Method for removing phenolic substances in phenol aqueous solution by tyrosinase-metal organic framework compound

A metal-organic framework, tyrosinase technology, applied in the direction of immobilization on/in organic carriers, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem of low efficiency of phenolic substances, phenol removal technology Complex and other problems, to achieve the effect of efficient removal

Inactive Publication Date: 2020-12-08
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for removing phenolic substances in phenolic aqueous solution with a tyrosinase-metal organic framework complex, aiming at solving the complex process of the existing phenol removal technology and the treatment of phenolic substances. The problem of low material efficiency

Method used

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  • Method for removing phenolic substances in phenol aqueous solution by tyrosinase-metal organic framework compound
  • Method for removing phenolic substances in phenol aqueous solution by tyrosinase-metal organic framework compound
  • Method for removing phenolic substances in phenol aqueous solution by tyrosinase-metal organic framework compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Exploring the effect of tyrosinase-metal-organic framework complex on the scavenging effect of different concentrations of phenol

[0060] Weigh 15 mg of tyrosinase-metal-organic framework complex into a 50 mL centrifuge tube, and then mix with the 10mL of phenol solution was reacted, reacted in a constant temperature shaker (30°C, 250rpm) for 2h, then sampled, analyzed by HPLC and calculated the clearance rate of different phenol concentrations, and each concentration made 3 parallel groups. The result is as figure 2 shown.

[0061] It can be seen from the results that the scavenging effect of tyrosinase-MOF complex on phenol solution decreases with the increase of phenol concentration, and the scavenging effect is better at low phenol concentration, but the scavenging effect is limited at high phenol concentration.

Embodiment 2

[0063] Exploring the effect of different temperatures on phenol scavenging by tyrosinase-metal-organic framework complexes

[0064] Weigh 30mg of tyrosinase-metal organic framework complex into a 50mL centrifuge tube, add 20mL, 2.5mmol / L phenol solution, and then ℃) constant temperature shaker (250rpm) co-reaction for 2h, sampling every 0.5h, using HPLC detection and analysis and calculation of phenol removal rate at different temperatures, each temperature made a total of 3 parallel groups. The result is as image 3 shown.

[0065] From the results, it can be seen that the effect of 20°C to 60°C on the removal of phenol by the tyrosinase-metal organic framework complex shows that the removal efficiency is the best in the range of 25°C to 30°C, and when it is higher than 40°C, high temperature leads to enzyme inactivation , the scavenging efficiency becomes lower, and 30°C is selected as the optimum temperature for tyrosinase-MOF complex to scavenge phenol.

Embodiment 3

[0067]Exploring the effect of different pH on phenol scavenging by tyrosinase-metal-organic framework complex

[0068] Weigh 35mg of tyrosinase-metal organic framework complex into a 50mL centrifuge tube, add 20mL, 2.5mmol / L phenol solution configured with different pH (5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0), and then The co-reaction was carried out on a constant temperature shaker (30°C, 250rpm) for 2 hours, and samples were taken every 0.5 hours. The clearance rate of phenol at different pH was calculated by HPLC, and 3 parallel groups were made for each pH. The result is as Figure 4 shown.

[0069] From the results, it can be seen that pH 5.0-8.0 has little effect on phenol scavenging effect of tyrosinase-metal-organic framework complex. The scavenging efficiency decreased slightly, and pH 6.0 was chosen as the optimum pH for tyrosinase-MOF complex to scavenge phenol.

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Abstract

The invention discloses a method for removing phenolic substances in a phenol aqueous solution by using a tyrosinase-metal organic framework compound. The tyrosinase-metal organic framework compound is added into the phenol aqueous solution to react with the phenolic substances so as to achieve the purpose of phenol removal; the tyrosinase-metal organic framework compound is prepared by the following method: mixing metal ions or metal clusters, organic ligands and a tyrosinase solution so that tyrosinase is immobilized on a porous hybrid metal-organic framework generated by the metal ions or the metal clusters and the organic ligands through coordination; the tyrosinase-metal organic framework compound is obtained. The tyrosinase-metal organic framework compound is simple in preparation process, low in cost, environmentally friendly and capable of efficiently removing the phenolic substances in water.

Description

technical field [0001] The invention relates to the technical field of phenol removal, in particular to a method for removing phenolic substances in phenol aqueous solution by a tyrosinase-metal organic framework compound. Background technique [0002] Phenol pollution in the environment mainly refers to the pollution of water by phenolic compounds. Phenol-containing wastewater is one of the most harmful and widely polluting industrial wastewater in the world today, and is an important source of water pollution in the environment. Phenolic compounds are highly toxic pollutants even at low concentrations. The phenolic wastewater with high toxicity and refractory degradation will deteriorate the water quality and seriously threaten the health of humans and other organisms if discharged without treatment. [0003] At present, the treatment methods of phenolic substances in wastewater mainly include chemical methods, physical methods, and biological methods. The physical treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N11/02C12N9/02C02F101/34
CPCC02F3/342C12N11/02C12N9/0059C12Y110/03001C02F2101/345
Inventor 杨缜韦春媚
Owner SHENZHEN UNIV
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