Rapid typing detection kit for ejaculated sperm cells and rapid typing detection method for ejaculated sperm cells

Abstract

The invention discloses a rapid typing detection kit for ejaculated sperm cells. The kit comprises a PBS solution, a calcium-free HBSS buffer solution, a fixing solution, a staining reagent and a quality control product. The PBS solution and the calcium-free HBSS buffer solution both comprise one or more of bovine serum albumin, fetal bovine serum, glycerol serum, protein and glycerol; the staining reagent comprises a cell permeabilizing agent and a fluorescently labeled antibody. The invention also provides a rapid typing detection method for ejaculated sperm cells by adopting the kit mentioned above. The rapid typing detection kit for ejaculated sperm cells and the rapid typing detection method for ejaculated sperm cells can be used for quickly determining the types and the quantity of spermatogenic cells in the ejaculated sperm cells, further determining whether the ejaculated sperm cells are obstructive or non-obstructive azoospermia, solve the problem that clinical evaluation aiming at the current semen cytology in China lacks a quick and accurate detection reagent in the current clinic, provide guidance for a clinician to select a treatment scheme, and practically solve the problem of a patient.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a test kit for rapid typing of semen cells and a detection method. Background technique [0002] Big data shows that the population of childbearing age in my country is about 230 million, of which about 40 million people suffer from infertility, and about 12 million male patients. The detection of these patients mainly depends on the typing and monitoring of sperm cells. [0003] In addition to sperm, semen also contains exfoliated cells of the seminiferous epithelium and urogenital tract, such as spermatogonia, spermatocytes, sperm cells, Sertoli cells, Leydig cells, epithelial cells, and white blood cells. For patients with azoospermia, it is possible to judge whether it is obstructive or non-obstructive azoospermia by evaluating the type and number of spermatogenic cells in the semen. At present, the evaluation of semen cytology is through routine preparation and staining, an...

AI Technical Summary

Problems solved by technology

[0004] Flow cytometry (Flow Cytometry, FCM) uses flow cytometry as a detection method, and through specific fluorescent labeling of target molecules, it can quickly and accurately perform multi-parameter quantitative analysis and Sorting technology is therefore the most powerful tool to solve the above problems, but so far, no relevant kits have appeared

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