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Application of mercury and cadmium mixed exposure detection in auxiliary diagnosis of non-obstructive azoospermia

A technology for azoospermia and auxiliary diagnosis, applied in the fields of analytical chemistry and clinical medicine, can solve problems such as lack of corresponding attention, and achieve the effect of delaying and preventing disease progression, improving sensitivity and specificity, and easy detection.

Pending Publication Date: 2019-01-22
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of ICP-MS to analyze the mixed exposure levels of mercury and cadmium in male whole blood in the auxiliary diagnosis of non-obstructive azoospermia has not received corresponding attention

Method used

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  • Application of mercury and cadmium mixed exposure detection in auxiliary diagnosis of non-obstructive azoospermia
  • Application of mercury and cadmium mixed exposure detection in auxiliary diagnosis of non-obstructive azoospermia
  • Application of mercury and cadmium mixed exposure detection in auxiliary diagnosis of non-obstructive azoospermia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1 Research Object Selection and Grouping Basis

[0086] The inventor collected whole blood samples from non-obstructive azoospermia cases and normal control males who met the requirements from Nanjing Maternal and Child Health Hospital affiliated to Nanjing Medical University. Those with normal semen examination and fertility were regarded as controls, and those with non-obstructive azoospermia were regarded as cases. A total of 100 cases were randomly enrolled, and 100 control males were matched according to age, BMI, and abstinence time. A total of 200 people were used as experimental subjects for the mixed exposure assessment of mercury and cadmium in non-obstructive azoospermia. The specific sample classification criteria are as follows:

[0087] The first stage of association exploration

[0088] 100 male cases were included, and 100 matched controls were matched according to age, BMI, and abstinence time, for a total of 200 people.

[0089] Group A: ...

Embodiment 2

[0103] Example 2 Analysis of ICP-MS detection of mixed exposure to mercury and cadmium and occurrence of non-obstructive azoospermia

[0104] 1. Sample pretreatment

[0105] Take 100 μl of whole blood, add 4.9ml of reagent A (that is, dilute 50 times), and mix well.

[0106] 2. Instrument testing

[0107] Analytical instrument: ICAP RQ ICP-MS (Thermo)

[0108] a. After ICP-MS is ignited and started, it is stable for about half an hour; pump 1 ppb tuning solution (provided by the instrument manufacturer, Ba, Bi, Ce, Co, Ln, Li, U, each concentration is 1 μg / L, and the matrix is ​​2.5 %HNO 3 , 0.5% HCl), according to the sensitivity of Li, In and U, the oxide (CeO) and the double charge (Ba 2+ ) level, tune the entire mass range (4 ~ 290amu) and achieve the best state; tuning requirements meet the following standards: standard mode sensitivity 7Li≥4000cps / ppb, 115In≥15000cps / ppb; 238U≥20000cps / ppb; standard mode Double charged Ba 2+ / Ba<3.0%; standard mode oxide 156CeO / 140...

Embodiment 3

[0123] Example 3 Production of a kit for detecting mixed exposure of mercury and cadmium in male whole blood for auxiliary diagnosis of non-obstructive azoospermia

[0124] Firstly, the ICP-MS method was used to determine the high levels of mercury and cadmium concentrations in the whole blood of the case group, and then the Chi-square test was used to find that there were significant differences in the composition ratios of individuals with different exposure levels between the healthy control group and the case group. The number of people with high exposure was more than that of the control group, and high exposure to mercury and cadmium significantly increased the incidence of non-obstructive azoospermia. Detection of mixed exposure to mercury and cadmium can be used for auxiliary diagnosis of non-obstructive azoospermia. This kit includes a batch of reagents and consumables for the detection of mercury and cadmium in whole blood. The diluent is used for sample dilution, wh...

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Abstract

The invention belongs to the field of analytical chemistry and clinical medicine, and discloses application of mercury and cadmium mixed exposure detection in auxiliary diagnosis of non-obstructive azoospermia. An exposed biomarker related to the non-obstructive azoospermia is mercury and cadmium mixed exposure. The concentration of mercury and cadmium in whole blood is detected by ICP-MS detection, the application can be used for auxiliary diagnosis of the non-obstructive azoospermia, a few amount of blood is used, and the application is simple, rapid and accurate to operate and has relatively good clinical promotion value.

Description

field of invention [0001] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to an exposure biomarker related to non-obstructive azoospermia and its detection method and application. The exposure biomarker is mercury and cadmium mixed exposure, and the detection method is based on ICP - MS detection method. Background technique [0002] About 30%-55% of human infertility is related to male factor [1] , the most common cause of male infertility is spermatogenesis disorder, clinically known as asthenozoospermia or azoospermia [2] . 10-15% of these are attributed to azoospermia [1] . In recent years, with the continuous improvement of people's reproductive health needs, male infertility has increasingly become the focus of social attention. The research of the WHO Infertility Prevention and Treatment Task Force has shown that reproductive endocrine dysfunction, especially low semen quality, is an important cause of male infertilit...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 夏彦恺杨静张明智卢婷管权权陈敏健王心如
Owner NANJING MEDICAL UNIV
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