Reptile antibacterial peptide OH-CATH30 nanometer granules and nanofibers and application of nanofibers

A technology of OH-CATH30 and reptiles, which is applied in the field of biomedicine, can solve problems such as medical problems where no treatment has been found, and achieve significant anti-infection effects, good inhibitory effects, and significant antibacterial effects

Inactive Publication Date: 2019-06-07
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The healing and repair of skin wounds, as well as the formation of scars, is still a major medical problem that researchers and patients in th

Method used

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  • Reptile antibacterial peptide OH-CATH30 nanometer granules and nanofibers and application of nanofibers
  • Reptile antibacterial peptide OH-CATH30 nanometer granules and nanofibers and application of nanofibers
  • Reptile antibacterial peptide OH-CATH30 nanometer granules and nanofibers and application of nanofibers

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Experimental program
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Embodiment 1

[0032] Example 1, Preparation of Reptile Cathelicidin Antimicrobial Peptide Nanoparticles and Detection of Encapsulation Efficiency and Slow Release

[0033] 1. Preparation of Reptile Cathelicidin Antimicrobial Peptide Nanoparticles

[0034] The preparation of reptile cathelicidin antimicrobial peptide nanoparticles comprises the following steps:

[0035] (1) Prepare a carboxymethyl chitosan (CMCS) solution with a concentration of 1 mg / mL and an antimicrobial peptide OH-CATH30 solution with a concentration of 1 mg / mL with deionized water, respectively, and filter them with a 0.22um microporous membrane until they are well bacteria in the EP tube.

[0036] The antimicrobial peptide OH-CATH30 solution used in the present invention is referred to as OH-30 solution for short, and the sequence of the antimicrobial peptide OH-30 is: KFFKKLKNSVKKRAKKFFKKPRVIGVSIPF (SEQ ID No: 1).

[0037] (2) Transfer the above-mentioned certain amount of CMCS solution to a sterilized container wit...

Embodiment 2

[0044] Embodiment 2, the preparation of reptile cathelicidin antimicrobial peptide nanofiber

[0045] The preparation method of reptile cathelicidin antimicrobial peptide nanofiber is as follows:

[0046] (1) Dissolve 2% (w / v) chitosan (CS) (0.1 g) in 1 mL of 15% (v / v) acetic acid solution and stir until dissolved.

[0047] (2) 9% (w / v) polyvinyl alcohol (PVA) (1.8 g) was dissolved in 8.4 mL of deionized water and stirred at constant temperature at 95 °C for 1.5 h.

[0048] (3) Add 1.4mL DMSO, 0.1mL glycerin and 0.1mL Tween 80 to the above PVA solution, continue to stir at 80°C for 1 hour and then cool to room temperature.

[0049] (4) Add the Cathelicidin antimicrobial peptide nanoparticles prepared in Example 1 (nanoparticle concentration is 0.1-0.3% (w / v)) into the CS solution, mix well, add to the PVA solution and stir magnetically. After continuing the magnetic stirring for 1 hour, the mixture was left standing to remove air bubbles.

[0050] (5) Finally, the above sol...

Embodiment 3

[0052] Embodiment 3, the effect of reptile cathelicidin antimicrobial peptide OH-CATH30 nanoparticles on different bacteria

[0053] Cultivate the strain to be tested overnight in LB medium at 37°C, inoculate it into fresh LB medium the next day, and continue culturing at 37°C until the logarithmic growth phase (OD 600 about 0.9-1), and then diluted with fresh LB medium to a concentration of about 10 6 cfu / ml, spare. Add 100ul of the diluted bacterial solution obtained above to a new 96-well plate, and then add the pre-prepared antimicrobial peptide OH-CATH30 nanoparticles (the amount of nanoparticles added is pre-calculated according to the encapsulation efficiency , so that the final concentration is 100g / ml) was mixed evenly with the bacterial solution, and a blank control group was set. Incubate at 37°C for 12-14 hours, detect OD with a microplate reader 600 , to calculate the inhibition rate. Each experiment was repeated three times, and the results are shown in ima...

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Abstract

The invention provides reptile antibacterial peptide OH-CATH30 nanometer granules and nanofibers and an application of the nanofibers. The prepared nanometer granules and the nanofibers can realize secondary encapsulating of Cathelicidin antibacterial peptide, so that rapid degradation of antibacterial peptide by cells and in vivo enzymes can be effectively avoided, and the biological function canbe exerted for a long time. According to the nanometer granules disclosed by the invention, a static electricity liquid drop preparation method is adopted, and the nanofibers adopt a high-pressure electrostatic spinning method. The preparation method is simple and easy to operate, and is high in entrapment efficiency. The prepared nanometer granules and the prepared nanofibers both have notable antibacterial activity, besides, have notable effects on promotion of cell migration and wound heal, and can be used for preparing biology biodegradable wound dressing for resisting bacterium infectionand promoting wound healing.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to reptile antibacterial peptide OH-CATH30 nanoparticle and nanofiber and application thereof. Background technique [0002] technology [0003] As the outermost protective tissue of the human body, the skin is also the largest organ of the human body. It is often damaged by the outside world and causes skin damage. The healing and repair of skin wounds, as well as the formation of scars, is still a major medical problem that researchers and patients in the medical field must face, but there is still no effective and economical treatment to solve the problem. Solve this major medical problem. [0004] Carboxymethyl chitosan is a chitosan derivative obtained by carboxymethylation of chitosan. It is an amphoteric polyelectrolyte with excellent film-forming properties, water solubility, moisture retention, and biocompatibility. It has the characteristics of good sex and biodeg...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K9/70A61K47/36A61K38/17A61P31/04A61P17/02
Inventor 高媛媛孙同毅李文辉邹鹏飞
Owner WEIFANG MEDICAL UNIV
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