Plasma exosome miRNA markers related to diagnosis of primary non-obstructive azoospermia and application thereof

A technology of azoospermia and markers, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/testing, etc., can solve the problems of ineffective diagnosis of non-obstructive azoospermia and achieve excellent results

Active Publication Date: 2021-01-08
XUZHOU MEDICAL UNIV
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Problems solved by technology

[0005] One of the purposes of the present invention is to overcome the deficiency that the existing non-invasive diagnostic indicators cannot effectively diagnose non-obstructive azoospermia, and provide a plasma exosomal miRNA marker related to the diagnosis of primary non-obstructive azoospermia

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  • Plasma exosome miRNA markers related to diagnosis of primary non-obstructive azoospermia and application thereof
  • Plasma exosome miRNA markers related to diagnosis of primary non-obstructive azoospermia and application thereof
  • Plasma exosome miRNA markers related to diagnosis of primary non-obstructive azoospermia and application thereof

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Embodiment Construction

[0027] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0028] Such as figure 1 Shown: the present invention first analyzes the differential miRNAs in plasma exosomes of non-obstructive azoospermia, obstructive azoospermia and healthy control population, and then analyzes the selected differential miRNAs in more non-obstructive azoospermia, obstructive azoospermia, Real-time fluorescent quantitative PCR verification was carried out in samples from patients with sexual azoospermia, severe spermatogenic dysfunction and healthy controls, and finally determined whether the screened miRNAs were present in testicular tissue samples from patients with obstructive azoospermia and non-obstructive azoospermia. There are differences in expression to further determine its feasibility as a diagnosis of non-obstructive azoospermia. Each specific plan is implemented as follows:

[0029] 1. Plasma sampl...

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Abstract

The invention discloses plasma exosome miRNA markers related to diagnosis of primary non-obstructive azoospermia and application thereof. The markers are hsa-miR-513c-5p and hsa-miR-202-5p, the sequence of the hsa-miR-513c-5p is shown as SEQ ID NO.1, and the sequence of the hsa-miR-202-5p is shown as SEQ ID NO.2. The markers have specificity and sensitivity to the non-obstructive azoospermia, andcan be used to prepare a diagnostic kit for the non-obstructive azoospermia. The two plasma exosome markers hsa-miR-513c-5p and hsa-miR-202-5p disclosed by the invention are differentially expressed in the plasma of patients with non-obstructive azoospermia and obstructive azoospermia, so that the non-obstructive azoospermia can be diagnosed better, the sperm taking result of azoospermia can be predicted, and the effect is superior to common indexes such as plasma follicle-stimulating hormone level and testis volume.

Description

technical field [0001] The invention belongs to the field of biological diagnosis and medicine, and specifically relates to a plasma exosome miRNA marker related to the diagnosis of primary non-obstructive azoospermia and its application. Background technique [0002] The incidence of azoospermia accounts for 10%-20% of male infertility. According to the presence or absence of sperm in the testis, it can be divided into two categories: one is obstructive azoospermia (OA) with normal spermatogenesis in the testis. The other is non-obstructive azoospermia (NOA), which is a disorder of testicular spermatogenesis. An accurate diagnosis of non-obstructive azoospermia can provide valuable guidance to clinicians. Although conventional biochemical markers in semen, such as citric acid, acid phosphatase, fructose, and α-glucosidase, can be used to assist in the diagnosis of OA patients; testicular volume, serum follicle-stimulating hormone (FSH) levels, etc. can be used to assist i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 朱作斌李颖韩从辉
Owner XUZHOU MEDICAL UNIV
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