Detection strip for Zika virus detection by means of fast immunochromatography method

A technology for rapid immunization and detection strips, applied in the field of immune detection, which can solve the problems of high price and long detection time.

Inactive Publication Date: 2016-09-21
卢氏实验室公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Zika virus (Zika virus) is transmitted through mosquito bites, and can also be transmitted through the placenta. Currently, there is no specific treatment for Zika virus on the market. For the

Method used

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  • Detection strip for Zika virus detection by means of fast immunochromatography method
  • Detection strip for Zika virus detection by means of fast immunochromatography method
  • Detection strip for Zika virus detection by means of fast immunochromatography method

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The test strip or test box for rapid immunochromatographic detection of Zika virus antibody. The immunodetection strip is divided into 6 areas: the first area is located at one end of the test strip, which is a sample pad, and will be tested during use. The sample is added and dropped on the sample pad in the first area; the second area is next to the first area, which is a small piece of labeled virus antigen solid phase, and the marker on the solid phase small piece of the third area is the labeled antigen as gene protein number 1248; The sixth area is located in the middle of the second area and the third area, which is the nitrocellulose chromatography membrane area, and the nitrocellulose chromatography membrane is fixed on it; the third area is located in the middle of the sixth area and the fourth area, and is the detection area T , coating the antibody spraying zone, which preferably includes a T1 detection line and a T2 detection line, the T1 detection line is f...

Embodiment 2

[0025] A test strip for rapid immunochromatographic detection of Zika virus antigen. The immunodetection strip is divided into 6 areas: the first area is located at one end of the test strip, which is a sample pad, and the sample to be tested is added in drops during use. On the sample pad in the first area; the second area is next to the first area, which is a solid-phase small piece of labeled virus antibody, and the marker on the solid-phase small piece in the third area is a coated antibody: Spray Zika virus monoclonal on the T area Antibody number 084-2 mg / ml; the sixth zone is located in the middle of the second zone and the third zone, which is a nitrocellulose chromatography membrane zone, on which a nitrocellulose chromatography membrane is fixed; the third zone is located in the sixth zone In the middle of the fourth area is the detection area T. The labeled antibody is gold-labeled and another paired monoclonal antibody number 024-2 mg / ml. The fourth area is located ...

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Abstract

The invention provides a detection strip or kit for Zika virus detection by means of the fast immunochromatography method. The immunodetection strip has six areas. The first area is a sample adding pad; the second area is an immobilized Zika antigen marker area or anti-Zika virus antigen specific antibody marker; the third area is a detection area T and a coated antibody spray area, and the area T is matched with the second area; the fourth area is a contrast area used for immobilization of non-specific antibodies; the fifth area is a water absorber; the sixth area is a nitro fiber chromatography film. According to the detection strip or kit, only a small number of samples to be tested are needed, no equipment is required, a detection result can be obtained within ten minutes, detection is easy and fast, and the detection strip or kit can be purchased from a pharmacy so that patients can conduct detection by themselves.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a detection strip or detection box for rapid immunochromatographic detection of Zika virus. Background technique [0002] Since May 2015, the Zika virus epidemic has been rampant in Brazil, South America and Latin America, and has a tendency to spread to the whole world. So far, more than 1.5 million people in Brazil have been infected. The infection has mild symptoms such as fever, rash, joint pain or conjunctivitis, and can cause microcephaly and brain damage in infants, as well as neurological damage and even death in adults. [0003] Zika virus (Zika virus) is transmitted through mosquito bites, and can also be transmitted through the placenta. Currently, there is no specific treatment for Zika virus on the market. For the detection of the virus, PCR is mostly used, but this method requires special The PCR instrument also requires the delicate operation of professional techni...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/577
CPCG01N33/558G01N33/577
Inventor 詹姆斯·儆翊·卢惟钊·卢胡成龙
Owner 卢氏实验室公司
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