15 results about "Surface plasmon resonance sensor" patented technology

The invention discloses an immune quantitative detection device for tumor markers, relates to the technical field of medical detection, and aims to solve the problems of complicated operation, long detection period and poor portability in immune quantitative detection of tumor markers in the prior art. Comprising a shell, a disposable detection card, a multi-channel micro-fluidic chip, a high-sensitivity sensing system, a micro magnetic control incubation module, an AI auxiliary image recognition system and a wireless transmission module, the disposable detection card is used for accommodating the multi-channel micro-fluidic chip and a reaction reagent, and the multi-channel micro-fluidic chip is respectively aligned with the high-sensitivity sensing system and the micro magnetic control incubation module; the high-sensitivity sensing system is composed of a surface plasma resonance sensor and a quantum dot fluorescence labeling module, and the surface of a sensing chip of the surface plasma resonance sensor is modified with a tumor marker specific capture antibody. The method has the advantages of convenient detection and high efficiency and precision.

The invention relates to the field of optical fiber sensing, in particular to a multichannel optical fiber surface plasma resonance sensing probe as well as a preparation method and application thereof. According to the multichannel optical fiber surface plasma resonance sensing probe, the sensing probe adopts an end face reflection type optical fiber sensing structure, and the end face reflection type structure is formed by welding a multimode optical fiber and a coreless optical fiber. A multimode-coreless optical fiber welded end face reflection type structure is adopted, SPR signals are excited through end face total reflection, the defect that a traditional lateral coupling type optical fiber SPR sensor is difficult to carry out directional detection in narrow spaces (such as blood vessels and tissue gaps) is overcome, miniaturized insertion type diagnosis with the minimum diameter being 300 micrometers is achieved, and the SPR sensor is suitable for in-situ monitoring of living tissue; according to the invention, through the nanocluster modification layers (0 / 2 / 4 layers) which are axially distributed at intervals, multi-channel functionalization can be completed only by a pulling method, traditional micro-nano processing and a complex film layer structure are not needed, and the preparation time of the multi-channel sensor is shortened from gt to gt; and the time is shortened from 6 hours to 1 hour.

A surface plasmon resonance sensor system includes a sensor plate having a quartz glass substrate and a copper film sputtered onto the substrate and annealed for about two hours. A quartz prism is attached to a surface of the sensor plate, and a microfluidics unit containing carrier fluid is attached to the opposite surface. A target analyte reservoir is connected through a pump to inject target analytes into the microfluidics unit. A binder is attached to the copper film and binds to target analytes. A laser transmits a beam into the quartz prism at an incident angle that generates surface plasmons resonating with the copper film and reflects a phase shifted laser beam. Receive optics and a charge coupled device convert the phase shifted beam to electrical signals. A computing unit processes the electrical signals to compute refractive index changes based on phase shifts to indicate target analyte detection.

The invention belongs to the technical field of surface plasma sensing material preparation and biomedical detection, discloses a bovine serum albumin surface plasma resonance sensor for enhancing sensitivity based on a synthetic hydrotalcite material, and establishes a surface plasma resonance detection method. The preparation method comprises the following steps: firstly, sequentially plating a chromium film and a gold film on a slide by a vacuum coating method, and then covering and synthesizing hydrotalcite (hydrated aluminate magnesium carbonate) particles to modify the surface plasma resonance sensing chip by a spin-coating method. The surface plasmon formant of the SPR sensor modified by 1% hydrotalcite is from 637.9020 nm to 698.1382 nm, the variation amplitude is 60.2362 nm, which is far greater than the deviation amplitude of the formant of 14.98 nm of the currently reported SPR sensor modified by graphene oxide, and the BSA (Bovine Serum Albumin) detection sensitivity is greatly enhanced. The preparation is simple, the cost is low, and the application prospect is relatively strong.

The invention provides a photoluminescence molecule enhanced metasurface plasma resonance sensor and a preparation method thereof, and belongs to the technical field of metasurface plasma resonance sensors. Based on the photo-crosslinking polysaccharide sensor, a double-antibody sandwich detection system is formed through the antibody marked by photoluminescence molecules, the signal intensity and specificity of the metasurface plasma resonance sensor are remarkably enhanced, and target protein with lower concentration can be detected. The sensor disclosed by the invention has wide application potential, and is suitable for early diagnosis and monitoring of various diseases, especially occasions requiring high sensitivity and specificity; in addition, according to the sensor, the traditional signal amplification step is simplified, and the sensor has the advantages of being easy to operate and low in cost.

To provide a surface plasmon resonance sensor capable of obtaining a clear surface plasmon resonance response. [Solution] The surface plasmon resonance sensor comprises a substrate having a first surface and a second surface opposite to the first surface, a metal film provided on the first surface of the substrate and having a sample placement area on a third surface opposite to the first surface where a sample is placed, and being irradiated with light from the second surface side through the substrate, and a diffraction grating provided on the first surface or the second surface, such that at least a part of it overlaps with at least a part of the sample placement area when viewed in the thickness direction of the substrate, wherein the thickness of the substrate between the first surface and the second surface is t, and the refractive index of the substrate at the wavelength of the light is n, then t / n is 2 μm or less.

The invention belongs to the technical field of sensors, and particularly relates to a surface plasmon resonance sensor based on a molecularly imprinted polymer and a preparation method of the surface plasmon resonance sensor. The preparation method comprises the following steps: preparing an SPR chip with an Au / Cr / cover glass structure by adopting a magnetron sputtering method; then polydopamine is electrically polymerized on the surface to form a PDA / Au / Cr / cover glass structure; the preparation method comprises the following steps: preparing a dextromethorphan molecularly imprinted polymer (DXM-MIP) by taking p-mercaptophenol as a functional monomer and dextromethorphan as a template molecule through an electropolymerization method to form a DXM-MIP / PDA / Au / Cr / cover glass structure, and finally eluting the template molecule to obtain the surface plasmon resonance sensor (DXM-MIP / SPR sensor) based on the molecularly imprinted polymer. The DXM-MIP / SPR sensor is suitable for high-selectivity, wide-range, efficient and low-cost detection of dextromethorphan, and is especially suitable for qualitative and quantitative detection of dextromethorphan in drugs.

A photonic crystal fiber-based surface plasmon resonance (PCF-SPR) sensor to detect a refractive index of an analyte includes a fiber core having a first scale-down (SCD) cavity having a first diameter, multiple second SCD cavities each having a second diameter, multiple third SCD cavities each having a third diameter, and a groove. A surface of the groove is coated with a metal having a first thickness. The sensor includes an analyte channel having a second thickness and is in contact with the metal. The analyte channel surrounds the fiber core and is configured to stream the analyte. The sensor further includes an outer layer surrounding the analyte channel. The refractive index of the analyte is detected based on an intensity lost by an incident light passing through the PCF-SPR sensor due to dissipation of plasmonic energy.

The present invention is directed to an improved surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) sensors, improved SPR and LSPR sensor surface chemistry, and methods and systems for improved detection of analytes in SPR and LSPR. Use of the SPR and LSPR sensors described herein improves signal amplification, and thus, provides for higher signal-to-noise, in detection of various analytes, such as proteins, antibodies, carbohydrates, and nucleic acid molecules.

The present invention relates to the field of detection of the content of acidic proline-rich protein in children's saliva, and specifically discloses a composition for detecting the content of PRB1 in children's saliva and its application. The composition provided by the present invention contains TFIIB or a biotin-labeled TFIIB fusion protein. Using the surface plasmon resonance sensor detection method, it can not only effectively detect the content of PRB1 in children's saliva, but also effectively distinguish PRB1 from the other three saliva proline-rich proteins. Moreover, the detection sensitivity, accuracy and reproducibility of the content of PRB1 in children's saliva are higher than those of existing kits, providing solid technical support for predicting and diagnosing the symptoms of dental caries in children.

The present invention discloses a solution-interface measuring device based on the nano-slit confinement effect, a preparation method and a measuring method. The measuring device includes: a sample preparation layer, which includes a silicon substrate, on which two parallel electrodes spaced apart based on a MEMS process are formed, and a thiol self-assembly modification layer is formed on the upper surface of the electrode; a nano-groove is formed between the two electrodes, the entrance of the nano-groove is provided with an inclined introduction surface, and the surface of the inclined introduction surface is formed with a hydrophilic layer; an online monitoring layer, which includes a temperature control module, and the thermistor lattice of the temperature control module is arranged at the bottom of the silicon substrate; a fiber optic surface plasmon resonance sensor is also provided on the upper surface of the electrode, and a pH fiber optic probe is provided on the sidewall of the nano-groove; a signal processing layer, which includes an electrochemical impedance spectroscopy circuit board provided at the bottom of the online monitoring layer. The measuring device of the present invention can solve the problems of complex structure, easy clogging, weak interface signal and uneven solution introduction of traditional measuring devices.

The invention provides a supersurface SPR (Surface Plasmon Resonance) chip with a nanosphere array as well as a preparation method and application of the supersurface SPR chip, and belongs to the technical field of surface plasmon resonance sensors. The supersurface SPR chip with the nanosphere array comprises a substrate and a nanosphere array structure located on the substrate, and a metal film is deposited on the surface of the nanosphere array structure. The nanosphere array structure is reasonably designed on the surface of the chip, and surface chemical modification is optimized, so that natural conformation and functions of biomolecules can be better reserved, non-specific binding is reduced, the surface plasmon resonance effect is remarkably enhanced, the detection sensitivity is improved, and a powerful tool is provided for related research of biomolecules or cells.

This invention provides a surface plasmon resonance sensor and its applications, relating to the field of microwave sensor technology. The surface plasmon resonance sensor of this invention includes a dielectric substrate, a metallic waveguide, and a subwavelength resonator, wherein the subwavelength resonator has a fan-shaped defect structure. By introducing an asymmetric loss structure through the design of the subwavelength resonator with the fan-shaped defect structure, the surface plasmon resonance sensor of this invention achieves a singularity state, exhibiting a square-root-dependent high sensitivity response to external perturbations. This significantly improves detection sensitivity and enables high sensitivity and low detection limits for substances such as deionized water, glycerol solution, and glucose solution, as well as dynamic detection of the hydrolysis process of thioacetylcholine. It has advantages such as simple structure, easy integration, and high sensitivity.

This invention belongs to the field of sensor technology, specifically relating to a surface plasmon resonance sensor based on molecularly imprinted polymers and its fabrication method. The invention employs magnetron sputtering to fabricate an Au / Cr / cover glass structure SPR chip; then, polydopamine is electropolymerized on the surface to form a PDA / Au / Cr / cover glass structure; next, using p-mercaptophenol as a functional monomer and dextromethorphan as a template molecule, a dextromethorphan molecularly imprinted polymer (DXM-MIP) is prepared by electropolymerization, forming a DXM-MIP / PDA / Au / Cr / cover glass structure. Finally, the template molecule is eluted to obtain a surface plasmon resonance sensor based on molecularly imprinted polymers (DXM-MIP / SPR sensor). The DXM-MIP / SPR sensor is suitable for the highly selective, wide-range, efficient, and low-cost detection of dextromethorphan, especially for the qualitative and quantitative detection of dextromethorphan in pharmaceuticals.

The invention discloses a surface plasma resonance sensing chip and application thereof in detection of malachite green. The invention finds that the refractive index sensitivity of the SPR sensing chip can be effectively improved by regulating and controlling the deposition thickness of the ZIF-67 on the noble metal film, so that the refractive index sensitivity of the SPR sensing chip can be up to 2570.94 nm / RIU, which is improved by 42.30% compared with that of a pure noble metal film. In addition, the SPR sensing chip disclosed by the invention can be used for specifically detecting malachite green, and the surface refractive index of the SPR sensing chip is remarkably changed due to the interaction between ZIF-67 and malachite green. The preparation method of the SPR sensing chip is simple and easy to operate, and the prepared SPR sensing chip is high in sensitivity and good in linear correlation degree and has wide application prospects in the field of biological environment detection.