LAMP primer group for fast detecting and identifying aphelenchoides ritzemabosi and detection method thereof

A detection method, the technology of chrysanthemum leaves, is applied in the direction of biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., which can solve the problems of narrow detection range, complicated technology, high detection cost, etc., and achieve high accuracy and improved High amplification speed and high sensitivity
CN106520995AActive Publication Date: 2017-03-22SOUTH CHINA AGRI UNIV
3 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2017-03-22

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an LAMP primer group for fast detecting and identifying aphelenchoides ritzemabosi and a detection method thereof. The primer group comprises a pair of outer primers F3 / B3, a pair of inner primers FIP / BIP and a loop primer LF; and the sequences of the primers are respectively and sequentially shown as SEQ ID NO.1 to 5. An LAMP detection method of the aphelenchoides ritzemabosi is built by using the LAMP primer group. An amplification product can be detected by an electrophoresis method or fluorochrome method; when stepped stripes occur in electrophoresis detection or when green fluorescence can be observed after the fluorescence dye addition, the result proves that the aphelenchoides ritzemabosi is contained in a sample to be inspected. The detection method can be used for detecting and identifying individuals of aphelenchoides ritzemabosi in different insect states (female insects, male insects, larvae or ova); the aphelenchoides ritzemabosi can be directly detected from various-nematoid mixed samples and plant tissue samples; the detection sensitivity reaches 1 / 100 single insect DNA; and the LAMP primer group and the detection method are particularly applicable to port and transfer quarantine inspection and production place monitoring.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of molecular detection of plant diseases, and in particular relates to a LAMP primer set for rapid detection and identification of Nematode chrysanthemum leaf nematode and a detection method thereof. Background technique

[0002] Aphelenchoides ritzemabosi (Schwartz, 1911) Steiner&Buhrer, 1932, also known as chrysanthemum ritzemabosi or axillary bud nematode, is a plant pathogenic nematode that parasitizes aboveground parts of plants and causes damage. Together, A. besseyi and A. fragariae are called foliar nematodes. Chrysanthemum nematode has a wide range of hosts, and can parasitize more than 200 kinds of plants, including ornamental plants, vegetables, small fruit plants and other wild plants. The most typical host is chrysanthemum (Dendranthema morifolium). Chrysanthemum leaf blight nematode harms host plants, which will reduce or lose its economic value, and seriously cause plant death. Chrysanthemum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More