Method for preparing protein magnetic blotting nanospheres

Abstract

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

Description

technical field [0001] The invention relates to the preparation of a protein molecularly imprinted polymer. In particular, it involves a protein magnetic imprinting nanometer that has certain specific adsorption properties for template proteins, uses magnetism as a carrier, can be rapidly separated under the action of an external magnetic field, can avoid traditional complicated operations such as centrifugation, and simplifies the separation and recognition process. Ball preparation method. Background technique [0002] Molecular Imprinting Technique (MIT) is a technology for preparing polymer compounds (Molecular Imprinted Polymers, MIPs) with specific predetermined selectivity for specific target molecules (template molecules, also known as imprinted molecules) (M.D. Yan, O. Ramstrom, Ed., Molecularly Imprinted Materials Science and Technology, Marcel Dekker, New York, 2005). Molecularly imprinted polymers have holes whose shape matches the substrate molecules, and func...

AI Technical Summary

Problems solved by technology

However, due to the large particle size and relatively low specific surface area of ​​silicon spheres, some recognition sites are easily embedded in them, resulting in low adsorption capacity, and the use of silicon spheres as the separation medium involves cumbersome operations such as centrifugation, which is not conducive to wide application.

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