Composition with nutritional and moisturizing efficacies as well as preparation method and application of composition

A composition and efficacy technology, applied in the field of daily chemicals, can solve the problems of difficulty in controlling the molecular weight of degradation products, difficulty in obtaining active glucosamine, high price of specific enzymes, etc., achieve nutrition and moisturizing performance, and easy absorption , low-cost effect

Active Publication Date: 2015-12-02
广州增城潮徽生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical degradation method is simple and cheap, but it is difficult to control the relative molecular weight of the degradation products, and it is difficult to obtain the required active glucosamine; the chemical synthesis method has a relatively high yield of active oligoglucosamine, but there are intermediate by-products. residual
The preparation of chitosan oligosaccharides by enzymatic degradation has recently received great attention. However, due to the high price of specific enzymes and the difficulty of commercialization, it is necessary to find other non-specific enzymes with good degradation effects and low prices, and use physical and chemical methods Combining with the enzymatic method to explore a compound degradation method that can efficiently and safely produce oligoglucosamine has become a breakthrough to solve the problem
[0004] Oligoglucosamine is distributed in almost all tissues of the human body and participates in the construction of human tissues and cell membranes, especially the intermediates in the synthesis of those articular cartilage and synovial fluid molecules. In vitro studies have shown that oligoglucosamine has clinical anti-osteoarthritis effects (compound Glucosamine hydrochloride treatment of osteoarthritis multi-center randomized double-blind trial), anti-tumor effect (D-glucosamine hydrochloride anti-tumor and immune regulation in vitro), anti-oxidation, liver protection (glucosamine hydrochloride on CCl4 Induced liver damage in mice), oligoglucosamine has been used clinically for a long time, but its research in cosmetics is rare

Method used

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  • Composition with nutritional and moisturizing efficacies as well as preparation method and application of composition
  • Composition with nutritional and moisturizing efficacies as well as preparation method and application of composition
  • Composition with nutritional and moisturizing efficacies as well as preparation method and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) with 2 mass parts chitosan (deacetylation degree is 92.0%, molecular weight 8 * 10 4 ) was dissolved in 200 parts by mass of 0.2mol / L, pH3 acetate buffer, 80 Hz ultrasonic wave for 15min, then added 0.005 parts by mass of lysozyme (Sigma-Aldrich, the same below), 0.005 parts by mass of cellulase (Sigma-Aldrich, The same below), reacted at 40°C for 4h; then added 0.0025 parts by mass of chitosanase (Sigma-Aldrich, the same below) and 0.0025 parts by mass of pectinase (Novozymes, Denmark, the same below), and reacted at 60°C 6h. The stirring state of 150rpm was maintained throughout the enzymolysis reaction. After the enzymolysis reaction was completed, the enzymolysis solution was heated at 95°C for 5 minutes to inactivate, then the temperature was lowered to 45°C, and 2 parts by mass of activated carbon was added. The decolorization time was 1 hour, and the activated carbon was filtered off. Finally, it was centrifuged at 3000 rpm, and the supernatant was taken to ...

Embodiment 2

[0050] (1) with 2 mass parts chitosan (deacetylation degree is 91.5%, molecular weight 12 * 10 4 ) was dissolved in 400 parts by mass of 0.2 mol / L acetate buffer solution with a pH of 5.5, ultrasonicated at 120 Hz for 45 minutes, then 0.1 parts by mass of lysozyme and 0.1 parts by mass of cellulase were added, and reacted at 55°C for 8 hours; then added 0.05 Parts by mass of chitosanase and 0.05 part by mass of pectinase were reacted at 70° C. for 12 hours. Stirring at 250rpm was maintained throughout the enzymolysis reaction. After the enzymolysis reaction, the enzymolysis solution was heated at 100°C for 10 minutes to inactivate it, then cooled to 55°C, and 4 parts by mass of activated carbon was added. The decolorization time was 2 hours, and the activated carbon was filtered off. Finally, it was centrifuged at a rotational speed of 5000 rpm, and the supernatant liquid was taken to obtain 350 parts by mass of an aqueous solution of glucosamine oligosaccharides with a chroma...

Embodiment 3

[0056] (1) with 2 mass parts chitosan (deacetylation degree is 92.7%, molecular weight 20 * 10 4 ) was dissolved in 300 parts by mass of 0.2 mol / L acetate buffer solution with a pH of 4.5, 100 Hz ultrasonic wave was used for 30 minutes, then 0.05 parts by mass of lysozyme and 0.05 parts by mass of cellulase were added, and the reaction was carried out at 50°C for 6 hours; then 0.0025 Parts by mass of chitosanase and 0.0025 parts by mass of pectinase were reacted at 65° C. for 8 hours. Stirring at 200rpm was maintained throughout the enzymolysis reaction. After the enzymolysis reaction, the enzymolysis solution was heated at 98°C for 8 minutes to inactivate, then cooled to 50°C, and 3 parts by mass of activated carbon was added. The decolorization time was 1.5h, and the activated carbon was filtered off. Finally, it was centrifuged at a rotational speed of 4500 rpm, and the supernatant was taken to obtain 250 parts by mass of an aqueous solution of glucosamine oligosaccharides ...

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Abstract

The invention discloses a composition with nutritional and moisturizing efficacies as well as a preparation method and application of the composition. The preparation method comprises the following steps: conducting ultrasonic treatment on chitosan, utilizing muramidase and cellulase for first-stage enzymolysis, and then utilizing chitosanase and pectinase for second-stage enzymolysis; after the enzymolysis reactions are finished, conducting inactivation, decolorization, solid-liquid separation and liquid taking on an enzymatic hydrolysate to obtain a chitooligosaccharides aqueous solution; uniformly mixing a beetroot extract, the chitooligosaccharides aqueous solution, methylisothiazolinone and phenoxyethanol to obtain the composition. The composition has a remarkable effect of nourishing skin and the remarkable capability of promoting the growth of fibroblasts affecting skin aging in the dermis layer; the composition can be utilized for preparation of daily cosmetics, and the market potential of the prepared daily cosmetics is great.

Description

technical field [0001] The invention belongs to the technical field of daily chemicals, and in particular relates to a composition with nutritional and moisturizing effects, a preparation method and application thereof. Background technique [0002] Known as the "sixth vital element of the human body" and "soft gold", chitin (Chitin) widely exists in seafood and filamentous fungi. However, due to its insolubility in water, its development and application are greatly restricted. Glucosamine oligosaccharides (also known as chitooligosaccharides and chitooligosaccharides) are obtained from the degradation of chitosan, a product of chitin deacetylation, and are formed by linking 2 to 20 glucosamines through β-1-4 glycosidic bonds. Oligosaccharides are the only basic aminopolysaccharides that exist in large quantities in natural sugars. They have good water solubility and are easily absorbed by objects. [0003] The main methods of degrading chitosan to prepare low-molecular-we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K8/73A61Q19/00
CPCA61K8/736A61K8/97A61K2800/10A61Q19/00
Inventor 郑华生姚丽凤谢明容陈杰烽谢培镇张鹏
Owner 广州增城潮徽生物技术有限公司
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