Patents
Literature
Patsnap Copilot is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Patsnap Copilot

71results about How to "Save costly" patented technology

Automatic sampling distinguishing chemiluminescent multi-component immunological detection system and analysis method of same

The invention relates to an automatic sampling distinguishing chemiluminescent multi-component immunological detection system and an analysis method of the same. An incubation system of the detection system consists of a test tube (1), a stir bar (2), magnetic beads (3) and a constant temperature magnetic stirring apparatus (11); a multi-channel sampling system consists of sampling channels (4), a magnet (12) and a glass tube (13); a solution conveying system consists of a peristaltic pump (14), a connecting pipe (10), a multifunctional syringe valve (15) and a multiposition valve (16); a signal acquisition system consists of a detection channel (9), a plane mirror (17) and a multiplier phototube (18); and the method is to fix various capture antibodies on the functionalized surfaces of the magnetic beads to perform specific reactions with an object to be detected and a tracing antibody so as to form a sandwiched complex, introduce chemiluminescent substrate liquid into the sandwiched complex after collecting and washing off excessive enzyme labeled antibodies by using the magnet, and inject the chemiluminescent substrate liquid into detection channels sequentially to perform detection. The method has the characteristics of simplicity, quickness, good reproducibility, high flexibility, low cost, and the like, and can be applied to the fields of clinical diagnosis, environmental monitoring, food security, and the like.
Owner:JIANGSU CANCER HOSPITAL +1

Large-size impeller processing technique in precise casting

InactiveCN102699279AReduce finishing workloadLow densityFoundry mouldsFoundry coresThin shellsImpeller
The invention provides a large-size impeller processing technique in precise casting and belongs to the technical field of special metal article processing. The large-size impeller processing technique comprises the following steps of: firstly, utilizing plastic foam as a model, integrally adhering a pouring system foam mould with a casting foam mould to form a module, and then, coating a plurality layers of refractory materials on the outer surface of the module, and sintering to remove the pouring system foam mould and the casting foam mould to form a thin ceramic shell with a die cavity; putting the prepared thin ceramic shell in a sand box, guaranteeing the minimal distance from the outer wall of the thin shell to the wall of the inner cavity of the sand box to be 40-50mm, and making a pouring gate cup on the thin shell be 40-70mm higher than the sand box, filling resin sand between the sand box and the thin shell, and vibrating the sand box while filling the resin sand until the sand box is fully filled; heating the sand box to 350-500 DEG C; and finally pouring molten metal through a stopper ladle. The large-size impeller processing technique provided by the invention can both guarantee the casting dimension precision and good smoothness, but also guarantee no cold shut or crack on the casting, thereby greatly reducing the finishing workload of the casting, reducing the production cost, shortening the working period and improving the casting quality stability.
Owner:安徽应流铸业有限公司

Optical network fault detection device based on noise signals and detection method thereof

Provided are an optical network fault detection device based on noise signals and a detection method thereof. The device is characterized in that a noise source and a signal processing unit are arranged in a main optical fiber between an optical line terminal and an optical distribution network unit. The noise source includes spontaneous radiation noise amplified by an online optical amplifier or introduced noise signals and is located on the side of the optical line terminal. The method includes utilizing spontaneous radiation noise amplified by the optical amplifier in an optical fiber main line or introduced noise signals, conducting detection by coupling a wavelength division multiplexer into branches of the optical network, filtering optical signals of corresponding branches to be detected through a reflection type adjustable filter, conducting relative processing on returned detection signals and reference signals in the signal processing unit and achieving fault location through relative peak value of relative curves. The device and the method utilize noise wideband characteristics to achieve proper online location detection of the wavelength division multiplexing passive optical network, and are simple in structure, convenient to operate and accurate in optical network fault detection.
Owner:TAIYUAN UNIV OF TECH

LAMP (Loop-Mediated Isothermal Amplification) primer group and method for rapidly detecting ditylenchus destructor from complex samples

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer group and method for rapidly detecting ditylenchus destructor from complex samples. The LAMP primer group comprises a pair of outer primers F3/B3, a pair of inner primers FIP/BIP and a loop primer LF, and sequences of the primers are sequentially shown as SEQ ID NO. 1-5. The LAMP primer group and the detection method thereof disclosed by the invention have excellent specificity and sensitivity for detecting the ditylenchus destructor, individuals of the ditylenchus destructor in different insect stages (such as eggs, larva, female and male) can be detected, the ditylenchus destructor can be directly detected from multiple nematode mixed samples, plant tissue samples and soil samples, and the detection sensitivity can reach 1/1000 individual DNA. A novel detection method is provided for rapid detection and identification and early diagnosis of the ditylenchus destructor, and the method has the advantages ofbeing accurate, sensitive, stable and intuitive in result judgment. The LAMP primer group disclosed by the invention is simple and convenient to operate and high in practicality, and has important significances and application value on quarantine on ports and transfer and origin monitoring operations.
Owner:SOUTH CHINA AGRI UNIV

Molecular detection method for rapidly identifying carbendazim-resistant genotype-F200Y botrytis cinerea strain

ActiveCN104313177ARapid identificationRich technology system for resistance detectionMicrobiological testing/measurementMicroorganism based processesResistant genotypeSky
The invention discloses a molecular detection method for rapidly identifying a carbendazim-resistant genotype-F200Y botrytis cinerea strain. The molecular detection method is a molecular detection technology, which is established based on a LAMP (loop-mediated isothermal amplification) technology and is rapid, convenient, high in specificity and sensitivity and low in cost. The detection method comprises the following steps of designing two pairs of specific primers on a beta tubulin of a detected sample, performing LAMP, and judging whether the detected sample is a carbendazim-resistant genotype-F200Y botrytis cinerea strain or not according to the color of a reaction product; if the LAMP product is sky-blue and an electrophoretogram is a ladder band, determining that the product amplification exists and that the detected sample is the carbendazim-resistant genotype-F200Y botrytis cinerea strain; if the LAMP product is purple and the electrophoretogram is free of band, determining that the product amplification does not exist and that the detected sample is a carbendazim-nonresistant genotype-F200Y botrytis cinerea strain. The method is convenient, rapid, low in cost and significant for theoretically guiding the resistance risk evaluation and reasonable medication of botrytis of crops.
Owner:NANJING AGRICULTURAL UNIVERSITY

Fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim based on LAMP (loop-mediated isothermal amplification) technology

The invention discloses a fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim. The method can be used for performing dynamic monitoring and early warning on botrytis cinerea bacteria causing gray mold of crops for the resistance groups of the carbendazol. In the method disclosed by the invention, a molecular detection technology having the advantages of fastness, convenience, strong specificity, high sensitivity and low cost is established on the basis of a loop-mediated isothermal amplification technology (LAMP). The detection method comprises the following steps: designing 2 pairs of specific primers on beta tubulin of high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol, performing LAMP amplification, and judging whether products are the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol or not according to the colors of reaction products. If the LAMP amplification products appear to be azure, electrophoresis patterns present ladder-shaped strips, and the product amplification is generated, the products are identified as the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol; if the LAMP amplification products present violet, the electrophoresis patterns do not have strips, and the product amplification is not generated, then the products are identified as non high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol. The method is simple, convenient, fast, and low in cost and has important actual significance in the resistance risk assessment of the gray mold of the crops and reasonable medication.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for preparing local enhancement aluminum matrix composite

InactiveCN103831421AImproves local wear resistance and overall toughnessEnergy savingAluminum matrix compositesMetal matrix composite
The invention relates to a method for preparing a local enhancement aluminum matrix composite and belongs to the technical field of preparing metal matrix composites. Al powder, Ti powder, graphite powder and a pure Al block are used as raw materials. The method includes the following steps that firstly, the Al powder, the Ti powder and the graphite powder are prepared to form Al-Ti-C mixed powder, and the Al-Ti-C mixed powder receives ball milling and is evenly mixed; secondly, the Al-Ti-C mixed powder is pressed to form a preformed blank which is placed into a graphite mold to be preheated to 500 DEG C; thirdly, the pure Al block is smelted to 940 DEG C through a crucible resistance furnace and then fast poured into the graphite mold, the preformed blank is ignited, a synthetic reaction happens, and the local enhancement aluminum matrix composite is obtained. The method for preparing the local enhancement aluminum matrix composite has the advantages of being fast in reaction, simple in technology, capable of saving energy, efficient, good in filling capacity, high in connection degree of a matrix and enhanced particles and the like. In addition, enhancement phase TiC particles are prevented from being contaminated by impurities such as oxygen, the method can be applied to the field of composites which only need local enhancement, and an expensive enhancement phase is omitted.
Owner:TONGREN UNIV

LAMP primer group for fast detecting and identifying aphelenchoides ritzemabosi and detection method thereof

The invention discloses an LAMP primer group for fast detecting and identifying aphelenchoides ritzemabosi and a detection method thereof. The primer group comprises a pair of outer primers F3/B3, a pair of inner primers FIP/BIP and a loop primer LF; and the sequences of the primers are respectively and sequentially shown as SEQ ID NO.1 to 5. An LAMP detection method of the aphelenchoides ritzemabosi is built by using the LAMP primer group. An amplification product can be detected by an electrophoresis method or fluorochrome method; when stepped stripes occur in electrophoresis detection or when green fluorescence can be observed after the fluorescence dye addition, the result proves that the aphelenchoides ritzemabosi is contained in a sample to be inspected. The detection method can be used for detecting and identifying individuals of aphelenchoides ritzemabosi in different insect states (female insects, male insects, larvae or ova); the aphelenchoides ritzemabosi can be directly detected from various-nematoid mixed samples and plant tissue samples; the detection sensitivity reaches 1/100 single insect DNA; and the LAMP primer group and the detection method are particularly applicable to port and transfer quarantine inspection and production place monitoring.
Owner:SOUTH CHINA AGRI UNIV

LAMP (loop-mediated isothermal amplification)-based method for rapid detection of sclerotinia sclerotiorum strain with high resistance to carbendazim

The invention discloses an LAMP (loop-mediated isothermal amplification)-based method for rapid detection of a sclerotinia sclerotiorum strain with high resistance to carbendazim. The method can be used for dynamic monitoring and epidemic warning of field sclerotinia sclerotiorum for a carbendazim resistance group, and is a molecular technique established based on LAMP and used for rapid detection of the sclerotinia sclerotiorum strain with high resistance to the carbendazim. According to the detection method, two pairs of LAMP specific primers are designed on beta-tubulin (comprising 198 amino acid mutation sites) of the sclerotinia sclerotiorum strain with high resistance to the carbendazim, and LAMP is performed; whether the strain is the sclerotinia sclerotiorum strain with high resistance to the carbendazim is determined according to the color of a reaction product; if the color is sky blue (an amplification product exists), the strain is the sclerotinia sclerotiorum strain with high resistance to the carbendazim; and if the color is purple (the amplification product does not exist), the strain is the sclerotinia sclerotiorum strain sensitive to the carbendazim. The method is simple, convenient, fast and low in cost, and has important practical significance for sclerotinia sclerotiorum resistance monitoring and rational drug use.
Owner:NANJING AGRICULTURAL UNIVERSITY

Chemiluminescent immunological detection system with microbubble-accelerated immunoreaction

The invention relates to a chemiluminescent immunological detection system with microbubble-accelerated immunoreaction, which comprises a solution conveying system, a micro-bubble incubation device, an immunosensor, a chemiluminescent detector and a computer. The conveying system consists of peristaltic pumps (P1, P2), a micro syringe pump (P3), a connecting pipe and an eight-way syringe valve; and the immunosensor is made of colorless transparent glass pipes filled with antibody-modified carboxyl-activated resin microballoons and is placed above an optical window of the chemiluminescent detector. A sample to be detected and an enzyme labeled antibody are added into an incubation pipe, nitrogen is introduced to the incubation pipe to generate micro bubbles, accelerate the immunoreaction and reduce the preincubation time, and the micro syringe pump is used for injecting an incubation complex into the immunosensor to form a sandwiched immune complex. Washing liquor is introduced in by the peristaltic pump (P1) to rinse uncombined excessive antibodies, small amount of chemiluminescent substrate of horseradish peroxidase is injected into the mixture by the peristaltic pump (P2), then carrying current is passed in by the peristaltic pump (P1) to carry a substrate zonal belt into the immunosensor and generate chemiluminescent signals, and the concentration of the sample is detected by the signal intensity. The reproduced liquid is introduced into the mixture by the peristaltic pump (P1) after the detection and a reproducible immunosensor performs the next analysis flow. The detection system has the advantages of low cost, quickness, high sensitivity, reproducibility and good precision, and is suitable for the fields of environmental monitoring, clinical diagnosis, food security, and the like.
Owner:JIANGSU CANCER HOSPITAL +1

Photovoltaic building panel

The invention provides an integrative photovoltaic building solar panel. The solar panel comprises three parts, including a photovoltaic layer, lithium battery contravariant units and a foaming pinchplate layer, wherein the photovoltaic layer is a photovoltaic battery array of a solar silicon panel layer; each lithium battery contravariant unit comprises a lithium storage battery, a controller, an inverter, an AC distributor, a monitoring system, a DC load and an AC load which are connected by a circuit and manufactured into a cylindrical structure, and the cylindrical structure is filled ina preset hollow part of the foaming layer; the aggregate of the foaming pinch plate layer is a metal pinch plate structure of which the middle is foamed by perlite, and a plurality of cylindrical hollow parts for placing the lithium battery contravariant units are prefabricated inside the metal pinch plate structure. The photovoltaic solar panel for a building can furthest concentrate spaces, be used for generating electricity on site and reduce expenses and energy consumption in the transportation process of current, thereby avoiding additionally occupied precious building spaces for placinga photovoltaic array panel and realizing the integration of building structures, thus the photovoltaic solar panel can be applied to apricus walls or ceilings of buildings.
Owner:苏州同济材料科技股份有限公司

Lithium battery management system

The invention discloses a lithium battery management system, which comprises N voltage acquisition circuits used for acquiring charging and discharging information of a lithium battery cell, wherein the voltage acquisition circuit comprises an analog front-end module and a displacement cache module; each analog front-end module of the N voltage acquisition circuits is cascaded with a micro-controlmodule through the corresponding displacement cache module, the time sequence switching of the voltage acquisition circuits is achieved to perform monitoring and management on each battery cell of the lithium battery, and except for the first-stage voltage acquisition circuit, a level switching circuit is connected between the displacement cache module and the micro-control module of the other voltage acquisition circuits. By using the analog front-end module, the cost is lower than that of the same digital front end on the market, an expensive isolation chip is saved, and the cost is furtherreduced; and a low-voltage control signal is converted into a control signal of a high-end cascade chip through the level switching module, the isolation problem of a high-voltage circuit and a weak-current circuit does not need to be considered, and the high-voltage problem and the failure problem of an isolation chip are avoided.
Owner:广东锂纬科技有限公司

A kind of hit solar cell and its electrode preparation and connection method

ActiveCN104037265BConductivity advantageReduce surface occlusionFinal product manufacturePhotovoltaic energy generationHeterojunctionConduction band
The invention discloses a HIT (Heterojunction with Intrinsic Thin Layer) solar cell and electrode preparation and series connection methods thereof. The HIT solar cell comprises a metal wire conduction band and solar cell pieces, wherein each solar cell piece comprises an N type silicon plate; the front side of each N type silicon plate is provided with an intrinsic amorphous silicon film and a P type amorphous silicon film; the back side of each N type silicon plate is provided with an intrinsic amorphous silicon film and an N type amorphous silicon film in sequence; the P type amorphous silicon films and the N type amorphous silicon films are provided with transparent conduction oxide films; one solar cell piece is arranged below the front half portion of the metal wire conduction band; the back half portion of the metal wire conduction band is provided with one solar cell piece. Compared with the conventional HIT solar cell, the method has the advantages that a main gate electrode and a fine gate electrode do not need to be printed, and solar cells can be connected in series without bus bars. On one hand, the metal wire portions of the metal wire conduction band are arranged in parallel on the front side or back side of the solar cell, and current on the surfaces of solar cells is collected and conducted out to realize the functions of a fine gate and a main gate; on the other hand, two solar cells are connected in series to serve as a bus bar.
Owner:陕西众森电能科技有限公司

Method for detecting gene F200Y of fusarium graminearum resisting carbendazim on basis of loop-mediated isothermal amplification technology and primer composition

The invention discloses a method for detecting gene F200Y of fusarium graminearum resisting carbendazim on basis of a loop-mediated isothermal amplification technology and a primer composition. The primer composition for detecting carbendazim-resisting gene-type F200Y fusarium graminearum consists of a forward inner primer FIP shown as SEQ ID NO.2, an inverse inner primer BIP shown as SEQ ID NO.3, a forward outer primer F3 shown as SEQ ID NO.4 and a forward outer primer B3 shown as SEQ ID NO.5. The detection method is simple, easy, good in practicability, high in sensitivity, high in specificity, high in accuracy and capable of realizing isothermal amplification, a novel technical platform is provided for the detection of the carbendazim-resisting gene-type F200Y fusarium graminearum, the carbendazim-resisting colony of fusarium graminearum can be monitored, the development state of the resistance colony can be known in time, and the method and the primer composition have important significance for governing the drug resistance of wheat scab and scientifically instructing the drug application, reducing the production cost and reducing the environmental pollution of chemicals.
Owner:NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products