluidic systems are needed for cell migration research and Point of Care (POC) testing. This study introduces a low-cost and portable USB Microscope Microfluidic Chemotaxis Analysis System (UMCAS) for rapid analysis of cell chemotaxis studies. A standalone microfluidic gradient generator is also developed for rapid generation of chemical gradient in microfluidic device without need of any peripheral perfusion apparatus. A smart phone based application program was developed for the real-time remote monitoring of the migration data. This system is validated by observing the neutrophil migration in three different conditions: 1) medium control, 2) uniform IL-8 control, and 3) IL-8 gradient. The results show that neutrophils exhibit random migration in both medium and uniform IL-8 control experiments, while they show strong directional migration to the IL-8 gradient. These results successfully validated the developed UMCAS system.

The embodiment of the invention relates to the technical field of communication. The invention particularly relates to a cell configuration method and device. The objective of the invention is to reduce signaling interaction in a cell migration process and improve cell migration efficiency. The method comprises the steps that a first management function unit acquires a first message, wherein the first message comprises information of a first cell and information of a first base station, the information of the first cell comprises at least one of an identifier of the first cell or a managementobject identifier of the first cell, and the information of the first base station comprises at least one of an identifier of the first base station or a management object identifier of the first basestation; the first management function unit configures the relationship between the first cell and the first base station according to the first message; or, adjusts configuration information relatedto the first cell, wherein the step of adjusting the configuration information related to the first cell comprises: adjusting a neighbor cell relationship associated with the first cell, or adjustingan external cell associated with the first cell, wherein the first cell is associated with the second base station before being associated with the first base station.

The invention discloses a scanning synthetic aperture radar (ScanSAR) image quality improving method; the method comprises the following steps: applying range direction compression and range cell migration correction (RCMC) to ScanSAR data of each mapping sub-band; applying sub-aperture division to the data after being applied with the range direction compression and the RCMC; respectively applying aperture interpolation to each sub-aperture data; applying azimuth compression to the sub-aperture data after being applied with the interpolation. The invention further discloses a scanning synthetic aperture radar (ScanSAR) image quality improving device.

The present invention is directed to creating a highly versatile, high throughput, and convenient platform for interrogating migration phenotypes of GB cells in the context of diverse environmental parameters. Specifically, engineered substrates are employed to mimic the mechanical signals of natural ECM topography, and combine these tools with chemical cues presented in soluble and immobilized forms (PDGF and laminin, respectively). This platform achieves far greater resolution and sensitivity in migration analyses than do commonly used methods. More importantly, it can provide highly informative, patient specific results regarding tumor progression in vivo. Furthermore these capabilities strongly convey the immense clinical, prognostic potential of this simple test.

Lipid multilayer structures are formed by evaporating a solvent from each of a plurality of lipid solutions thereby form the lipid multilayer structures. Each lipid solution comprises the solvent and one or more lipids. Each lipid multilayer structure is a microstructure comprising one or more lipids.

The invention relates to a shRNA (short hairpin ribonucleic acid) targeted interfering YB-1 gene human lung adenocarcinoma A549 cell strain A549 / YBX1-homo-746 capable of stably expressing GFP (green fluorescent protein) and a shRNA (short hairpin ribonucleic acid) targeted interfering YB-1 gene human lung adenocarcinoma A549 cell strain A549 / YBX1-homo-326 capable of stably expressing GFP, which are prepared by the following steps: constructing a targeted interfering YB-1 gene shRNA eukaryon expression vector, transfecting a human lung adenocarcinoma A549, and carrying out G418 screening. The collection numbers are respectively CCTCC C201522 and CCTCC C201523. The cell strains provide convenience for later research in correlation between in Yb-1 gene and cytobiological behavior change of lung adenocarcinoma, effectively shorten the research period and enhance the research result stability. The cell strains can stably express GFP, thereby providing convenience for later research in cell migration and invasion capacities and other cytobiological functions.

The invention relates to a microfluidic chip applicable to research on cell migration. The microfluidic chip comprises a microfluidic layer, a control layer and a connecting layer, wherein the connecting layer is used for connecting the microfluidic layer and the control layer; the microfluidic layer is provided with at least one microfluidic channel; the inner surface of the microfluidic channel consists of a material suitable for growth of cells; the control layer is provided with at least one control channel; the cell growth microfluidic channel and the at least one control channel is provided with at least one intersected point; the connecting layer at the position of the intersected point consists of an elastomer material; when pressure in the control channel is increased to a certain value, the elastomer connecting layer at the position of the intersected point is expanded towards the direction of the microfluidic layer and is contacted with the inner surface of the microfluidic channel to form a contact surface with certain size; and the width of the contact surface is less than that of the microfluidic channel at the position of the intersected point, so that the microfluidic channel is in the communication state. The microfluidic channel is simple to prepare and convenient to use and does not damage to the cells in the process of researching on the cell migration.