Automatic sampling distinguishing chemiluminescent multi-component immunological detection system and analysis method of same

A chemiluminescence and detection system technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, analysis of materials, etc., can solve problems such as heavy labor, achieve less manual operation, simple operation, and reduced analysis. cost effect

Inactive Publication Date: 2009-09-30
JIANGSU CANCER HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the operations are done manually, the workload is heavy, and the time required is mostly more than 2 hours, which is not suitable for high-throughput rapid automated analysis

Method used

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  • Automatic sampling distinguishing chemiluminescent multi-component immunological detection system and analysis method of same
  • Automatic sampling distinguishing chemiluminescent multi-component immunological detection system and analysis method of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: in combination with figure 1 Further description of the four-component detection system:

[0033] The detection system is composed of an incubation system, a multi-channel sample injection system, a signal resolution acquisition system and a computer. For the four-component detection, the incubation system consists of four glass test tubes with a diameter of 0.8 cm, a height of 2 cm, and an inner diameter of 0.5 cm equipped with a micro-stirrer and a magnetic stirrer in a constant temperature water bath. The glass test tubes are placed in the magnetic stirrer. In a constant temperature water bath; the multi-channel sampling system is composed of a sampling channel, a magnet and a glass tube. The sampling channel is a polytetrafluoroethylene tube with an inner diameter of 0.8 mm. The magnet is placed under the glass tube to collect samples after incubation. magnetic beads; the solution delivery system consists of a two-way multi-channel peristaltic pump, a...

Embodiment 2

[0034] Example 2: Chemiluminescent four-component immunoassay method:

[0035] The specific analysis process is shown in Table 1.

[0036]

[0037] All analysis steps are programmed and automatically controlled by computer, and the measured chemiluminescent signal is output from the computer. After the analysis steps were completed, the magnetic beads immobilized with antibodies were collected uniformly, soaked in glycine / hydrochloric acid buffer solution with pH 2.2 for 10 minutes, and washed with 0.01M phosphate buffer solution (pH 7.4) for reuse.

Embodiment 3

[0038] Example 3: Taking four markers with significant significance in the clinical diagnosis of tumors: alpha-fetoprotein (AFP), cancer antigen 125 (CA125), cancer antigen CA199 (CA199), and carcinoembryonic antigen (CEA) as examples, The application of the automatic sample injection resolution chemiluminescence multicomponent immunoassay method is illustrated.

[0039] Use epoxy-activated magnetic beads as the carrier of immune reaction, covalently immobilize mouse monoclonal AFP antibody, mouse monoclonal CA125 antibody, mouse monoclonal CA199 antibody, mouse monoclonal CEA antibody, and block residual activity with bovine serum albumin site. The tracer antibodies are sheep polyclonal AFP, CA125, CA199, and CEA antibodies labeled with alkaline phosphatase.

[0040] As shown in Table 1, four tumor markers in clinical serum samples were used as detection objects, and four-component chemiluminescent immunoassay was performed to obtain the concentrations of AFP, CA125, CA199, ...

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Abstract

The invention relates to an automatic sampling distinguishing chemiluminescent multi-component immunological detection system and an analysis method of the same. An incubation system of the detection system consists of a test tube (1), a stir bar (2), magnetic beads (3) and a constant temperature magnetic stirring apparatus (11); a multi-channel sampling system consists of sampling channels (4), a magnet (12) and a glass tube (13); a solution conveying system consists of a peristaltic pump (14), a connecting pipe (10), a multifunctional syringe valve (15) and a multiposition valve (16); a signal acquisition system consists of a detection channel (9), a plane mirror (17) and a multiplier phototube (18); and the method is to fix various capture antibodies on the functionalized surfaces of the magnetic beads to perform specific reactions with an object to be detected and a tracing antibody so as to form a sandwiched complex, introduce chemiluminescent substrate liquid into the sandwiched complex after collecting and washing off excessive enzyme labeled antibodies by using the magnet, and inject the chemiluminescent substrate liquid into detection channels sequentially to perform detection. The method has the characteristics of simplicity, quickness, good reproducibility, high flexibility, low cost, and the like, and can be applied to the fields of clinical diagnosis, environmental monitoring, food security, and the like.

Description

1. Technical field [0001] The invention is a sample-resolving multi-component chemiluminescence immunoassay technique, and relates to an immunoassay method for nearly simultaneous detection of multiple analytes in the same sample. The magnetic beads are used as immune reaction carrier, and combined with chemiluminescence and flow analysis technology; the invention also relates to an automatic detection instrument system for multi-component chemiluminescence immunoassay. 2. Background technology [0002] Immunoassay has the advantages of high selectivity, high sensitivity, and good universality, and has a wide range of applications in the fields of food safety, environmental monitoring, clinical diagnosis, drug analysis, and microbial testing. When immunology is applied to practical fields, it is often necessary to determine the content of multiple components in complex samples. For example, in cancer diagnosis, the simultaneous determination of multiple tumor markers provide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/546G01N21/76
Inventor 严枫刘宏鞠熀先
Owner JIANGSU CANCER HOSPITAL
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