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Kit for fluorescence quantitative detection of salbutamol and preparation method of fluorescence labeling liquid

A fluorescent quantitative detection and albuterol technology, applied in the field of medical testing, can solve problems such as unfavorable on-site testing, and achieve the effects of simple operation, high sensitivity and high sensitivity

Inactive Publication Date: 2012-03-28
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kit has the characteristics of high sensitivity, good repeatability, simplicity, speed, and accuracy. Compared with the traditional colorimetric ELISA method, the sensitivity can be increased by an order of magnitude, and it is expected to be used in animal-derived foods (such as milk samples, animal tissue samples) and urine. It plays an important role in the detection of salbutamol residues in samples. However, this method does not require a long reaction time, which is not conducive to on-site testing.

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  • Kit for fluorescence quantitative detection of salbutamol and preparation method of fluorescence labeling liquid
  • Kit for fluorescence quantitative detection of salbutamol and preparation method of fluorescence labeling liquid
  • Kit for fluorescence quantitative detection of salbutamol and preparation method of fluorescence labeling liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0032] In this embodiment, the immunochromatographic kit for the fluorescent quantitative detection of albuterol includes a test strip and a fluorescent labeling solution.

[0033] Wherein, according to the conventional method of the test strip, the test strip consists of a sample pad 1, a nitrocellulose coating 2 including a detection area (T area) 3 and a quality control area (C area) 4, and an absorbent paper 5 on the base plate 6. The composition is successively overlapped and pasted together, such as figure 1 and figure 2 shown.

[0034]In this embodiment, 0.2 mg / ml SAL-BSA conjugate (salbutamol antigen) coating solution was used at the T line of the detection area of ​​the coated membrane, and the usage amount was 90 ul / 27 cm. Anti-rabbit IgG with a concentration of 0.2mg / ml was used to coat the C line of the quality control area of ​​the coated membrane, and the amount used was the same as 90ul / 27cm. Used to bind fluorescently labeled rabbit IgG to test the validity...

Embodiment 2

[0039] In this embodiment, the fluorescent quantitative detection salbutamol immunochromatographic kit includes a test strip and a fluorescent labeling solution.

[0040] Wherein, the test strip consists of a sample pad, a cellulose-coated film including a detection area (T area) and a quality control area (C area), and absorbent paper, which are successively pasted on the base plate by overlapping each other according to the conventional method of the test paper.

[0041] In this embodiment, 1.5 mg / ml SAL-BSA conjugate (salbutamol antigen) coating solution was used at the T line of the detection area of ​​the coated membrane, and the usage amount was 90 ul / 35 cm. Use anti-rabbit IgG with a concentration of 0.5mg / ml at the C line of the quality control area of ​​the coating membrane, and the amount used is the same as 90ul / 35cm, which is used to bind fluorescently labeled rabbit IgG and to detect the effectiveness of the test strip. sex.

[0042] In this embodiment, after the...

Embodiment 3

[0046] In this embodiment, the fluorescent quantitative detection salbutamol immunochromatographic kit includes a test strip and a fluorescent labeling solution.

[0047] Wherein, the test strip consists of a sample pad, a cellulose-coated film including a detection area (T area) and a quality control area (C area), and absorbent paper, which are successively pasted on the base plate by overlapping each other according to the conventional method of the test paper.

[0048] In this embodiment, 1.0 mg / ml SAL-BSA conjugate (salbutamol antigen) coating solution was used at the T line of the detection area of ​​the coated membrane, and the usage amount was 90 ul / 35 cm. Anti-rabbit IgG with a concentration of 0.5mg / ml was used to coat the C line of the quality control area of ​​the coated membrane, and the amount used was the same as 90ul / 35cm. Used to bind fluorescently labeled rabbit IgG to test the validity of test strips.

[0049] In this embodiment, the fluorescent labeling li...

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Abstract

The invention discloses an immunochromatography kit for fluorescence quantitative detection of salbutamol and a preparation method of a fluorescence labeling liquid. The kit comprises a test strip and a fluorescence labeling liquid, wherein the test strip is formed by sequentially lapping and sticking a sample pad, a nitrocellulose coating film and absorbent paper on a bottom plate; the nitrocellulose coating film comprises a detection area and a quality control area; the detection area is coated with an SAL-BSA conjugate; the quality control area is coated with anti-rabbit IgG; and the fluorescence labeling liquid contains a fluorescence labeling SAL antibody and a fluorescence labeling rabbit IgG. Compared with an immune colloidal gold labeling test strip, the kit disclosed by the invention has the advantages of higher sensitivity, accurate quantification and the like; and the operation is faster and more convenient than that of an enzyme coupling method.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to an immunochromatographic detection kit for fluorescent quantitative detection of albuterol (SAL) and a preparation method of a fluorescent labeling solution. Background technique [0002] Salbutamol, whose chemical name is 1-(4-hydroxy-3-hydroxymethylphenyl)-2-(tert-butylamino)ethanol, is a drug that can bind to adrenergic receptors and is mainly used clinically for the treatment of asthma , Bronchospasm, etc. The use of SAL as a feed additive can significantly promote animal growth, increase feed conversion rate and lean meat rate. If used in excess for a long time, it will accumulate in animal tissues, and people will be poisoned after eating this animal product. In the early 1990s, there were many stimulant poisoning incidents in Europe, and there have been many poisoning incidents in my country since 1998. Therefore, EU countries and my country have made corresponding regulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 王继华唐海波刘晓云
Owner GUANGZHOU WONDFO BIOTECH
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