The invention relates to the field of biomedicine, in particular to a kit for predicting the outcome of sperm extraction for patients with non-obstructive azoospermia. The kit of the present invention includes a reagent for detecting the expression level of lncRNA in the sample, the lncRNA includes SPATA42, CCDC37-DT, GABRG3-AS1, LOC440934, LOC100505685, LOC101929088 (XR_927561.2), LOC101929088 (XR_001745218.1), LINC00343, LINC00343, LINC00343, LINC00343 at least one of them. The invention can realize the non-invasive and accurate prediction of the sperm retrieval outcome of NOA patients before operation, and has sufficient scientificity and practicability.