Non-obstructive azoospermia auxiliary diagnosis gene detection kit

Abstract

The invention discloses a non-obstructive azoospermia auxiliary diagnosis gene detection kit, relates to the field of azoospermia diagnosis, and solves the problem of difficult clinical diagnosis of male infertility. The non-obstructive azoospermia auxiliary diagnosis gene detection kit comprises a specific PCR primer, a PCR buffer solution, dNTPs and DNA polymerase, wherein the specific PCR primer is a group of specific primers for detecting the 69826819 mutation site in the non-obstructive azoospermia TEX11 gene. A specific primer SEQ ID NO: 1 and a specific primer SEQ ID NO: 2 are designed,the specific primers have higher specificity, the non-obstructive azoospermia auxiliary diagnosis gene detection kit consisting of the specific primers is adopted to detect the 69826819 mutation sitein the TEX11 gene of a male patient, and the detection rate of the gene mutation is 100%.

Description

technical field

[0001] The invention relates to the technical field of diagnosis of azoospermia, in particular to a gene detection kit for auxiliary diagnosis of non-obstructive azoospermia. Background technique

[0002] Male infertility refers to a couple who have a normal sex life after marriage and do not use any contraceptive measures, and the wife is infertile due to the man's reasons. Male infertility seriously affects men's health. According to the World Health Organization survey, about 8% of couples in the childbearing age suffer from infertility. Based on this, it is estimated that about 50 million to 80 million people in the world suffer from infertility problems , and showed an increasing trend year by year. Among them, the proportion of infertility caused by male reasons is generally not less than 50%. Male infertility can be divided into absolute infertility and relative infertility according to clinical manifestations; it can be divided into primary infertil...

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