The invention discloses an integral time constant calibration method which is characterized by comprising following steps: (1) a pulse signal with an amplitude of U is given to an input end of an integrator, and the integrator integrates the pulse signal to obtain an integral output curve; (2) according to the formula, after a time T, an integral output voltage is that V=(U/RC)T+U, wherein R is an integrator resistance, C is an integrator capacitance, T is an integral time, and U is an integrator initial voltage, thereby the integral slope K can be calculated by the integral output curve; (3) the slope of the integral output curve is that K=(V to U)/T=(U/RC), thereby an integral time constant is that t=RC=U/K, wherein U is the amplitude of the pulse signal, K is the slope of the integral output curve, U and K can be calculated by the formula, and the integral time constant t can be calculated by substituting U and K. The method of the invention has simple operation and clear steps, the according formulas are all known physical formulas, and no special devices are needed in the method of the invention. And the method of the invention has rapid measurement process and accurate measured value, and is convenient for technicist to use.