Method for raising surface plasma resonance sensor sensitivity by using nano gold grain catalytic growing

A surface plasmon and resonance sensor technology, applied in the field of improving the sensitivity of biosensors, can solve the problems of difficult and effective signal extraction, background increase, etc., and achieve the effect of low cost, high sensitivity and high sensitivity

Inactive Publication Date: 2006-08-09
HUNAN UNIV
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  • Application Information

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Problems solved by technology

In the process of catalytic growth of gold nanoparticles, gold or silver ions are catalytically reduced and deposited on the surface of gold nanoparticles, and at the same time, they will also be deposited on the surface of the metal film, causing the background to increase and it is difficult to extract effective signals from the background.

Method used

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  • Method for raising surface plasma resonance sensor sensitivity by using nano gold grain catalytic growing
  • Method for raising surface plasma resonance sensor sensitivity by using nano gold grain catalytic growing
  • Method for raising surface plasma resonance sensor sensitivity by using nano gold grain catalytic growing

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Embodiment 1

[0028] The surface plasmon resonance (SPR) sensor enhanced by nano-gold catalytic growth was used to detect 33nmol / L target DNA.

[0029] like figure 2 Shown, concrete steps of the present invention are:

[0030]1) Evaporate or sputter a layer of silicon dioxide (SiO2) with a thickness of 25 nm on the surface of the gold film 1 2 ) thin layer 2, made of silicon dioxide (SiO 2 ) The gold film 1 coated with the thin layer 2 is used as the metal film of the surface plasmon resonance (SPR) sensor, and the following reaction process is monitored with the surface plasmon resonance (SPR) sensor.

[0031] 2) By physical adsorption method, on silicon dioxide (SiO 2 ) thin layer 2 coated gold film 1 surface adsorption biotinylated bovine serum albumin (BSA), then add avidin and biotinylated bovine serum albumin (BSA) in combination to obtain a surface rich in avidin Silicon dioxide (SiO 2 ) gold film coated with a thin layer; through the binding between biotin-avidin, the biotin-l...

Embodiment 2

[0034] A surface plasmon resonance (SPR) sensor enhanced by catalytic growth of gold nanoparticles was used to detect different concentrations of target DNA.

[0035] The specific steps are:

[0036] 1), adopt the method for vacuum coating, in gold film 1 (referring to figure 2 ) surface evaporated or sputtered a layer of 25nm thick silicon dioxide (SiO 2 ) thin layer 2, which consists of silicon dioxide SiO 2 The gold film 1 coated with a thin layer is used as the metal film of the surface plasmon resonance SPR sensor.

[0037] 2), through the method of physical adsorption, in silicon dioxide (SiO 2 ) thin layer 2 coated gold film 1 surface adsorption biotinylated bovine serum albumin (BSA), then add avidin and biotinylated bovine serum albumin (BSA) in combination to obtain a surface rich in avidin Silicon dioxide (SiO 2 ) gold film coated with a thin layer; through the binding between biotin-avidin, the biotin-labeled DNA probe A3 was immobilized on the metal film of ...

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Abstract

Present invention discloses a method utilizes nano gold particle catalytic rising to raise surface plasma resonance transducer sensitivity. It coats 20-35 nm silicon dioxide thin layer on plasma resonance transducer metallic film surface, utilizes nano gold particle catalyzing to enhance surface plasma resonance signal and to raise plasma SPR transducer sensitivity. Said invention makes nano gold particle catalyzing rising in surface plasma resonance SPR transducer, in nano gold particle catalyzing process, gold or silver ion depositing on nano gold particle surface by catalytic reduction not depositing on metallic film surface to result in background heightening. Said method has simple, easy operation and high sensitivity.

Description

technical field [0001] The invention belongs to a method for improving the sensitivity of a biosensor, in particular to a method for improving the sensitivity of a surface plasmon resonance (Surface Plsamon Resonance, abbreviated as SPR) sensor by utilizing the catalytic growth of nano-gold particles. Background technique [0002] Nano-gold particles refer to gold particles with a particle diameter between 1 and 100 nm. It is often applied with gold sol formed by dispersing gold particles in water, so it is also called colloidal gold. Gold nanoparticles are mesoscopic particles with a special electronic structure, exhibiting many unique optical and electrical properties, and have been widely used in materials science, clinical medicine, life science and other fields. For example, gold nanoparticles of different sizes are used in the array chip to achieve multicolor labeling; another example is to establish an optical method for detecting specific oligonucleotide sequences b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/55G01N21/27G01N21/17G01N33/48H01L49/00G01N21/552
Inventor 王柯敏羊小海王青
Owner HUNAN UNIV
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