Reagent kit for detecting obstruction performance and non-obstruction performance non-spermatozoa symptom based on Eppin antibody

Abstract

The invention relates to a reagent kit for detecting obstructive and non-obstructive azoospermia based on an Eppin antibody. The reagent kit comprises bovine serum albumin, a phosphate buffer solution, a TBST eluant, a rabbit anti-human Eppin antibody, a goat anti-rabbit IgG antibody, a chromogenic agent, a microfilter and a pyroxylin membrane arranged in the microfilter; in testing, seminal plasma which is to be tested is diluted through the phosphate buffer solution, is adsorbed and fixed on the pyroxylin membrane of the microfilter and is closed through the bovine serum albumin; and the pyroxylin membrane closed by the albumen is subjected to first washing through the TBST eluant, is incubated through the rabbit anti-human Eppin antibody, is subjected to secondary washing through TBST, is incubated through the goat anti-rabbit IgG antibody and is subjected to chromogenic discrimination through the chromogenic agent. The reagent kit has the advantages that the reagent kit has no wound, can alleviate the pain of a patient receiving seminal duct radiography and testicle biopsy, can save mass inspection expense needed by the prior seminal duct radiography and relevant inspection, has high sensitivity, can reach the molecular level, is simple, feasible and rapid and has important application prospect and practical value.

Description

technical field [0001] The invention relates to a kit for detecting obstructive and non-obstructive azoospermia based on Eppin antibody. Background technique [0002] In the cases of male infertility, azoospermia accounts for a large proportion, and the highest incidence rate is reported in the literature as 24.3%. There are many reasons for its pathogenesis, including endocrine factors, genetic factors, physical and chemical factors, drug factors and disease factors. Azoospermia can be caused by a variety of causes, but it can be broadly divided into two categories. One is that the testis has normal spermatogenesis. Due to the obstruction of the vas deferens, the sperm cannot be excreted, which is the so-called obstructive azoospermia (OA); The other type is testicular spermatogenesis dysfunction, and sperm cannot be produced, which is the so-called non-obstructive azoospermia (NOA). There are many etiologies, including genetic factors, endocrine factors, drug factors, phy...

AI Technical Summary

Problems solved by technology

[0005] At present, for patients with azoospermia with normal testicular volume and normal sex hormone levels clinically, in order to distinguish between obstructive azoospermia (OA) and non-obstructive azoospermia (NOA), i.e. primary spermatogenesis disorder, ultimately only rely on complicated procedures. , invasive vasectomy and diagnostic surgical sperm retrieval procedures such as percutaneous epididymis sperm aspiration (PESA) and testicular sperm aspiration (TESA), which are invasive and destructive diagnostic tests, the patient Intimidating, unacceptable, uneconomical and impractical

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