Cell migration high-resolution impedance real-time tracking, measurement and control device, and manufacturing method and application method thereof

A high-resolution, real-time tracking technology, applied in material impedance, analytical materials, instruments, etc., to achieve high-throughput drug screening and disease diagnosis, improve detection sensitivity, and quantify human errors.

Active Publication Date: 2012-07-04
武汉介观生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can also monitor cell transmembrane migration in real time, its resolution is very limited, and the drug delivery and drug-induced time response are not optimized.

Method used

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  • Cell migration high-resolution impedance real-time tracking, measurement and control device, and manufacturing method and application method thereof
  • Cell migration high-resolution impedance real-time tracking, measurement and control device, and manufacturing method and application method thereof
  • Cell migration high-resolution impedance real-time tracking, measurement and control device, and manufacturing method and application method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Example 1: It is a basic embodiment of the cell migration high-resolution impedance real-time tracking measurement and control device in which the cavity 1 is a combined structure of the present invention. figure 1 is an example of this. It includes a chamber 1, a chamber 2 that can hold liquid in the middle of the chamber 1, and a microporous membrane 3 in the middle of the chamber 2 to separate the chamber 2 into an upper chamber 2.1 and a lower chamber 2.2, and the microporous The upper part of the film 3 is the upper cavity 1.1, and the lower part is the lower cavity 1.2; the lower part of the upper cavity 1.1 and the upper part of the lower cavity 1.2 are respectively provided with electrode holes 6 connected from the outside to the inner cavity near the microporous film 3; The electrode hole 6 of the chamber body 1.1 is inserted into the upper chamber electrode 6.1, and the electrode hole 6 of the lower chamber body 1.2 is inserted into the lower chamber electr...

Embodiment 2

[0049] Example 2: Such as figure 1 , 2 Shown is an embodiment of the cell migration high-resolution impedance real-time tracking measurement and control device in which the cavity 1 is a composite structure of the present invention. The cavity 1 is a combined structure, that is, the upper cavity 1.1, the lower cavity 1.2 and the base 1.3 closing the bottom of the lower cavity 1.2 are three independent components; the upper cavity 2.1 and the lower cavity 2.2 are processed once or separately The microporous film 3 is sandwiched between the lower end surface of the upper cavity 1.1 and the upper end surface of the lower cavity 1.2; the upper cavity 1.1 and the lower cavity 1.2 are sealed and connected; The base 1.3 is hermetically connected. The material of the upper cavity 1.1 and the lower cavity 1.2 is a polymer material used in the field of microfluidics, which is selected from PDMS, that is, polydimethylsiloxane, or thermoplastic material polymethyl methacrylate PMMA ...

Embodiment 3

[0050] Example 3: Different from the above-mentioned embodiment 2, as image 3 , 4 Shown is an embodiment of the cell migration high-resolution impedance real-time tracking measurement and control device in which the cavity 1 is an integral structure of the present invention. The cavity 1 is an integral structure, that is, the upper cavity 1.1, the lower cavity 1.2 and the base 1.3 that closes the lower end of the lower cavity 1.2 are an integral component; the aperture of the lower chamber 2.2 is smaller than the aperture of the upper chamber 2.1, and the microporous film 3 is set on the microporous film ring 4 and embedded in the upper chamber 2.1. The upper cavity 1.1, the lower cavity 1.2 and the base 1.3 of the cavity 1 of the overall structure are processed from an integral material. The material is a polymer material used in the field of microfluidics, which is selected from PDMS, that is, polydimethylsiloxane Methyl siloxane, or thermoplastic material polymethyl me...

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Abstract

The invention relates to a cell migration high-resolution impedance real-time tracking, measurement and control device, and a manufacturing method and an application method thereof, and is particularly suitable for cell impedance measurement, cell migration, cell detection, medicine screening, microfluidics and a nano technology. The tracking, measurement and control device comprise a cavity; a chamber in which liquid is accommodated is formed in the middle of the cavity; the middle of the chamber is divided into an upper chamber and a lower chamber by a microporous membrane; an upper chamber electrode is inserted into the electrode hole of an upper cavity; a lower chamber electrode is inserted into the electrode hole of a lower cavity; the two electrodes are connected with an analysis device respectively; and the lower part of the lower cavity is provided with a liquid inlet and outlet channel which is communicated with the external. The invention has the advantages that: 1, cells are detected in real time, and a transmembrane migration process of cells cultured in vitro is reliably tracked, measured, controlled and analyzed in real time at high resolution in a non-destructive way; 2, detection sensitivity is improved and the transmembrane migration of a single cell is tracked; 3, the effect of a medicine on the cell can be precisely determined through a microflow pipeline; 4, manual measurement and control errors are reduced; 5, the tracking, measurement and control device is easy and convenient to operate and high in accuracy; and 6, the tracking, measurement and control device is easy to manufacture and low in cost.

Description

Technical field: [0001] The invention relates to a cell migration high-resolution impedance real-time tracking measurement and control device and its manufacturing method and application method, which is especially suitable for cell electrical impedance measurement, cell migration, cancer detection, drug screening, microfluidic control and nanotechnology applications, and belongs to biological detection technology field. Background technique: [0002] Cell electrical impedance sensing technology is a technology that measures changes in the electrical impedance of a detection system due to changes in cell morphology, cell movement, or mutual contact between cells. This technology has the advantages of long-term, real-time, fast, non-destructive, and simple analysis of in vitro cultured cells. It has been widely used in the fields of cell morphology change tracking, drug screening, biochemical detection, and disease diagnosis. It has become the first choice for biosensor res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/02G01N35/00
Inventor 江莲梅刘剑淼汪莉石剑陈勇
Owner 武汉介观生物科技有限责任公司
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