Zwitter-ion magnetic composite hydrogel immobilized enzyme supporter and preparation method

Abstract

The invention relates to a zwitter-ion magnetic composite hydrogel immobilized enzyme supporter and a preparation method. The immobilized enzyme supporter is zwitter-ion hydrogel in which Fe3O4 nanoparticles are embedded; one end of glutaraldehyde, which has a bifunctional group, is connected to the zwitter-ion hydrogel, while the other end forms Schiff base with amino groups on the surface of theenzyme, so that the enzyme is covalently connected to the surface of the hydrogel supporter. The preparation method includes the steps of: mixing a zwitter-ion monomer, a crosslinking agent, an initiator and a solvent to obtain a liquid mixture, and mixing the liquid mixture with a Fe3O4 solution according to volume ratio of 3:5-3:10; and performing ultraviolet radiation, or heating or normal temperature treatment to the mixture solution containing Fe3O4 so that the Fe3O4 is embedded in the monomer via crosslinking polymerization, thereby forming the zwitter-ion magnetic composite hydrogel immobilized enzyme supporter in which the Fe3O4 is embedded. The enzyme immobilization method inherits the advantages in conventional covalent combination enzyme immobilization methods. The method is high in recovery rate of enzyme activity and has high application value.

Description

technical field [0001] The invention relates to a kind of zwitterionic magnetic composite hydrogel immobilized enzyme carrier embedded with ferric oxide. The invention relates to the preparation method and application of the carrier. More specifically, the present invention relates to a preparation method of a zwitterionic magnetic composite hydrogel immobilized enzyme carrier embedded with ferric oxide and the use of the carrier immobilized enzyme prepared by the method. Background technique [0002] Enzymes are an important class of biocatalysts, which can participate in various metabolic reactions in organisms, and have the advantages of mild action conditions, high efficiency, specificity, and wide sources. Enzymes are widely used in brewing, medicine, food processing, chemical analysis, environmental protection, energy development and other fields. However, most natural enzymes are proteins, and the high-level structure of protein enzymes is very sensitive to the envi...

AI Technical Summary

Problems solved by technology

[0004] Enzyme immobilization carriers have been widely used from the initial natural polymer materials to later synthetic polymer materials, inorganic materials, and now composite materials, but still have more or less defects: 1) Chitosan, sodium alginate, gelatin, carrageenan, etc. are widely used in natural polymer materials.

The biocompatibility of synthetic polymer materials is not high, such as acrylamide monomer is neurotoxic to organisms

3) The structure of the inorganic carrier material is not easy to control, which affects mass transfer, and the ability to bind enzymes is poor, which has a greater impact on enzyme activity

The disadvantage is that the magnetic material has poor biocompatibility and cannot provide a good hydrophilic environment for the protein, which is not conducive to the biological activity of the enzyme; and the magnetic material cannot resist protein adsorption. When used to immobilize the enzyme carrier 2) The hydrophilic carrier has good biocompatibility and can improve the affinity of the substrate for the enzyme, which is beneficial for the enzyme Promoting reactions create a favorable microenvironment

But so far no one has applied it to the field of enzyme immobilization

Moreover, simply using zwitterionic materials as enzyme immobilization carriers is not easy to separate from the reaction system and does not have reproducibility

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