Preparation method for synthesizing polyphenol compound by using enzyme immobilization technology

A technology for immobilizing polyphenols and enzymes, applied in biochemical equipment and methods, immobilizing enzymes, immobilizing on/in organic carriers, etc., can solve the problem of low yield of polyphenols, and achieve The effect of inhibiting the formation of quinones, high catalytic performance, and improving yield

Pending Publication Date: 2020-02-21
SHENZHEN UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a preparation method for synthesizing polyphenolic compounds with enzyme immobilization technology. The problem that the existing production rate of polyphenolic compounds synthesized by tyrosinase is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for synthesizing polyphenol compound by using enzyme immobilization technology
  • Preparation method for synthesizing polyphenol compound by using enzyme immobilization technology
  • Preparation method for synthesizing polyphenol compound by using enzyme immobilization technology

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] see figure 1 , figure 1 A flow chart of a preferred embodiment of a preparation method for synthesizing polyphenolic compounds with enzyme immobilization technology provided by the present invention, as shown in the figure, it includes the following steps:

[0034] S10, mixing metal ions or metal clusters, organic ligands and tyrosinase solution, immobilizing tyrosinase on the metal-organic framework formed by combining metal ions or metal clusters with organic ligands to obtain tyrosinase- metal-organic framework complexes;

[0035] S20, adding the tyrosinase-metal-organic framework complex into the monophenolic compound solution, and adding a reducing agent, and reacting under predetermined reaction conditions to synthesize the polyphenolic compound.

[0036] Through the synthesis method provided in this example, better immobilization effect and catalytic performance of tyrosinase can be obtained, and the synthesis method is simple to operate and can effectively inc...

Embodiment 1

[0056] The effect of different conditions on the activity of TYR@ZIF-8.

[0057] To explore the effect of the concentration of 2-methylimidazole on the activity of the generated TYR@ZIF-8: prepare a total system of 200mL, 100mL tyrosinase solution, 80mL 2-methylimidazole solution of different concentrations and 20mL zinc acetate solution (0.4 M) Mix, stir at 4°C for 1 h, centrifuge the mixture (4°C, 8000 rpm, 10 min) and wash the precipitate several times with phosphate buffer (50 mM, pH 6.0), dry the precipitate in vacuum, and grind the sample to powder. Wherein the final concentration of 2-methylimidazole is 80mM, 160mM, 320mM, 640mM respectively, and the final concentration of zinc acetate is 40mM. The optimal concentration of 2-methylimidazole was obtained by measuring the enzyme activity of TYR@ZIF-8, wherein the method of enzyme activity determination was L-dopa as substrate, and the absorbance value at A475nm was measured by UV-Vis spectrophotometer , then according to...

Embodiment 2

[0062] HPLC detection spectrum of picatanol.

[0063] Shimadzu high performance liquid chromatograph, adopt reverse C18 post (Japan GLSciences, Inc company, InertsilODS-SP, 4.6 * 150mm, 5 μ m), this liquid condition is: mobile phase A is 0.5% acetic acid / acetonitrile=95 / 5 (v / v), the mobile phase B is acetonitrile / 0.5% acetic acid=95 / 5(v / v), A / B=75 / 25(v / v), the injection volume is 10μL, and the total flow rate is 1.0mL / min , the detection wavelength is 320nm, and the column temperature is 29°C.

[0064] Using 50% acetonitrile (v / v) to accurately prepare picetanol standard solutions with serial concentrations, and detect the peak pattern of picetanol by HPLC. The picatatanol produced by TYR@ZIF-8 catalysis was properly diluted and analyzed qualitatively and quantitatively by HPLC. The HPLC detection spectrum of picatanol is as follows image 3 .

[0065] Such as image 3 As shown, the peak time of the product produced by TYR@ZIF-8 catalyzing resveratrol was consistent with ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method for synthesizing a polyphenol compound by using an enzyme immobilization technology. The preparation method comprises the following steps: mixing metal ionsor metal clusters with an organic ligand and tyrosinase, and immobilizing the tyrosinase to obtain a tyrosinase-metal organic framework compound; and using the tyrosinase-metal organic framework compound as a catalyst and a monophenol compound as a reactant so as to synthesize the polyphenol compound. A metal organic framework is formed by coordination of the metal ions or the metal clusters andthe organic ligand under the induction of the tyrosinase. Meanwhile, the metal organic framework forms a tyrosinase protective shell, so that the tyrosinase is immobilized, and the obtained immobilized tyrosinase has relatively high catalytic performance. Monophenol compounds are catalyzed by the immobilized tyrosinase to synthesize an ortho-hydroxylated polyphenol compound, and a reducing agent is added to inhibit the polyphenol compound from being further oxidized, so that the yield of the polyphenol compound is increased.

Description

technical field [0001] The invention relates to the technical field of enzyme immobilization and enzyme catalysis, in particular to a preparation method for synthesizing polyphenolic compounds with enzyme immobilization technology. Background technique [0002] Polyphenolic compounds refer to substances with two or more hydroxyl groups on the benzene ring. Its unique polyhydric structure makes its properties better than monophenolic compounds. The effects of reducing blood fat and enhancing immune function are of great significance to human health, and have great application and research value in the fields of biomedicine and health care. [0003] At present, polyphenolic compounds are mostly synthesized by chemical and biological methods. Compared with chemical methods, biological methods use enzymes to catalyze the synthesis of polyphenolic compounds, which has the advantages of high specificity, high efficiency, environmental protection, and easy recovery. It has receive...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/089C12N9/02C12P7/22C12P13/22
CPCC12N11/08C12N9/0059C12Y110/03001C12P7/22C12P13/225
Inventor 杨缜韦春媚
Owner SHENZHEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap