Catalase immobilization method on basis of using amidoxime PAN nanofibrous membrane as carrier

A nanofiber membrane, catalase technology, which is applied in the direction of being fixed on/in an organic carrier, can solve the problems of low binding force between the enzyme and the carrier, difficult to reuse, and reduced enzyme activity, and achieve the recovery rate of activity. The effect of high, high load capacity and good bioaffinity

Inactive Publication Date: 2011-06-22
JIANGNAN UNIV
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the adsorption method is easy to operate and has little effect on enzyme activity, but the binding force between the enzyme and the carrier is low, making it difficult to reuse; while immobilization through covalent bonds has a strong binding force, but has a serious impact on the conformation of the enzyme , greatly reducing the activity of the enzyme

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Catalase immobilization method on basis of using amidoxime PAN nanofibrous membrane as carrier
  • Catalase immobilization method on basis of using amidoxime PAN nanofibrous membrane as carrier
  • Catalase immobilization method on basis of using amidoxime PAN nanofibrous membrane as carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] 2 g of PAN with a weight average molecular weight of 79100 was dissolved in N,N-dimethylformamide to prepare 20 mL of a 10% by mass spinning solution, which was placed in a self-made electrospinning device. The parameters of the electrospinning process are: the distance between the spinneret and the receiving plate is 17cm, the flow rate of the spinning solution is 0.5mL / min, the diameter of the spinneret is 0.7mm, the static voltage of 18KV is applied, and the receiving time is 22 hours through aluminum foil. Then dry in a vacuum oven for 2 hours to remove the remaining solvent to obtain a PAN nanofiber film.

[0020] Configure a 0.5mol / L hydroxylamine hydrochloride solution, adjust the pH value to 6.0 with sodium carbonate; soak 0.2 grams of electrospun PAN nanofibers in 50 mL of a hydroxylamine hydrochloride solution with a pH value of 6.0 and a concentration of 0.5mol / L for 120 minutes, and rinse with distilled water After washing 4 times, the amidoxime PAN nanofibe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A catalase immobilization method on the basis of using an amidoxime PAN nanofibrous membrane as carrier relates to the technical fields of nanofibrous membrane preparation utilizing electrostatic spinning technology, PAN functional modification and enzyme immobilization. The method comprises the following steps: the electrostatic spinning technology is utilized to obtain a PAN nanofibrous membrane, amidoximation modification is performed to the nanofibrous membrane to obtain the amidoxime PAN nanofibrous membrane with stable morphology, high porosity and strong load capacity and biocompatibility, then Cu<2+> is used as centre body, and catalase is immobilized on the amidoxime PAN nanofibrous membrane through coordinate bond. Compared with the conventional catalase immobilization method, the method of the invention solves the defects of catalase immobilization that the recovery rate of enzyme activity is low, the storage instability is low and the reusability is unstable. The immobilized catalase on the basis of using the amidoxime PAN nanofibrous membrane as carrier can be used in various fields such as biosensor preparation, membrane bioreactor and environmentally friendly material.

Description

technical field [0001] The invention relates to a method for immobilizing enzymes with an amidoxime PAN nanofiber membrane as a carrier, in particular to preparing a PAN nanofiber membrane by electrospinning, preparing an amidoxime PAN nanofiber membrane through amidoxime modification, and Using it as a novel enzyme immobilization carrier, the method for immobilizing catalase through metal coordination binding. technical background [0002] Enzyme is a biocatalyst with high catalytic efficiency and strong specificity (such as substrate specificity and stereoisomer specificity). However, due to the protein characteristics of enzymes, any factors that can cause protein denaturation may cause enzyme inactivation, such as pH, heavy metal salts, heat, ultraviolet rays, and violent shocks. Even under optimal catalytic conditions, the enzyme-catalyzed reaction rate will gradually decrease with the prolongation of the reaction time. In addition, free enzymes are difficult to recov...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08
Inventor 凤权魏取福黄锋林乔辉王清清
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap