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Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune

A technology of immune labeling and quantum dots, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve problems such as unfavorable observation, achieve the effect of maintaining immune activity, improving sensitivity, and increasing the number of quantum dots

Inactive Publication Date: 2009-09-09
SOUTHEAST UNIV
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Problems solved by technology

However, the element content and fluorescence intensity of a single quantum dot are restricted by its own particle size and dispersion state, which is not conducive to observation.

Method used

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  • Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune
  • Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune
  • Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune

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Embodiment Construction

[0023] For specific implementation examples, take the related experiment of α-fetoprotein as an example:

[0024] 1) Preparation of cadmium antimonide quantum dot immunolabel:

[0025] (1) Silanization of the surface of silica microspheres: Add 0.022g of nano-silica microspheres with a particle size of 200±3nm into 2mL of ethanol, and after ultrasonic dispersion for 30 minutes, slowly add 0.4mL of 3-aminopropyltriethyl oxysilane, stirred for 6 hours, centrifuged at 10,000 rpm for 30 minutes, and washed the precipitate with ethanol solution 4 times to obtain amino-modified silica microspheres.

[0026] (2) Preparation of quantum dot-coated silica microspheres: Add the above-mentioned amino-modified silica microspheres to 2 mL of 5 mg / mL commercially available mercaptopropionic acid-modified CdTe quantum dots (excess) and 1 mL of 20 mg / mL 1-( 3-Dimethylaminopropyl)-3-ethylcarbodiimide salt mixed solution, stirred at 4°C for 12 hours. After centrifugation at 10,000 rpm for 30 m...

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Abstract

The invention relates to preparation for a cadmium antimonide quantum dot immune marker and a detection method for electrochemical sandwich immune, having the signal amplification function. Through the reaction of silicon dioxide particle and reacting ATPS, an active amido group is modified on the surface of the silicon dioxide particle under room temperature. The silicon dioxide particle is then reacted with carboxyl on the surface of a quantum dot to form a silicon dioxide microsphere with the surface modified with the quantum dot. The silicon dioxide microsphere after being modified is further modified with a second antibody on the surface of the quantum dot under the actions of EDC and NHS, and a cadmium antimonide quantum dot immune marker is obtained. The detection method for the electrochemical sandwich immune utilizes anti-AFP to modify Fe3O4 magnetic nano-microsphere, thereby Si / QD / Ab2 is modified on magnetic particles, and then electrochemical determination is carried out. Because the surface of the marker is rich in the quantum dots, the amount of the quantum dot loaded in a single sandwich immune process is greatly increased, dissolving-out voltammetry detection signals of an anode are amplified, and the sensitivity of low-concentration biomolecule detection is greatly enhanced.

Description

technical field [0001] The invention relates to a preparation technology of a cadmium antimonide quantum dot CdTe immunolabel, especially a silica sphere SiO co-modified by CdTe and the second antibody Ab2 with signal amplification function 2 Preparation method of immune marker and its application in biomolecular detection. Background technique [0002] During the treatment of tumor patients, sensitive and accurate detection of tumor-associated protein factors in serum is of great significance for tumor treatment and prognosis. Alpha-fetoprotein (AFP) is a glycoprotein with a molecular weight of about 70 kDa, which is usually excreted by the liver, yolk sac and digestive tract during the growth of fetuses and newborns. AFP in healthy adults, its value is lower than 25ng / mL. Increased levels of AFP in serum are seen as an early sign of some cancers, including liver cancer, yolk sac tumor, gastric cancer metastasized from liver tissue, testicular cancer, and nasopharyngeal c...

Claims

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Application Information

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IPC IPC(8): G01N33/532G01N33/551G01N27/26
Inventor 刘松琴陈丽媛陈成良
Owner SOUTHEAST UNIV
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