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Seminal plasma exosome tsRNA markers related to non-obstructive azoospermia diagnosis and application of seminal plasma exosome tsRNA markers

An azoospermia and marker technology, applied in DNA/RNA fragmentation, recombinant DNA technology, microbial determination/examination, etc., can solve problems such as inability to effectively diagnose non-obstructive azoospermia, and achieve excellent results

Active Publication Date: 2021-01-22
江苏阔然生物医药科技有限公司
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  • Abstract
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Problems solved by technology

[0005] One of the purposes of the present invention is to overcome the deficiency that the existing non-invasive diagnostic indicators cannot effectively diagnose non-obstructive azoospermia, and provide a seminal plasma exosome tsRNA marker related to the diagnosis of non-obstructive azoospermia

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  • Seminal plasma exosome tsRNA markers related to non-obstructive azoospermia diagnosis and application of seminal plasma exosome tsRNA markers
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  • Seminal plasma exosome tsRNA markers related to non-obstructive azoospermia diagnosis and application of seminal plasma exosome tsRNA markers

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Embodiment Construction

[0027] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0028] like figure 1 As shown: the present invention first analyzes the differential tsRNA in seminal plasma exosomes of non-obstructive azoospermia, obstructive azoospermia and healthy control population, and then analyzes the selected differential tsRNA in more non-obstructive azoospermia, Perform real-time fluorescent quantitative PCR verification in samples from patients with obstructive azoospermia, severe spermatogenic dysfunction and healthy controls, and finally determine whether the screened tsRNA is present in testicular tissue samples from patients with obstructive azoospermia and non-obstructive azoospermia Whether there is a difference in expression, to further determine its feasibility as a diagnosis of non-obstructive azoospermia, and conduct bioinformatics functional analysis to determine its possible regulatory mechan...

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Abstract

The invention discloses seminal plasma exosome tsRNA markers related to non-obstructive azoospermia diagnosis and application of the seminal plasma exosome tsRNA markers. The markers are tRF-Pro-AGG-003 and tRF-Val-AAC-010, the sequence of tRF-Pro-AGG-003 is SEQ ID NO.1, and the sequence of tRF-Val-AAC-010 is SEQ ID NO.2. The markers have specificity and sensitivity on non-obstructive azoospermiaand can be used for preparing a diagnostic kit for non-obstructive azoospermia. The two seminal plasma exosome markers tRF-Pro-AGG-003 and tRF-Val-AAC-010 are differentially expressed in seminal plasma of patients suffering from non-obstructive azoospermia and obstructive azoospermia, non-obstructive azoospermia can be well diagnosed, an azoospermia sperm taking result can be predicted, and the effect of the markers is superior to the indexes such as the common plasma follicle stimulating hormone level and the testicular volume.

Description

technical field [0001] The invention relates to the fields of biological diagnosis and medicine, in particular to a seminal plasma exosome tsRNA marker related to the diagnosis of non-obstructive azoospermia and its application. Background technique [0002] The incidence of azoospermia accounts for 10%-20% of male infertility. According to the presence or absence of sperm in the testis, it can be divided into two categories: one is obstructive azoospermia (OA) with normal spermatogenesis in the testis. The other is non-obstructive azoospermia (NOA), which is a disorder of testicular spermatogenesis. An accurate diagnosis of non-obstructive azoospermia can provide valuable guidance to clinicians. Although conventional biochemical markers in semen, such as citric acid, acid phosphatase, fructose, and α-glucosidase, can be used to assist in the diagnosis of OA patients; testicular volume, serum follicle-stimulating hormone (FSH) levels, etc. can be used to assist in the diag...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 朱作斌李颖韩从辉
Owner 江苏阔然生物医药科技有限公司
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