New method for analysis of sperm survival rate

A technology for survival rate and sperm motility, applied in the field of analyzing male sperm survival rate

Inactive Publication Date: 2013-12-04
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there are currently no standard experimental guidelines for the analysis of sperm viability using gentian violet staining

Method used

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  • New method for analysis of sperm survival rate
  • New method for analysis of sperm survival rate
  • New method for analysis of sperm survival rate

Examples

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Embodiment 1

[0029] Example 1 Simple Gentian Violet Solution Staining Analysis of Sperm Survival Rate in Semen Samples

[0030] Materials and Methods:

[0031] 1. Obtaining Human Semen Samples

[0032] The semen samples used are provided by volunteers, so several healthy male volunteers are required to provide semen samples according to the following requirements:

[0033] 1) The sample is a sample of normal masturbation and ejaculation, which cannot be obtained from a condom

[0034] 2) The acquisition of samples must be strictly controlled within 3 to 7 days of the last ejaculation

[0035] 3) The semen sample is collected directly into the specially provided sample cup, fill in the ejaculation time and volunteer number, close the sample cup tightly, and send it to the laboratory or designated place within the specified time

[0036] 4) After one ejaculation, all samples must be collected into the sample cup without omission or any non-semen components mixed in

[0037] 5) After the...

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Abstract

The invention relates to a new method for analysis of a male sperm survival rate. A semen sample is stained by a gentian violet solution at a certain concentration, a staining result is observed under a microscope, survival sperms and dead sperms are determined through colored difference, and the sperm survival rate is calculated; after a same sample is stained by an eosin Y standard staining method recommended by the WHO, the sperm survival rate is calculated; a statistical difference between the two sperm survival rates obtained by the two methods is tested by statistics, a result shows that the gentian violet normal saline solution staining method can be used for distinguishing the dead sperms and the survival sperms, almost all of the sperms are colored after being stained by the gentian violet normal saline, sperm motility is decreased, so as to help to count and carry out subsequent analysis under the microscope. The result proves that the method by using the gentian violet solution to stain the semen sample can easily distinguish the survival sperms and the dead sperms, can further analyze the sperm survival rate, can also assist in the analysis of the sperm motility, and contributes to identification and analysis of male sterility.

Description

technical field [0001] The present invention relates to a new method for analyzing the survival rate of male sperm. Use a certain concentration of gentian violet solution to stain the semen sample. The staining results can easily distinguish viable sperm from dead sperm under a microscope, and can further analyze the survival rate of sperm, and can also assist in the analysis of sperm motility, which is helpful for the identification and analysis of male infertility . Background technique [0002] Male infertility is one of the most important problems in human reproduction, and it is the most common clinical problem and serious challenge in andrology. Due to the decline in the quality and quantity of male sperm in the past 30 to 50 years, the incidence of testicular cancer, poor testicular descent, hypospadias and infertility has been increasing, and male infertility has attracted much attention. Although the diagnosis and treatment of male infertility have made significan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N21/29
Inventor 赵永岐刘淑红周明非范明朱望林凯吴燕丁学锋王飞赵彤房涛葛学铭
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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