Anionic framework polymer for extracting muramidase, preparation method of anionic framework polymer

Abstract

The invention discloses an anionic framework polymer for extracting muramidase, a preparation method of the anionic framework polymer, and an application of the anionic framework polymer in the muramidase extraction. A special three-dimensional framework structure of existing melamine sponge is used; a framework is wrapped with a chitosan coating; diethylene glycol diglycidyl ether reacts with the chitosan coating, so that the chitosan coating is crosslinked, and the surface of the chitosan coating is provided with suspended epoxy groups; the epoxy groups react with sodium hydrogen sulfite to obtain sulfonate anions; in an ion exchange step in the muramidase extraction, the purification operation is carried out by taking the anionic framework polymer as an integral column, so that the surface of a material and muramidase are subjected to electrostatic interaction, the defect of poor diffusivity of muramidase macromolecules is overcome, the adsorption capacity of the material is improved, and the production efficiency of the muramidase is improved. A framework material has the advantages that the operation is easy and convenient, the repeated use is realized, the cost is low and the efficiency is high.

Description

technical field [0001] The invention relates to the technical field of bioactive substance extraction, in particular to a method for rapidly and efficiently extracting lysozyme by using an anionic skeleton polymer and a preparation method for the polymer material. Background technique [0002] Lysozyme is a hydrolase that specifically acts on the cell wall of microorganisms. It can cut off the link between the β-1,4-glycosidic bonds between N-acetylglucosamine and N-acetylmuramic acid in peptidoglycan, destroying The peptidoglycan scaffold, which cracks the cell wall, causes bacterial lysis and is thus a very effective antimicrobial agent. Lysozyme can be used as a preservative and antistaling agent for cooked aquatic products and cooked meat products. Adding lysozyme to cakes, beverages and fruit juices with a pH of 6.0 to 7.5 can also prevent microbial reproduction. Lysozyme has the function of destroying the structure of the bacterial cell wall, and this enzyme is used t...

AI Technical Summary

Problems solved by technology

[0004] The primary technical problem to be solved by the present invention is to provide a solution for the problem that the pore size of the ion exchange resin is relatively small in the process of extracting lysozyme from egg white by the existing ion exchange method. Anionic skeleton polymer, which has a three-dimensional network structure and 100 micron through-holes, has a large amount of negative charges on its surface, which can be quickly infiltrated by viscous egg whites, maximizing the adsorption of lysozyme molecules and avoiding the problem of diffusion rate Affect the adsorption efficiency and rate, and can easily elute the adsorbed substances, realize the multiple recycling of materials, and improve the production efficiency of lysozyme

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