The invention discloses a nanoemulsion of N-[4-(2,4 dimethoxy phenyl)-5-oxygroup-4,5-dihydro-[1,2] disulfide group-[4,3-b]-6-pyrryl]-3,5-di-trifluoromethyl benzamide and a preparation method thereof. The nanoemulsion is prepared from medicines, a water phase, an oil phase, an emulsifier, an assistant emulsifier, and the like and can enhance the dispersity of the medicines and promote the absorption of the medicines so as to further enhance the bioavailability of the medicines. The nanoemulsion has low toxicity, no hemolysis, and the like, is beneficial to the absorption of the medicines and has the advantages of simple and easy preparation method, safe and non-toxic operation, low preparation cost, high medicine content and no medicine loss. The invention is used for preparing the nanoemulsion of the N-[4-(2,4 dimethoxy phenyl)-5-oxygroup-4,5-dihydro-[1,2] disulfide group-[4,3-b]-6-pyrryl]-3,5-di-trifluoromethyl benzamide.