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Method for refining raw oil by using magnetic immobilized phospholipase A1

A technology of immobilizing phospholipase and crude oil, which is applied in the direction of fat oil/fat refining and fat production, can solve the problems of complex separation and recovery of immobilized enzymes and low activity recovery rate, and achieve improved reusability, higher recovery rate, The effect of improving usage efficiency

Inactive Publication Date: 2012-07-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to prepare immobilized phospholipase A in order to solve existing method 1 There is a problem of complex separation and recovery of immobilized enzymes, low recovery rate of activity, and the problem that magnetic immobilized enzymes are not used for refining crude oil at present, and a magnetically immobilized phospholipase A is provided 1 Preparation method of and magnetically immobilized phospholipase A 1 The method of refining crude oil

Method used

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specific Embodiment approach 1

[0016] Specific embodiments 1. The magnetically immobilized phospholipase A of the present embodiment 1 The preparation method, carries out according to the following steps: one, prepare Fe 3 o 4 Nanoparticles; 2. Preparation of Fe 3 o 4 / SiO x Composite particles; 3. Preparation of Fe 3 o 4 / SiO x -APTS carrier; four, preparation of magnetic Fe 3 o 4 / SiO x -g-P (GMA) composite particles; five, the magnetic Fe 3 o 4 / SiO x -g-P (GMA) composite particles are added to 0.1mol / L and the pH value is 7~7.2 in the phosphate buffer solution soaked 24~26h, then carry out magnetic separation, obtain the carrier after soaking; Sixth, the phospholipase A 1 Add 0.1mol / L to phosphate buffer solution with a pH value of 5.5-9.5 to make phospholipase A with a mass concentration of 0.2% 1 Phosphate buffer solution, then add the soaked carrier to 12-88mL phospholipase A 1 In the phosphate buffer, stir with a magnetic stirrer at 10-50°C for 2-9 hours, the stirring speed is 10-12r / m...

specific Embodiment approach 2

[0017] Specific embodiment two: the difference between this embodiment and specific embodiment one is that Fe is prepared in step one 3 o 4 The concrete method of nanoparticle is: a, the FeCl of 1mol / L 2 solution and 1.75mol / L FeCl 3 The solution is mixed so that the Fe 2+ with Fe 3+ The molar ratio is 1: 1.5, and under the protection of nitrogen, stir in a water bath at 60°C, the stirring speed is 10-40r / min, while adding dropwise the ammoniacal liquor with a mass concentration of 25%-28% to adjust the pH value to 10, and then heat up to Keep at 80°C for 1 hour, then cool to room temperature to obtain product A; b. Separate product A with a permanent magnet, wash with distilled water until the pH value is neutral, and vacuum dry at room temperature for 24 hours to obtain Fe 3 o 4 Nanoparticles. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0018] Specific embodiment three: the difference between this embodiment and specific embodiment one is that Fe is prepared in step two 3 o 4 / SiO x The concrete method of compound particle is: a, take by weighing 2gFe 3 o 4 Nanoparticles, add 160mL ethanol, 40mL water and 5mL ammonia water with a mass concentration of 25%, disperse with 20-40KHz ultrasonic waves for 1 hour, stir at a stirring speed of 10-50r / min, and drop 10-50mL of orthosilicon Acetate ethyl ester, after reacting for 12 hours, product B was obtained; b. Separate product B with a permanent magnet, wash with distilled water until the pH value was neutral, then cover product B with 1mol / L hydrochloric acid, soak for 12 hours, and magnetically separate Afterwards, wash with distilled water until the pH value is neutral, and vacuum-dry at room temperature for 24 hours to obtain Fe 3 o 4 / SiO x Composite particles. Others are the same as in the first embodiment.

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Abstract

The invention provides a method for refining raw oil by using magnetic immobilized phospholipase A1, which relates to a method for refining raw oil by using immobilized phospholipase A1. The invention aims to solve the problems that the separation and the recovery of an immobilized enzyme are complicated, the activity recovering rate is low and a magnetic immobilized enzyme is not used for refining raw oil at present, which exist in a traditional method for preparing immobilized phospholipase A1. The method for preparing and refining the raw oil comprises the following steps of: (1) preparingthe magnetic immobilized phospholipase A1; (2) heating the raw oil and adding a citric acid solution; and (3) cooling, regulating the pH value, then adding the magnetic immobilized phospholipase A1, stirring and separating the magnetic immobilized phospholipase A1 so as to finish the preparation method of the magnetic immobilized phospholipase A1 and the method for refining the raw oil. The recovering rate of the magnetic immobilized phospholipase A1 prepared by the method for refining the raw oil by using the magnetic immobilized phospholipase A1 reaches more than 85 percent, so that the phosphorus content of the raw oil is lowered to 7-10mg / kg. The method for refining the raw oil by using the magnetic immobilized phospholipase A1 is applied to the field of degumming of grease.

Description

[0001] The patent application of the present invention is the application number 201010538226.9, the application date is November 10, 2010, and the invention name is "Magnetic immobilized phospholipase A 1 Preparation method of and magnetically immobilized phospholipase A 1 A divisional application for the invention patent of "method for refining crude oil". technical field [0002] The present invention relates to a kind of using immobilized phospholipase A 1 The method of refining crude oil. Background technique [0003] While maintaining its high-efficiency, specificity and mild enzyme-catalyzed reaction characteristics, the immobilized enzyme overcomes the shortcomings of free enzymes, showing high storage stability, easy separation and recovery, repeated use, continuous and controllable operation, The process is simple and other advantages. Immobilized enzymes are widely used in the fields of chemistry, biology and bioengineering, medicine and life sciences. Prepara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00
Inventor 于殿宇王立琦江连洲朱秀清王玉于坤弘常云鹤孙博刘晶周晓丹王妍陈晓慧
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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