A seminal exosomal tsRNA marker associated with the diagnosis of non-obstructive azoospermia and its application
A technology of azoospermia and markers, applied in the direction of DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of ineffective diagnosis of non-obstructive azoospermia
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[0027] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.
[0028] Such as figure 1 As shown: the present invention first analyzes the differential tsRNA in seminal plasma exosomes of non-obstructive azoospermia, obstructive azoospermia and healthy control population, and then analyzes the selected differential tsRNA in more non-obstructive azoospermia, Perform real-time fluorescent quantitative PCR verification in samples from patients with obstructive azoospermia, severe spermatogenic dysfunction and healthy controls, and finally determine whether the screened tsRNA is present in testicular tissue samples from patients with obstructive azoospermia and non-obstructive azoospermia Whether there is a difference in expression, to further determine its feasibility as a diagnosis of non-obstructive azoospermia, and conduct bioinformatics functional analysis to determine its possible regulatory mec...
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