Method for preparing magnetic immobilized phospholipase A1 and method for refining crude oil by using magnetic immobilized phospholipase A1

An immobilized phospholipase and magnetic technology, which is applied in the direction of immobilization on or in the inorganic carrier, can solve the problems of complex separation and recovery of immobilized enzymes, low activity recovery rate, etc., to achieve large-scale continuous operation and improve activity The recovery rate and the effect of improving the catalytic efficiency

Inactive Publication Date: 2011-05-18
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to prepare immobilized phospholipase A in order to solve existing method 1 There is a problem of complex separation and recovery of immobilized enzymes, low recovery rate of activity, and the problem that magnetic immobilized enzymes are not used for refining crude oil at present, and a magnetically immobilized phospholipase A is provided 1 Preparation method of and magnetically immobilized phospholipase A 1 The method of refining crude oil

Method used

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specific Embodiment approach 1

[0015] Specific embodiments 1. The magnetically immobilized phospholipase A of the present embodiment 1 The preparation method, carries out according to the following steps: one, prepare Fe 3 o 4 Nanoparticles; 2. Preparation of Fe 3 o 4 / SiO x Composite particles; 3. Preparation of Fe 3 o 4 / SiO x -APTS carrier; four, preparation of magnetic Fe 3 o 4 / SiO x -g-P (GMA) composite particles; five, the magnetic Fe 3 o 4 / SiO x -g-P (GMA) composite particles are added to 0.1mol / L and the pH value is 7~7.2 in the phosphate buffer solution soaked 24~26h, then carry out magnetic separation, obtain the carrier after soaking; Sixth, the phospholipase A 1 Add 0.1mol / L to phosphate buffer solution with a pH value of 5.5-9.5 to make phospholipase A with a mass concentration of 0.2% 1 Phosphate buffer solution, then add the soaked carrier to 12-88mL phospholipase A 1 In the phosphate buffer, stir with a magnetic stirrer at 10-50°C for 2-9 hours, the stirring speed is 10-12r / m...

specific Embodiment approach 2

[0016] Specific embodiment two: the difference between this embodiment and specific embodiment one is that Fe is prepared in step one 3 o 4 The concrete method of nanoparticle is: a, the FeCl of 1mol / L 2 solution and 1.75mol / L FeCl 3 The solution is mixed so that the Fe 2+ with Fe 3+ The molar ratio is 1: 1.5, and under the protection of nitrogen, stir in a water bath at 60°C, the stirring speed is 10-40r / min, while adding dropwise the ammoniacal liquor with a mass concentration of 25%-28% to adjust the pH value to 10, and then heat up to Keep at 80°C for 1 hour, then cool to room temperature to obtain product A; b. Separate product A with a permanent magnet, wash with distilled water until the pH value is neutral, and vacuum dry at room temperature for 24 hours to obtain Fe 3 o 4 Nanoparticles. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0017] Specific embodiment three: the difference between this embodiment and specific embodiment one is that Fe is prepared in step two 3 o 4 / SiO x The concrete method of compound particle is: a, take by weighing 2gFe 3 o 4 Nanoparticles, add 160mL ethanol, 40mL water and 5mL ammonia water with a mass concentration of 25%, disperse with 20-40KHz ultrasonic waves for 1h, stir at a stirring speed of 10-50r / min, and drop 10-50mL of orthosilicon Acetate ethyl ester, after reacting for 12 hours, product B was obtained; b, the product B was separated with a permanent magnet, washed with distilled water until the pH value was neutral, and then the product B was covered with 1mol / L hydrochloric acid, soaked for 12 hours, and magnetically separated Afterwards, wash with distilled water until the pH value is neutral, and vacuum-dry at room temperature for 24 hours to obtain Fe 3 o 4 / SiO x Composite particles. Others are the same as in the first embodiment.

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Abstract

The invention relates to a method for preparing magnetic immobilized phospholipase A1 and a method for refining crude oil by using the magnetic immobilized phospholipase A1. The invention aims to solve the problems of complicated separation and recycling of immobilized enzyme and low activity recovery of the conventional method for preparing the immobilized phospholipase A1, and the problem that the magnetic immobilized enzyme is not used for refining the crude oil at present. The method for preparing and refining the crude oil comprises the following steps of: 1, preparing the magnetic immobilized phospholipase A1; 2, heating the crude oil, and adding citric acid solution; and 3, cooling, regulating the pH value, adding the magnetic immobilized phospholipase A1, stirring, and separating the magnetic immobilized phospholipase A1. The recovery rate of the prepared magnetic immobilized phospholipase A1 is over 85 percent, and the phosphorus content of the crude oil is reduced to 7-10mg/kg. The invention is applied to the field of oil degumming.

Description

technical field [0001] The invention relates to an immobilized phospholipase A 1 Preparation method and immobilized phospholipase A 1 The method of refining crude oil. Background technique [0002] While maintaining its high-efficiency, specificity and mild enzyme-catalyzed reaction characteristics, the immobilized enzyme overcomes the shortcomings of free enzymes, showing high storage stability, easy separation and recovery, repeated use, continuous and controllable operation, The process is simple and other advantages. Immobilized enzymes are widely used in the fields of chemistry, biology and bioengineering, medicine and life sciences. Preparation of immobilized phospholipase A using existing methods 1 It is difficult to separate and recover the immobilized enzyme, and the activity recovery rate is low (40% to 60% in general). [0003] The colloid in vegetable oil is mainly a mixture of phospholipids. Degumming is a very important process in vegetable oil refining. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12P7/64
Inventor 于殿宇王立琦江连洲朱秀清王玉于坤弘常云鹤孙博刘晶周晓丹王妍陈晓慧
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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