The invention discloses a
pretreatment method of a gusset-fish tooth slice for micro-chemical analysis. The
pretreatment method comprises the following steps: collecting
mandibular teeth of a dead gusset-fish individual; cleaning the teeth, removing soft tissues on the surfaces and in tooth roots, and airing the
cleaned teeth; the teeth are fixed on a base made of
epoxy resin blocks, and the base is fixed in a mold; preparing an
epoxy resin
reagent, and embedding the teeth in
epoxy resin; a low-speed precise
cutting machine is used for
cutting the buccal side and the lingual side of the tooth along the
coronal plane, so that the central axis of the
coronal plane of the tooth is contained in a slice with the thickness of 3 mm; further
grinding and
polishing one side of the slice, and fixing the slice on a
glass slide; and
grinding and
polishing the other side of the slice, cleaning, and
drying. The dolphin tooth slice obtained through the
pretreatment method has the advantages of being smooth in surface, precise in
polishing, not prone to breaking and
cracking, capable of achieving growth axial surface maximization and the like, and can be used for microchemical analysis and growth
layer group analysis of dolphin teeth.