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A plasma exosomal tsRNA marker associated with the diagnosis of non-obstructive azoospermia and its application

A technology of azoospermia and markers, applied in DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve problems such as inability to effectively diagnose non-obstructive azoospermia

Active Publication Date: 2022-04-15
XUZHOU MEDICAL UNIV
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Problems solved by technology

[0005] One of the purposes of the present invention is to overcome the deficiency that the existing non-invasive diagnostic indicators cannot effectively diagnose non-obstructive azoospermia, and provide a plasma exosomal tsRNA marker related to the diagnosis of non-obstructive azoospermia

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  • A plasma exosomal tsRNA marker associated with the diagnosis of non-obstructive azoospermia and its application
  • A plasma exosomal tsRNA marker associated with the diagnosis of non-obstructive azoospermia and its application
  • A plasma exosomal tsRNA marker associated with the diagnosis of non-obstructive azoospermia and its application

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[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0027] Such as figure 1 As shown: the present invention first analyzes the differential tsRNA in plasma exosomes of non-obstructive azoospermia, obstructive azoospermia, and healthy control population, and then analyzes the selected differential tsRNA in more non-obstructive azoospermia, obstructive azoospermia, Real-time fluorescent quantitative PCR verification was carried out in samples from patients with sexual azoospermia, severe spermatogenic dysfunction and healthy controls, and finally determined whether the screened tsRNA was present in testicular tissue samples from patients with obstructive azoospermia and non-obstructive azoospermia. There are differences in expression to further determine its feasibility as a diagnosis of non-obstructive azoospermia, and to distinguish non-obstructive azoospermia from obstructive azoosper...

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Abstract

The invention discloses a plasma exosome tsRNA marker related to the diagnosis of non-obstructive azoospermia and its application. The marker is tRF-Gly-GCC-002, and the sequence of tRF-Gly-GCC-002 is SEQ ID NO.1. The marker has specificity and sensitivity to non-obstructive azoospermia, and can be used to prepare a diagnostic kit for non-obstructive azoospermia. The plasma exosome marker tRF-Gly-GCC-002 of the present invention is differentially expressed in the plasma of patients with non-obstructive azoospermia and obstructive azoospermia, which can better diagnose non-obstructive azoospermia, and can The effect of predicting the result of sperm retrieval in azoospermia is better than the commonly used indicators such as plasma follicle stimulating hormone level and testicular volume.

Description

technical field [0001] The invention belongs to the field of biological diagnosis and medicine, and specifically relates to a plasma exosome tsRNA marker related to the diagnosis of non-obstructive azoospermia and its application. Background technique [0002] The incidence of azoospermia accounts for 10%-20% of male infertility. According to the presence or absence of sperm in the testis, it can be divided into two categories: one is obstructive azoospermia (OA) with normal spermatogenesis in the testis. The other type is non-obstructive azoospermia (NOA), which is a disorder of testicular spermatogenesis. An accurate diagnosis of non-obstructive azoospermia can provide valuable guidance to clinicians. Although conventional biochemical markers in semen, such as citric acid, acid phosphatase, fructose, and α-glucosidase, can be used to assist in the diagnosis of OA patients; testicular volume, serum follicle-stimulating hormone (FSH) levels, etc. can be used to assist in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/113C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 朱作斌李颖韩从辉
Owner XUZHOU MEDICAL UNIV
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