Method for determining anti-nucleosome antibody IgG (intravenous gamma globulin) and reagent device

Abstract

The invention provides a method for realizing immunization detection for an anti-nucleosome antibody IgG (intravenous gamma globulin) based on the enzyme-linked immunoassay principle and a reagent device therefor; and an analytic method, the reagent device and an assorted reagent are independently, individually and disposably used for detecting the anti-nucleosome antibody IgG based on enzyme-linked immunoassay. Various reagents needed by enzyme-linked immunoassay for the anti-nucleosome antibody IgG are contained in one analytic device; and by using the method, the related immunology detection can be conveniently carried out according to the using requirements of detection items so as to provide better basis for clinical application.

Description

technical field [0001] The application of the present invention relates to a method and a reagent device for measuring anti-nucleosome antibody IgG, which belong to the technical field of biological detection. Background technique [0002] Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease that affects the connective tissue of the whole body and damages multiple organs. The disease is a connective tissue disease involving multiple systems, characterized by chronic non-suppurative inflammation, with complex and diverse clinical manifestations. , endocrine factors, drug factors, environmental factors and so on. [0003] Nucleosomes are the main autoantigens in SLE, and the nucleosomes are highly immunogenic, driving helper T cells for autoimmune responses and inducing the production of anti-nucleosome antibodies (AnuA). AnuA only acts on natural nucleosomes and nucleosome substructure (H2A-H2B) DNA, but does not react with DNA or histones, and th...

AI Technical Summary

Problems solved by technology

[0011] (1) Only qualitative and semi-quantitative analysis can be carried out, and the specific amount of the tested substance cannot be obtained

[0012] (2) The operation steps are cumbersome and the test takes a long time

[0013] (3) The detection sensitivity needs to be improved

[0016] (1) Non-specific recognition cannot be distinguished according to the size of the molecular weight when analyzing the results

[0017] (2) The operation is relatively complicated and requires a more expensive fluorescence microscope, which is difficult to promote in many primary hospitals and is not suitable for laboratories with a large number of specimens

[0018] (3) The background in the fluorescence measurement is relatively high, and it is difficult to use the fluorescence immunoassay technique for quantitative determination

[0019] (4) Experienced professionals are required to judge the results, and the objectivity of the analysis results is insufficient

[0023] (2) There can be as many as 11 kinds of reagents used for quantitative determination, and each detection reagent should be contained in a reagent bottle, and each time a reagent is used, it is necessary to replace the suction nozzle to add to the microwell plate respectively. In the well, not only are there many types of reagent bottles, but also the operation of adding reagents is extremely cumbersome. If you do not use a fully automatic enzyme immunoassay analyzer, all operations must be done manually, and the price of a fully automatic enzyme immunoassay analyzer is very expensive. , a large investment;

[0024] (3) There is no barcode of reagent information for each test or each test. The production batch number and expiry date information of the test reagent can only be known or known by checking the label on the outer packaging box of the test kit, and the known information is not available during the test process. Controlled, with great discretion;

[0025] (4) The detection reagent is an open method during the detection process, which is likely to cause cross-contamination between various reagents and affect the detection results;

[0026] (5) When the automatic enzyme immunoassay analyzer is not used for detection, the detection process is manual operation, the addition of reagents or samples is not very precise, the operation process is extremely cumbersome and complicated, and operation errors are prone to occur, resulting in inaccurate detection results and high imprecision;

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