The invention relates to a chemiluminescent immunological detection
system with microbubble-accelerated immunoreaction, which comprises a solution conveying
system, a micro-bubble incubation device, an immunosensor, a chemiluminescent
detector and a computer. The conveying
system consists of peristaltic pumps (P1, P2), a micro
syringe pump (P3), a connecting
pipe and an eight-way
syringe valve; and the immunosensor is made of colorless transparent glass pipes filled with
antibody-modified carboxyl-activated resin microballoons and is placed above an optical window of the chemiluminescent
detector. A sample to be detected and an
enzyme labeled
antibody are added into an incubation
pipe,
nitrogen is introduced to the incubation
pipe to generate micro bubbles, accelerate the immunoreaction and reduce the preincubation time, and the micro
syringe pump is used for injecting an incubation complex into the immunosensor to form a sandwiched
immune complex. Washing liquor is introduced in by the
peristaltic pump (P1) to rinse uncombined excessive antibodies, small amount of chemiluminescent substrate of
horseradish peroxidase is injected into the mixture by the
peristaltic pump (P2), then carrying current is passed in by the
peristaltic pump (P1) to carry a substrate zonal belt into the immunosensor and generate chemiluminescent signals, and the concentration of the sample is detected by the
signal intensity. The reproduced liquid is introduced into the mixture by the peristaltic pump (P1) after the detection and a reproducible immunosensor performs the next analysis flow. The detection system has the advantages of low cost, quickness, high sensitivity, reproducibility and good precision, and is suitable for the fields of
environmental monitoring,
clinical diagnosis, food security, and the like.